Organelle DNA haplotypes reflect crop-use characteristic and geographic origins of Cannabis sativa

Simon Gilmore, Rod Peakall, James ROBERTSON

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Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis
Original languageEnglish
Pages (from-to)179-190
Number of pages12
JournalForensic Science International
Issue number2-3
Publication statusPublished - 2007
Externally publishedYes


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