Organelle DNA haplotypes reflect crop-use characteristic and geographic origins of Cannabis sativa

Simon Gilmore; Rod Peakall; James ROBERTSON

doi:10.1016/j.forsciint.2006.10.025

Organelle DNA haplotypes reflect crop-use characteristic and geographic origins of Cannabis sativa

Simon Gilmore, Rod Peakall, James ROBERTSON

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis

Original language	English
Pages (from-to)	179-190
Number of pages	12
Journal	Forensic Science International
Volume	172
Issue number	2-3
DOIs	https://doi.org/10.1016/j.forsciint.2006.10.025
Publication status	Published - 2007
Externally published	Yes

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10.1016/j.forsciint.2006.10.025

Cite this

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title = "Organelle DNA haplotypes reflect crop-use characteristic and geographic origins of Cannabis sativa",

abstract = "Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis",

author = "Simon Gilmore and Rod Peakall and James ROBERTSON",

year = "2007",

doi = "10.1016/j.forsciint.2006.10.025",

language = "English",

volume = "172",

pages = "179--190",

journal = "Forensic Science International",

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publisher = "Elsevier Ireland Ltd",

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T1 - Organelle DNA haplotypes reflect crop-use characteristic and geographic origins of Cannabis sativa

AU - Gilmore, Simon

AU - Peakall, Rod

AU - ROBERTSON, James

PY - 2007

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N2 - Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis

AB - Comparative sequencing of cannabis individuals across 12 chloroplast and mitochondrial DNA loci revealed 7 polymorphic sites, including 5 length variable regions and 2 single nucleotide polymorphisms. Simple PCR assays were developed to assay these polymorphisms, and organelle DNA haplotypes were obtained for 188 cannabis individuals from 76 separate populations, including drug-type, fibre-type and wild populations. The haplotype data were analysed using parsimony, UPGMA and neighbour joining methods. Three haplotype groups were recovered by each analysis method, and these groups are suggestive of the crop-use characteristics and geographical origin of the populations, although not strictly diagnostic. We discuss the relationship between our haplotype data and taxonomic opinions of cannabis, and the implications of organelle DNA haplotyping to forensic investigations of cannabis

U2 - 10.1016/j.forsciint.2006.10.025

DO - 10.1016/j.forsciint.2006.10.025

M3 - Article

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JO - Forensic Science International

JF - Forensic Science International

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