Overlapping gene expression profiles in rheumatoid fibroblast-like synviocytes induces by the proinflammatory cytokines interleukin-1 beta and tumor necrosis factor

Megan Taberner, Kieran F. Scott, Lilach Weininger, Charles R. Mackay, Michael ROLPH

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Objective and Design: The development of therapies directed against TNFα and IL-1β has underscored the importance of these cytokines in rheumatoid arthritis (RA). In this study, oligonucleotide microarrays were used to identify novel transcriptional events mediated by TNFα and IL-1β. Methods: In this study we have used Affymetrix U95A GeneChips representing 12,600 full-length human genes to identify transcriptional events mediated by these cytokines. Fibroblast-like synoviocytes were cultured from rheumatoid synovium from RA patients and stimulated with TNFα and IL-1β. Gene transcript levels were determined using Affymetrix U95A GeneChips representing 12,600 full-length human genes. Results: A large number of differentially regulated genes were identified (1.7% of array-displayed genes for TNFα and 2.4% for IL-1β), and the validity of the array protocol was subsequently confirmed using real-time PCR. The majority of the differentially expressed genes were regulated by both TNFα and IL-1β, reflecting the distal signaling pathways shared by these cytokines. A large number of novel TNFα and IL-1β-regulated genes were identified. Conclusions: A panel of novel TNFα- and IL-1β-regulated genes was identified, and these are promising candidates for further study in relation to RA and other inflammatory diseases
Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalIAHS Proceedings and Reports
Volume54
DOIs
Publication statusPublished - 2005
Externally publishedYes

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