TY - JOUR
T1 - PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus
AU - Wang, Wei
AU - Song, Zhigang
AU - Guan, Wencai
AU - Liu, Yi
AU - Zhang, Xiaonan
AU - Xu, Lei
AU - Li, Jianhua
AU - Yuan, Zhenghong
AU - Hu, Yunwen
PY - 2014/5
Y1 - 2014/5
N2 - Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
AB - Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
KW - Antiviral Agents/pharmacology
KW - Drug Resistance, Viral/genetics
KW - Humans
KW - Influenza A Virus, H7N9 Subtype/drug effects
KW - Influenza, Human/drug therapy
KW - Mutation
KW - Neuraminidase/genetics
KW - Oseltamivir/pharmacology
KW - Polymerase Chain Reaction
KW - Viral Proteins/genetics
U2 - 10.3201/eid2005.131364
DO - 10.3201/eid2005.131364
M3 - Short Survey/Scientific Report
C2 - 24751348
VL - 20
SP - 847
EP - 849
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
SN - 1080-6040
IS - 5
ER -