PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus

Wei Wang; Zhigang Song; Wencai Guan; Yi Liu; Xiaonan Zhang; Lei Xu; Jianhua Li; Zhenghong Yuan; Yunwen Hu

doi:10.3201/eid2005.131364

PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus

Wei Wang
, Zhigang Song
, Wencai Guan
, Yi Liu
, Xiaonan Zhang
, Lei Xu
, Jianhua Li
, Zhenghong Yuan
, Yunwen Hu

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

Original language	English
Pages (from-to)	847-849
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	20
Issue number	5
DOIs	https://doi.org/10.3201/eid2005.131364
Publication status	Published - May 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.3201/eid2005.131364

Cite this

@article{0e5e331cfc1848f1a838b3375aa5d295,

title = "PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus",

abstract = "Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens. ",

keywords = "Antiviral Agents/pharmacology, Drug Resistance, Viral/genetics, Humans, Influenza A Virus, H7N9 Subtype/drug effects, Influenza, Human/drug therapy, Mutation, Neuraminidase/genetics, Oseltamivir/pharmacology, Polymerase Chain Reaction, Viral Proteins/genetics",

author = "Wei Wang and Zhigang Song and Wencai Guan and Yi Liu and Xiaonan Zhang and Lei Xu and Jianhua Li and Zhenghong Yuan and Yunwen Hu",

year = "2014",

month = may,

doi = "10.3201/eid2005.131364",

language = "English",

volume = "20",

pages = "847--849",

journal = "Emerging Infectious Diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention (CDC)",

number = "5",

}

TY - JOUR

T1 - PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus

AU - Wang, Wei

AU - Song, Zhigang

AU - Guan, Wencai

AU - Liu, Yi

AU - Zhang, Xiaonan

AU - Xu, Lei

AU - Li, Jianhua

AU - Yuan, Zhenghong

AU - Hu, Yunwen

PY - 2014/5

Y1 - 2014/5

N2 - Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

AB - Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

KW - Antiviral Agents/pharmacology

KW - Drug Resistance, Viral/genetics

KW - Humans

KW - Influenza A Virus, H7N9 Subtype/drug effects

KW - Influenza, Human/drug therapy

KW - Mutation

KW - Neuraminidase/genetics

KW - Oseltamivir/pharmacology

KW - Polymerase Chain Reaction

KW - Viral Proteins/genetics

UR - https://www.scopus.com/pages/publications/84898910612

U2 - 10.3201/eid2005.131364

DO - 10.3201/eid2005.131364

M3 - Article

C2 - 24751348

SN - 1080-6040

VL - 20

SP - 847

EP - 849

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 5

ER -

PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this