Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

Hilary S. Warren, Fan WU, Peggy L. Horn, David Pyne, Nicholas P. West, Allan W. Cripps

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NM cell function. Loss of NM cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NM cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%)
Original languageEnglish
Pages (from-to)68-70
Number of pages3
JournalJournal of Immunological Methods
Volume392
Issue number1-2
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Natural Killer Cells
Blood Cells
Antibodies
Flow Cytometry
Tissue Donors
Lymphocytes
Population

Cite this

Warren, Hilary S. ; WU, Fan ; Horn, Peggy L. ; Pyne, David ; West, Nicholas P. ; Cripps, Allan W. / Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay. In: Journal of Immunological Methods. 2013 ; Vol. 392, No. 1-2. pp. 68-70.
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abstract = "In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NM cell function. Loss of NM cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11{\%} for natural killing and 3{\%} for antibody-dependent killing, compared to 14{\%} and 9{\%} respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NM cells in the population both for natural killing (CV 33{\%}) and antibody-dependent killing (CV 27{\%})",
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Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay. / Warren, Hilary S.; WU, Fan; Horn, Peggy L.; Pyne, David; West, Nicholas P.; Cripps, Allan W.

In: Journal of Immunological Methods, Vol. 392, No. 1-2, 2013, p. 68-70.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay

AU - Warren, Hilary S.

AU - WU, Fan

AU - Horn, Peggy L.

AU - Pyne, David

AU - West, Nicholas P.

AU - Cripps, Allan W.

PY - 2013

Y1 - 2013

N2 - In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NM cell function. Loss of NM cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NM cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%)

AB - In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NM cell function. Loss of NM cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NM cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%)

KW - ADCC

KW - Cytotoxicity

KW - Human NK cells

KW - Rituximab

KW - TINKL

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SP - 68

EP - 70

JO - Immunotechnology

JF - Immunotechnology

SN - 0022-1759

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ER -