Physical map of two tammar wallaby chromosomes: A strategy for mapping in non-model mammals

Janine Deakin, Edda Koina, Paul Waters, Ruth Doherty, V Patel, Margaret Delbridge, Bianca Dobson, James Fong, Yanqiu Hu, Cecilia van den Hurk, Andrew Pask, Geoff Shaw, Carly Smith, Katherine Thompson, Matthew Wakefield, Hongshi Yu, Marilyn Renfree, Jennifer Marshall Graves

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Marsupials are especially valuable for comparative genomic studies of mammals. Two distantly related model marsupials have been sequenced: the South American opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii), which last shared a common ancestor about 70 Mya. The six-fold opossum genome sequence has been assembled and assigned to chromosomes with the help of a cytogenetic map. A good cytogenetic map will be even more essential for assembly and anchoring of the two-fold wallaby genome. As a start to generating a physical map of gene locations on wallaby chromosomes, we focused on two hromosomes sharing homology with the human X, wallaby chromosomes X and 5. We devised an efficient strategy for mapping large conserved synteny blocks in non-model mammals, and applied this to generate dense maps of the X and Fneo-X_ regions and to determine the arrangement of large conserved synteny blocks on chromosome 5. Comparisons between the wallaby and opossum chromosome maps revealed many rearrangements, highlighting the need for comparative gene mapping between South American and Australian marsupials. Frequent rearrangement of the X, along with the absence of a marsupial XIST gene, suggests that inactivation of the marsupial X chromosome does not depend on a whole-chromosome repression by a control locus.
Original languageEnglish
Pages (from-to)1159-1175
Number of pages17
JournalChromosome Research
Volume16
DOIs
Publication statusPublished - 2008
Externally publishedYes

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    Deakin, J., Koina, E., Waters, P., Doherty, R., Patel, V., Delbridge, M., Dobson, B., Fong, J., Hu, Y., van den Hurk, C., Pask, A., Shaw, G., Smith, C., Thompson, K., Wakefield, M., Yu, H., Renfree, M., & Marshall Graves, J. (2008). Physical map of two tammar wallaby chromosomes: A strategy for mapping in non-model mammals. Chromosome Research, 16, 1159-1175. https://doi.org/10.1007/s10577-008-1266-y