Physical Mapping of Immune Genes in the Tammar Wallaby (Macropus eugenii)

Claire Sanderson, Katherine Belov, Janine Deakin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative enomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.
Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalCytogenetic and Genome Research
Volume127
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Macropodidae
Chromosome Mapping
Bacterial Artificial Chromosomes
Marsupialia
Genes
Genome
Chromosomes
Chromosome Painting
T-Cell Receptor Genes
Immunoglobulin Genes
Fluorescence In Situ Hybridization
Light

Cite this

@article{c42e84e70e074354a033730652cc7c55,
title = "Physical Mapping of Immune Genes in the Tammar Wallaby (Macropus eugenii)",
abstract = "The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative enomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.",
author = "Claire Sanderson and Katherine Belov and Janine Deakin",
year = "2009",
doi = "10.1159/000260372",
language = "English",
volume = "127",
pages = "21--25",
journal = "Cytogenetics and Cell Genetics",
issn = "0301-0171",
publisher = "S Karger AG",

}

Physical Mapping of Immune Genes in the Tammar Wallaby (Macropus eugenii). / Sanderson, Claire; Belov, Katherine; Deakin, Janine.

In: Cytogenetic and Genome Research, Vol. 127, 2009, p. 21-25.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Physical Mapping of Immune Genes in the Tammar Wallaby (Macropus eugenii)

AU - Sanderson, Claire

AU - Belov, Katherine

AU - Deakin, Janine

PY - 2009

Y1 - 2009

N2 - The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative enomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.

AB - The tammar wallaby (Macropus eugenii) is a model marsupial that has recently had its genome sequenced to a depth of 2-fold coverage. Although this is a great resource for comparative enomic studies, information on gene location is essential if this resource is to be used to its full potential. In this study, tammar wallaby bacterial artificial chromosomes (BACs) containing key immune genes were isolated from the tammar wallaby BAC library. BACs containing T cell receptor (TCR) and immunoglobulin (Ig) genes were physically mapped using fluorescence in situ hybridisation (FISH) to tammar wallaby chromosomes. Congruence between the locations of these immune genes in the tammar wallaby genome, with those predicted from chromosome painting data, highlights the conservation of genomic context of these important immune genes in marsupials. The isolation and mapping of these key immune genes in the tammar wallaby will aid in the assembly of the recently sequenced light coverage genome and assignment of sequence to chromosomes.

U2 - 10.1159/000260372

DO - 10.1159/000260372

M3 - Article

VL - 127

SP - 21

EP - 25

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

SN - 0301-0171

ER -