TY - JOUR
T1 - Physicochemical, antioxidant and anti-cancer activity of a Eucalyptus robusta (Sm.) leaf aqueous extract
AU - Vuong, Quan V.
AU - Hirun, Sathira
AU - Chuen, Tiffany L.K.
AU - Goldsmith, Chloe D.
AU - Munro, Benjamin
AU - Bowyer, Michael C.
AU - Chalmers, Anita C.
AU - Sakoff, Jennette A.
AU - Phillips, Phoebe A.
AU - Scarlett, Christopher J.
N1 - Funding Information:
We acknowledge the following funding support: Ramaciotti Foundation ( ES2012/0104 ); Cancer Australia and Cure Cancer Australia Foundation ( 1033781 ). PAP is supported by a National Health and Medical Research Council Career Development Fellowship.
Publisher Copyright:
© 2014 Elsevier B.V..
PY - 2015
Y1 - 2015
N2 - Eucalyptus robusta (Sm.) (ER) is a widely distributed tree native to the east coast of Australia, which has also been established in numerous other countries. ER leaves contain high levels of essential oils and are rich in total phenolic compounds (TPC), which have been linked with health benefits; however, there is limited information on the bioactivity of ER leaf extracts. This study aimed to optimise water extraction conditions for TPC, prepare a spray-dried powdered extract and test its physicochemical, antioxidant and anti-proliferative properties. The results showed that optimal water extraction conditions for TPC were 85. °C, 15. min and a water-to-leaf ratio of 20:1. mL/g. Under these conditions, spray-dried powdered extract was prepared with a recovery yield of 85%. The extract was water-soluble and had a TPC level of 407. mg. GAE/g. It also possessed potent antioxidant capacity, comparable to pure ascorbic acid, but higher than pure α-tocopherol. In addition, the powdered extract demonstrated significant activity against a panel of cancer cell lines, which included cancers of the pancreas, breast, lung, brain, skin, colon and ovary. Of note, the ER extract exerted a more significant toxic effect on pancreatic cancer (PC) cells compared to gemcitabine, the first line chemotherapeutic agent for PC. We suggest that future studies should purify individual bioactive compounds from ER for further investigation of its potential health promoting and anti-cancer activity.
AB - Eucalyptus robusta (Sm.) (ER) is a widely distributed tree native to the east coast of Australia, which has also been established in numerous other countries. ER leaves contain high levels of essential oils and are rich in total phenolic compounds (TPC), which have been linked with health benefits; however, there is limited information on the bioactivity of ER leaf extracts. This study aimed to optimise water extraction conditions for TPC, prepare a spray-dried powdered extract and test its physicochemical, antioxidant and anti-proliferative properties. The results showed that optimal water extraction conditions for TPC were 85. °C, 15. min and a water-to-leaf ratio of 20:1. mL/g. Under these conditions, spray-dried powdered extract was prepared with a recovery yield of 85%. The extract was water-soluble and had a TPC level of 407. mg. GAE/g. It also possessed potent antioxidant capacity, comparable to pure ascorbic acid, but higher than pure α-tocopherol. In addition, the powdered extract demonstrated significant activity against a panel of cancer cell lines, which included cancers of the pancreas, breast, lung, brain, skin, colon and ovary. Of note, the ER extract exerted a more significant toxic effect on pancreatic cancer (PC) cells compared to gemcitabine, the first line chemotherapeutic agent for PC. We suggest that future studies should purify individual bioactive compounds from ER for further investigation of its potential health promoting and anti-cancer activity.
KW - Antioxidant
KW - Eucalyptus robusta (Sm.)
KW - Pancreatic cancer
KW - Polyphenols
UR - http://www.scopus.com/inward/record.url?scp=84937562156&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2014.10.061
DO - 10.1016/j.indcrop.2014.10.061
M3 - Article
AN - SCOPUS:84937562156
SN - 0926-6690
VL - 64
SP - 167
EP - 174
JO - Industrial Crops and Products
JF - Industrial Crops and Products
IS - 1
ER -