Population structure of island and mainland populations of the quokka, Setonix brachyurus (Macropodidae): a comparison of AFLP and microsatellite markers

Erika Alacs, Peter Spencer, Paul de Tores, Siegfried Krauss

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Translocation and reintroduction are important tools for the conservation or recovery of species threatened with extinction in the wild. However, an understanding of the potential genetic consequences of mixing populations requires an understanding of the genetic variation within, and similarities among, donor and recipient populations. Genetic diversity was measured using two independent marker systems (microsatellites and AFLPs) for one island and four small remnant mainland populations of Setonix brachyurus, a threatened medium sized macropod restricted to fragmented habitat remnants and two off-shore islands in southwest Australia. Microsatellite diversity in the island population (R s = 3.2, H e = 71%) was similar to, or greater than, all mainland populations (R s = 2.1â¿¿3.9, H e = 34-71%). In contrast, AFLP diversity was significantly lower in the island population (PPL = 20.5; H j = 0.118) compared to all mainland populations (mean PPL = 79.5â¿¿89.7; mean H j = 0.23â¿¿0.29). Microsatellites differentiated all (mainland and island) populations from each other. However, AFLP only differentiated the island population from the mainland populationsâ¿¿all mainland populations were not significantly differentiated from each other for this marker. Given a known time since isolation of the island population from the mainland (6,000 years ago), and an overall more conservative rate of evolution of AFLP markers, our results are consistent with mainland populations fragmenting thousands of years ago (but
    Original languageEnglish
    Pages (from-to)297-309
    Number of pages13
    JournalConservation Genetics
    Volume12
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Macropodidae
    Islands
    Microsatellite Repeats
    amplified fragment length polymorphism
    population structure
    microsatellite repeats
    Population
    reintroduction
    marker
    comparison
    translocation
    genetic variation
    extinction
    Endangered Species
    threatened species
    habitat fragmentation
    habitat
    Ecosystem

    Cite this

    @article{c054cb4d78e0434cbdb1f7c7908c83e3,
    title = "Population structure of island and mainland populations of the quokka, Setonix brachyurus (Macropodidae): a comparison of AFLP and microsatellite markers",
    abstract = "Translocation and reintroduction are important tools for the conservation or recovery of species threatened with extinction in the wild. However, an understanding of the potential genetic consequences of mixing populations requires an understanding of the genetic variation within, and similarities among, donor and recipient populations. Genetic diversity was measured using two independent marker systems (microsatellites and AFLPs) for one island and four small remnant mainland populations of Setonix brachyurus, a threatened medium sized macropod restricted to fragmented habitat remnants and two off-shore islands in southwest Australia. Microsatellite diversity in the island population (R s = 3.2, H e = 71{\%}) was similar to, or greater than, all mainland populations (R s = 2.1{\^a}¿¿3.9, H e = 34-71{\%}). In contrast, AFLP diversity was significantly lower in the island population (PPL = 20.5; H j = 0.118) compared to all mainland populations (mean PPL = 79.5{\^a}¿¿89.7; mean H j = 0.23{\^a}¿¿0.29). Microsatellites differentiated all (mainland and island) populations from each other. However, AFLP only differentiated the island population from the mainland populations{\^a}¿¿all mainland populations were not significantly differentiated from each other for this marker. Given a known time since isolation of the island population from the mainland (6,000 years ago), and an overall more conservative rate of evolution of AFLP markers, our results are consistent with mainland populations fragmenting thousands of years ago (but",
    keywords = "Marsupial, Genetic variation, Historical fragmentation, Conservation, Management",
    author = "Erika Alacs and Peter Spencer and {de Tores}, Paul and Siegfried Krauss",
    year = "2011",
    doi = "10.1007/s10592-010-0140-6",
    language = "English",
    volume = "12",
    pages = "297--309",
    journal = "Conservation Genetics",
    issn = "1566-0621",
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    }

    Population structure of island and mainland populations of the quokka, Setonix brachyurus (Macropodidae): a comparison of AFLP and microsatellite markers. / Alacs, Erika; Spencer, Peter; de Tores, Paul; Krauss, Siegfried.

    In: Conservation Genetics, Vol. 12, 2011, p. 297-309.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Population structure of island and mainland populations of the quokka, Setonix brachyurus (Macropodidae): a comparison of AFLP and microsatellite markers

    AU - Alacs, Erika

    AU - Spencer, Peter

    AU - de Tores, Paul

    AU - Krauss, Siegfried

    PY - 2011

    Y1 - 2011

    N2 - Translocation and reintroduction are important tools for the conservation or recovery of species threatened with extinction in the wild. However, an understanding of the potential genetic consequences of mixing populations requires an understanding of the genetic variation within, and similarities among, donor and recipient populations. Genetic diversity was measured using two independent marker systems (microsatellites and AFLPs) for one island and four small remnant mainland populations of Setonix brachyurus, a threatened medium sized macropod restricted to fragmented habitat remnants and two off-shore islands in southwest Australia. Microsatellite diversity in the island population (R s = 3.2, H e = 71%) was similar to, or greater than, all mainland populations (R s = 2.1â¿¿3.9, H e = 34-71%). In contrast, AFLP diversity was significantly lower in the island population (PPL = 20.5; H j = 0.118) compared to all mainland populations (mean PPL = 79.5â¿¿89.7; mean H j = 0.23â¿¿0.29). Microsatellites differentiated all (mainland and island) populations from each other. However, AFLP only differentiated the island population from the mainland populationsâ¿¿all mainland populations were not significantly differentiated from each other for this marker. Given a known time since isolation of the island population from the mainland (6,000 years ago), and an overall more conservative rate of evolution of AFLP markers, our results are consistent with mainland populations fragmenting thousands of years ago (but

    AB - Translocation and reintroduction are important tools for the conservation or recovery of species threatened with extinction in the wild. However, an understanding of the potential genetic consequences of mixing populations requires an understanding of the genetic variation within, and similarities among, donor and recipient populations. Genetic diversity was measured using two independent marker systems (microsatellites and AFLPs) for one island and four small remnant mainland populations of Setonix brachyurus, a threatened medium sized macropod restricted to fragmented habitat remnants and two off-shore islands in southwest Australia. Microsatellite diversity in the island population (R s = 3.2, H e = 71%) was similar to, or greater than, all mainland populations (R s = 2.1â¿¿3.9, H e = 34-71%). In contrast, AFLP diversity was significantly lower in the island population (PPL = 20.5; H j = 0.118) compared to all mainland populations (mean PPL = 79.5â¿¿89.7; mean H j = 0.23â¿¿0.29). Microsatellites differentiated all (mainland and island) populations from each other. However, AFLP only differentiated the island population from the mainland populationsâ¿¿all mainland populations were not significantly differentiated from each other for this marker. Given a known time since isolation of the island population from the mainland (6,000 years ago), and an overall more conservative rate of evolution of AFLP markers, our results are consistent with mainland populations fragmenting thousands of years ago (but

    KW - Marsupial

    KW - Genetic variation

    KW - Historical fragmentation

    KW - Conservation

    KW - Management

    U2 - 10.1007/s10592-010-0140-6

    DO - 10.1007/s10592-010-0140-6

    M3 - Article

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    JO - Conservation Genetics

    JF - Conservation Genetics

    SN - 1566-0621

    ER -