Pre-clinical study protocol

Blood transfusion in endotoxaemic shock

Nchafatso G. Obonyo, Liam Byrne, John Paul Tung, Gabriela Simonova, Sara D. Diab, Kimble R. Dunster, Margaret R. Passmore, Ai Ching Boon, Louise See Hoe, Sanne Engkilde-Pedersen, Arlanna Esguerra-Lallen, Mohd H. Fauzi, Leticia P. Pimenta, Jonathan E. Millar, Jonathon P. Fanning, Frank Van Haren, Chris M. Anstey, Louise Cullen, Jacky Suen, Kiran Shekar & 2 others Kathryn Maitland, John F. Fraser

Research output: Contribution to journalArticle

Abstract

The Surviving Sepsis Campaign (SCC) and the American College of Critical Care Medicine (ACCM) guidelines recommend blood transfusion in sepsis when the haemoglobin concentration drops below 7.0 g/dL and 10.0 g/dL respectively, while the World Health Organisation (WHO) guideline recommends transfusion in septic shock ‘if intravenous (IV) fluids do not maintain adequate circulation’, as a supportive measure of last resort. Volume expansion using crystalloid and colloid fluid boluses for haemodynamic resuscitation in severe illness/sepsis, has been associated with adverse outcomes in recent literature. However, the volume expansion effect(s) following blood transfusion for haemodynamic circulatory support, in severe illness remain unclear with most previous studies having focused on evaluating effects of either different RBC storage durations (short versus long duration) or haemoglobin thresholds (low versus high threshold) pre-transfusion. • We describe the protocol for a pre-clinical randomised controlled trial designed to examine haemodynamic effect(s) of early volume expansion using packed RBCs (PRBCs) transfusion (before any crystalloids or colloids) in a validated ovine-model of hyperdynamic endotoxaemic shock. • Additional exploration of mechanisms underlying any physiological, haemodynamic, haematological, immunologic and tissue specific-effects of blood transfusion will be undertaken including comparison of effects of short (≤5 days) versus long (≥30 days) storage duration of PRBCs prior to transfusion.

Original languageEnglish
Pages (from-to)1124-1132
Number of pages9
JournalMethodsX
Volume6
DOIs
Publication statusPublished - 2019

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Hemodynamics
Clinical Protocols
Blood Transfusion
Shock
Blood
Sepsis
Colloids
Hemoglobins
Guidelines
Resuscitation
Fluids
Critical Care
Septic Shock
Medicine
Sheep
Randomized Controlled Trials
Health
Tissue
Clinical Studies
crystalloid solutions

Cite this

Obonyo, N. G., Byrne, L., Tung, J. P., Simonova, G., Diab, S. D., Dunster, K. R., ... Fraser, J. F. (2019). Pre-clinical study protocol: Blood transfusion in endotoxaemic shock. MethodsX, 6, 1124-1132. https://doi.org/10.1016/j.mex.2019.05.005
Obonyo, Nchafatso G. ; Byrne, Liam ; Tung, John Paul ; Simonova, Gabriela ; Diab, Sara D. ; Dunster, Kimble R. ; Passmore, Margaret R. ; Boon, Ai Ching ; See Hoe, Louise ; Engkilde-Pedersen, Sanne ; Esguerra-Lallen, Arlanna ; Fauzi, Mohd H. ; Pimenta, Leticia P. ; Millar, Jonathan E. ; Fanning, Jonathon P. ; Van Haren, Frank ; Anstey, Chris M. ; Cullen, Louise ; Suen, Jacky ; Shekar, Kiran ; Maitland, Kathryn ; Fraser, John F. / Pre-clinical study protocol : Blood transfusion in endotoxaemic shock. In: MethodsX. 2019 ; Vol. 6. pp. 1124-1132.
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Obonyo, NG, Byrne, L, Tung, JP, Simonova, G, Diab, SD, Dunster, KR, Passmore, MR, Boon, AC, See Hoe, L, Engkilde-Pedersen, S, Esguerra-Lallen, A, Fauzi, MH, Pimenta, LP, Millar, JE, Fanning, JP, Van Haren, F, Anstey, CM, Cullen, L, Suen, J, Shekar, K, Maitland, K & Fraser, JF 2019, 'Pre-clinical study protocol: Blood transfusion in endotoxaemic shock', MethodsX, vol. 6, pp. 1124-1132. https://doi.org/10.1016/j.mex.2019.05.005

Pre-clinical study protocol : Blood transfusion in endotoxaemic shock. / Obonyo, Nchafatso G.; Byrne, Liam; Tung, John Paul; Simonova, Gabriela; Diab, Sara D.; Dunster, Kimble R.; Passmore, Margaret R.; Boon, Ai Ching; See Hoe, Louise; Engkilde-Pedersen, Sanne; Esguerra-Lallen, Arlanna; Fauzi, Mohd H.; Pimenta, Leticia P.; Millar, Jonathan E.; Fanning, Jonathon P.; Van Haren, Frank; Anstey, Chris M.; Cullen, Louise; Suen, Jacky; Shekar, Kiran; Maitland, Kathryn; Fraser, John F.

In: MethodsX, Vol. 6, 2019, p. 1124-1132.

Research output: Contribution to journalArticle

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T2 - Blood transfusion in endotoxaemic shock

AU - Obonyo, Nchafatso G.

AU - Byrne, Liam

AU - Tung, John Paul

AU - Simonova, Gabriela

AU - Diab, Sara D.

AU - Dunster, Kimble R.

AU - Passmore, Margaret R.

AU - Boon, Ai Ching

AU - See Hoe, Louise

AU - Engkilde-Pedersen, Sanne

AU - Esguerra-Lallen, Arlanna

AU - Fauzi, Mohd H.

AU - Pimenta, Leticia P.

AU - Millar, Jonathan E.

AU - Fanning, Jonathon P.

AU - Van Haren, Frank

AU - Anstey, Chris M.

AU - Cullen, Louise

AU - Suen, Jacky

AU - Shekar, Kiran

AU - Maitland, Kathryn

AU - Fraser, John F.

PY - 2019

Y1 - 2019

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KW - Sepsis

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Obonyo NG, Byrne L, Tung JP, Simonova G, Diab SD, Dunster KR et al. Pre-clinical study protocol: Blood transfusion in endotoxaemic shock. MethodsX. 2019;6:1124-1132. https://doi.org/10.1016/j.mex.2019.05.005