Preservation of and DNA extraction from muscle tissue

Dennis MCNEVIN

Research output: A Conference proceeding or a Chapter in BookChapter

3 Citations (Scopus)

Abstract

As well as protecting DNA for subsequent analysis, tissue preservation methods ideally should be safe, readily available, and easy to transport at relatively low cost. Formalin (formaldehyde solution), used extensively to preserve medical and museum specimens, irreparably damages DNA. We have found four tissue preservatives (solid salt, salt-saturated dimethyl sulfoxide (DMSO)-EDTA solution, ethanol solution, and ethanol-EDTA solution) that preserved muscle tissue at 35 °C for up to 1 month: full short tandem repeat (STR) profiles were obtained after preservation. In addition, salt-saturated DMSO-EDTA solution yielded full STR profiles from aliquots of the liquid preservative surrounding muscle tissue.

Original languageEnglish
Title of host publicationForensic DNA Typing Protocols
EditorsWilliam Goodwin
Place of PublicationNew York
PublisherHumana Press
Pages43-53
Number of pages11
Volume1420
ISBN (Print)9781493935956
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

Fingerprint

Muscles
Edetic Acid
DNA
Salts
Dimethyl Sulfoxide
Microsatellite Repeats
Ethanol
Tissue Preservation
Museums
Formaldehyde
DNA Damage
Costs and Cost Analysis

Cite this

MCNEVIN, D. (2016). Preservation of and DNA extraction from muscle tissue. In W. Goodwin (Ed.), Forensic DNA Typing Protocols (Vol. 1420, pp. 43-53). (Methods in Molecular Biology). New York: Humana Press. https://doi.org/10.1007/978-1-4939-3597-0_4
MCNEVIN, Dennis. / Preservation of and DNA extraction from muscle tissue. Forensic DNA Typing Protocols. editor / William Goodwin. Vol. 1420 New York : Humana Press, 2016. pp. 43-53 (Methods in Molecular Biology).
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MCNEVIN, D 2016, Preservation of and DNA extraction from muscle tissue. in W Goodwin (ed.), Forensic DNA Typing Protocols. vol. 1420, Methods in Molecular Biology, Humana Press, New York, pp. 43-53. https://doi.org/10.1007/978-1-4939-3597-0_4

Preservation of and DNA extraction from muscle tissue. / MCNEVIN, Dennis.

Forensic DNA Typing Protocols. ed. / William Goodwin. Vol. 1420 New York : Humana Press, 2016. p. 43-53 (Methods in Molecular Biology).

Research output: A Conference proceeding or a Chapter in BookChapter

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MCNEVIN D. Preservation of and DNA extraction from muscle tissue. In Goodwin W, editor, Forensic DNA Typing Protocols. Vol. 1420. New York: Humana Press. 2016. p. 43-53. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3597-0_4