Preservation of and DNA extraction from muscle tissue

Dennis MCNEVIN

    Research output: A Conference proceeding or a Chapter in BookChapterpeer-review

    7 Citations (Scopus)

    Abstract

    As well as protecting DNA for subsequent analysis, tissue preservation methods ideally should be safe, readily available, and easy to transport at relatively low cost. Formalin (formaldehyde solution), used extensively to preserve medical and museum specimens, irreparably damages DNA. We have found four tissue preservatives (solid salt, salt-saturated dimethyl sulfoxide (DMSO)-EDTA solution, ethanol solution, and ethanol-EDTA solution) that preserved muscle tissue at 35 °C for up to 1 month: full short tandem repeat (STR) profiles were obtained after preservation. In addition, salt-saturated DMSO-EDTA solution yielded full STR profiles from aliquots of the liquid preservative surrounding muscle tissue.

    Original languageEnglish
    Title of host publicationForensic DNA Typing Protocols
    EditorsWilliam Goodwin
    Place of PublicationNew York
    PublisherHumana Press
    Pages43-53
    Number of pages11
    Volume1420
    ISBN (Print)9781493935956
    DOIs
    Publication statusPublished - 2016

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    ISSN (Print)1064-3745

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