Primers for detection of Macquarie perch from environmental and trace DNA samples

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.
    Original languageEnglish
    Pages (from-to)551-553
    Number of pages3
    JournalConservation Genetics Resources
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Macquaria australasica
    Perches
    Fishes
    DNA
    predation
    fish
    Salmonidae
    sampling
    DNA Primers
    Fresh Water
    Rivers
    freshwater fish
    Polymerase Chain Reaction
    rivers
    detection
    river

    Cite this

    @article{23accb3390eb45c6b99a67bddd5ab643,
    title = "Primers for detection of Macquarie perch from environmental and trace DNA samples",
    abstract = "Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.",
    keywords = "DNA detection, Environmental DNA, Species detection, Species-specific primers.",
    author = "Anna MACDONALD and Mark LINTERMANS and Stephen SARRE",
    year = "2014",
    doi = "10.1007/s12686-014-0196-6",
    language = "English",
    volume = "6",
    pages = "551--553",
    journal = "Conservation Genetics Resoures",
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    }

    Primers for detection of Macquarie perch from environmental and trace DNA samples. / MACDONALD, Anna; LINTERMANS, Mark; SARRE, Stephen.

    In: Conservation Genetics Resources, Vol. 6, No. 3, 2014, p. 551-553.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Primers for detection of Macquarie perch from environmental and trace DNA samples

    AU - MACDONALD, Anna

    AU - LINTERMANS, Mark

    AU - SARRE, Stephen

    PY - 2014

    Y1 - 2014

    N2 - Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.

    AB - Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.

    KW - DNA detection

    KW - Environmental DNA

    KW - Species detection

    KW - Species-specific primers.

    U2 - 10.1007/s12686-014-0196-6

    DO - 10.1007/s12686-014-0196-6

    M3 - Article

    VL - 6

    SP - 551

    EP - 553

    JO - Conservation Genetics Resoures

    JF - Conservation Genetics Resoures

    SN - 1877-7252

    IS - 3

    ER -