Production of phosphatase and extracellular stalks as adaptations to phosphorus limitation in Didymosphenia geminata (Bacillariophyceae)

Jonathan Bray, Jon O'Brien, Jon Harding

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Didymosphenia geminata is a benthic bloom-forming diatom that is invasive in many temperate, oligotrophic freshwater ecosystems. D. geminata blooms are unusual, resulting from prolific basal stalk production stimulated by phosphorus limitation. The adaptive value of stalk production and bloom development is disputed. We examined blooms in relation to stalk biomass, biovolume and phosphatase activity. An austral summer survey of 15 sites within the Waitaki River of New Zealand compared reference communities (no detectable D. geminata), with those impacted by high and low D. geminata biomass. Sites were compared for differences in phosphatase location and activity using chromogenic substrates, community composition using morphological identifications, and overlying water and pore-water chemistry. Experimental microcosms subjected live proliferations to varied phosphate concentrations, and phosphatase rates and location were examined. Survey results identified phosphatase activity increased with D. geminata biomass, with lowest rates in reference communities. Pools of labile nutrients were detected in D. geminata mats, and in vitro hydrolysis rates were rapid in replete phosphoester conditions (~0.2 mmol l-1 h-1 cm-2 at 16°C), with activity concentrated on stalks. Our results suggest D. geminata bloom development is an adaptation to maximise supply of phosphate under chronic phosphorus limitation.
Original languageEnglish
Pages (from-to)51-63
Number of pages13
JournalHydrobiologia
Volume784
Issue number1
DOIs
Publication statusPublished - Jan 2017

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Bacillariophyceae
phosphatase
algal bloom
phosphorus
biomass
phosphates
hydrochemistry
phosphate
hydrolysis
freshwater ecosystem
rivers
water chemistry
microcosm
summer
nutrients
community composition
porewater
fitness
diatom
substrate

Cite this

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title = "Production of phosphatase and extracellular stalks as adaptations to phosphorus limitation in Didymosphenia geminata (Bacillariophyceae)",
abstract = "Didymosphenia geminata is a benthic bloom-forming diatom that is invasive in many temperate, oligotrophic freshwater ecosystems. D. geminata blooms are unusual, resulting from prolific basal stalk production stimulated by phosphorus limitation. The adaptive value of stalk production and bloom development is disputed. We examined blooms in relation to stalk biomass, biovolume and phosphatase activity. An austral summer survey of 15 sites within the Waitaki River of New Zealand compared reference communities (no detectable D. geminata), with those impacted by high and low D. geminata biomass. Sites were compared for differences in phosphatase location and activity using chromogenic substrates, community composition using morphological identifications, and overlying water and pore-water chemistry. Experimental microcosms subjected live proliferations to varied phosphate concentrations, and phosphatase rates and location were examined. Survey results identified phosphatase activity increased with D. geminata biomass, with lowest rates in reference communities. Pools of labile nutrients were detected in D. geminata mats, and in vitro hydrolysis rates were rapid in replete phosphoester conditions (~0.2 mmol l-1 h-1 cm-2 at 16°C), with activity concentrated on stalks. Our results suggest D. geminata bloom development is an adaptation to maximise supply of phosphate under chronic phosphorus limitation.",
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Production of phosphatase and extracellular stalks as adaptations to phosphorus limitation in Didymosphenia geminata (Bacillariophyceae). / Bray, Jonathan; O'Brien, Jon; Harding, Jon.

In: Hydrobiologia, Vol. 784, No. 1, 01.2017, p. 51-63.

Research output: Contribution to journalArticle

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AB - Didymosphenia geminata is a benthic bloom-forming diatom that is invasive in many temperate, oligotrophic freshwater ecosystems. D. geminata blooms are unusual, resulting from prolific basal stalk production stimulated by phosphorus limitation. The adaptive value of stalk production and bloom development is disputed. We examined blooms in relation to stalk biomass, biovolume and phosphatase activity. An austral summer survey of 15 sites within the Waitaki River of New Zealand compared reference communities (no detectable D. geminata), with those impacted by high and low D. geminata biomass. Sites were compared for differences in phosphatase location and activity using chromogenic substrates, community composition using morphological identifications, and overlying water and pore-water chemistry. Experimental microcosms subjected live proliferations to varied phosphate concentrations, and phosphatase rates and location were examined. Survey results identified phosphatase activity increased with D. geminata biomass, with lowest rates in reference communities. Pools of labile nutrients were detected in D. geminata mats, and in vitro hydrolysis rates were rapid in replete phosphoester conditions (~0.2 mmol l-1 h-1 cm-2 at 16°C), with activity concentrated on stalks. Our results suggest D. geminata bloom development is an adaptation to maximise supply of phosphate under chronic phosphorus limitation.

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