Propofol-imprinted membranes with potential applications in biosensors

Miruna Petcu, Peter N. Schaare, Christian J. Cook

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Herein we describe a new thin film imprinted polymer for propofol (active ingredient of a commonly used general intravenous anaesthetic) developed on a membrane support. Propofol binds to this polymer in 2min and can be removed and tested in 1min, giving a total assay time of 3min. The non-specific binding to the polymer is below the detection limit (0.1μg/ml) and the response rate is below the rate of metabolism of the anaesthetic. Tests performed with this polymer against propofol-spiked blood samples showed good linearity and specificity at clinically relevant concentrations of 1-10μg/ml and the working range for the system is 0.1-50μg/ml. The polymer is easily regenerated and can be re-used for subsequent testing (in blood up to 5000 cycles). These results suggest suitability for use in an on-line propofol detection system.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalAnalytica Chimica Acta
Volume504
Issue number1
DOIs
Publication statusPublished - 16 Feb 2004
Externally publishedYes

Fingerprint

Biosensing Techniques
Propofol
Biosensors
Membrane Potentials
Polymers
polymer
membrane
Membranes
anesthetic
Blood
blood
Intravenous Anesthetics
General Anesthetics
linearity
Metabolism
Anesthetics
Limit of Detection
Assays
metabolism
assay

Cite this

Petcu, Miruna ; Schaare, Peter N. ; Cook, Christian J. / Propofol-imprinted membranes with potential applications in biosensors. In: Analytica Chimica Acta. 2004 ; Vol. 504, No. 1. pp. 73-79.
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Propofol-imprinted membranes with potential applications in biosensors. / Petcu, Miruna; Schaare, Peter N.; Cook, Christian J.

In: Analytica Chimica Acta, Vol. 504, No. 1, 16.02.2004, p. 73-79.

Research output: Contribution to journalArticle

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