Propofol-imprinted membranes with potential applications in biosensors

Miruna Petcu; Peter N. Schaare; Christian J. Cook

doi:10.1016/S0003-2670(03)00673-1

Propofol-imprinted membranes with potential applications in biosensors

Miruna Petcu, Peter N. Schaare, Christian J. Cook

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Herein we describe a new thin film imprinted polymer for propofol (active ingredient of a commonly used general intravenous anaesthetic) developed on a membrane support. Propofol binds to this polymer in 2min and can be removed and tested in 1min, giving a total assay time of 3min. The non-specific binding to the polymer is below the detection limit (0.1μg/ml) and the response rate is below the rate of metabolism of the anaesthetic. Tests performed with this polymer against propofol-spiked blood samples showed good linearity and specificity at clinically relevant concentrations of 1-10μg/ml and the working range for the system is 0.1-50μg/ml. The polymer is easily regenerated and can be re-used for subsequent testing (in blood up to 5000 cycles). These results suggest suitability for use in an on-line propofol detection system.

Original language	English
Pages (from-to)	73-79
Number of pages	7
Journal	Analytica Chimica Acta
Volume	504
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(03)00673-1
Publication status	Published - 16 Feb 2004
Externally published	Yes

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title = "Propofol-imprinted membranes with potential applications in biosensors",

abstract = "Herein we describe a new thin film imprinted polymer for propofol (active ingredient of a commonly used general intravenous anaesthetic) developed on a membrane support. Propofol binds to this polymer in 2min and can be removed and tested in 1min, giving a total assay time of 3min. The non-specific binding to the polymer is below the detection limit (0.1μg/ml) and the response rate is below the rate of metabolism of the anaesthetic. Tests performed with this polymer against propofol-spiked blood samples showed good linearity and specificity at clinically relevant concentrations of 1-10μg/ml and the working range for the system is 0.1-50μg/ml. The polymer is easily regenerated and can be re-used for subsequent testing (in blood up to 5000 cycles). These results suggest suitability for use in an on-line propofol detection system.",

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AU - Schaare, Peter N.

AU - Cook, Christian J.

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AB - Herein we describe a new thin film imprinted polymer for propofol (active ingredient of a commonly used general intravenous anaesthetic) developed on a membrane support. Propofol binds to this polymer in 2min and can be removed and tested in 1min, giving a total assay time of 3min. The non-specific binding to the polymer is below the detection limit (0.1μg/ml) and the response rate is below the rate of metabolism of the anaesthetic. Tests performed with this polymer against propofol-spiked blood samples showed good linearity and specificity at clinically relevant concentrations of 1-10μg/ml and the working range for the system is 0.1-50μg/ml. The polymer is easily regenerated and can be re-used for subsequent testing (in blood up to 5000 cycles). These results suggest suitability for use in an on-line propofol detection system.

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Propofol-imprinted membranes with potential applications in biosensors

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