Proteomic and functional analysis of human sperm detergent resistant membranes

Brett Nixon, Lisa A. Mitchell, Amanda L. Anderson, Eileen A. Mclaughlin, Moira K. O'bryan, R. John Aitken

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Mammalian spermatozoa attain the ability to fertilize an oocyte as they negotiate the female reproductive tract. This acquisition of functional competence is preceded by an intricate cascade of biochemical and functional changes collectively known as "capacitation." Among the universal correlates of the capacitation process is a remarkable remodeling of the lipid and protein architecture of the sperm plasma membrane. While the mechanisms that underpin this dynamic reorganization remain enigmatic, emerging evidence has raised the prospect that it may be coordinated, in part, by specialized membrane microdomains, or rafts. In the present study we have demonstrated that human spermatozoa express recognized markers of membrane rafts. Further, upon depletion of membrane cholesterol through either physiological (capacitation) or pharmacological (methyl-β-cyclodextrin) intervention, these membrane rafts appear to undergo a polarized redistribution to the peri-acrosomal region of the sperm head. This finding encourages speculation that membrane rafts represent platforms for the organization of proteins involved in sperm-oocyte interactions. Support for this notion rests with the demonstration that membrane rafts isolated on the basis of their biochemical composition in the form of detergent resistant membranes (DRMs), possess the ability to adhere to homologous zona pellucidae. Furthermore a comprehensive proteomic analysis of the DRMs identified a number of proteins known for their affinity for the zona pellucida in addition to other candidates putatively involved in the mediation of downstream binding and/or fusion with the oolemma. Collectively these data afford novel insights into the subcellular localization and potential functions of membrane rafts in human spermatozoa.

Original languageEnglish
Pages (from-to)2651-2665
Number of pages15
JournalJournal of Cellular Physiology
Volume226
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

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Functional analysis
Detergents
Proteomics
Spermatozoa
Membranes
Zona Pellucida
Aptitude
Membrane Microdomains
Sperm-Ovum Interactions
Sperm Head
Proteins
Cyclodextrins
Membrane Potentials
Mental Competency
Oocytes
Cell membranes
Cholesterol
Cell Membrane
Organizations
Pharmacology

Cite this

Nixon, B., Mitchell, L. A., Anderson, A. L., Mclaughlin, E. A., O'bryan, M. K., & Aitken, R. J. (2011). Proteomic and functional analysis of human sperm detergent resistant membranes. Journal of Cellular Physiology, 226(10), 2651-2665. https://doi.org/10.1002/jcp.22615
Nixon, Brett ; Mitchell, Lisa A. ; Anderson, Amanda L. ; Mclaughlin, Eileen A. ; O'bryan, Moira K. ; Aitken, R. John. / Proteomic and functional analysis of human sperm detergent resistant membranes. In: Journal of Cellular Physiology. 2011 ; Vol. 226, No. 10. pp. 2651-2665.
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Nixon, B, Mitchell, LA, Anderson, AL, Mclaughlin, EA, O'bryan, MK & Aitken, RJ 2011, 'Proteomic and functional analysis of human sperm detergent resistant membranes', Journal of Cellular Physiology, vol. 226, no. 10, pp. 2651-2665. https://doi.org/10.1002/jcp.22615

Proteomic and functional analysis of human sperm detergent resistant membranes. / Nixon, Brett; Mitchell, Lisa A.; Anderson, Amanda L.; Mclaughlin, Eileen A.; O'bryan, Moira K.; Aitken, R. John.

In: Journal of Cellular Physiology, Vol. 226, No. 10, 10.2011, p. 2651-2665.

Research output: Contribution to journalArticle

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Nixon B, Mitchell LA, Anderson AL, Mclaughlin EA, O'bryan MK, Aitken RJ. Proteomic and functional analysis of human sperm detergent resistant membranes. Journal of Cellular Physiology. 2011 Oct;226(10):2651-2665. https://doi.org/10.1002/jcp.22615