TY - JOUR
T1 - QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source
AU - Linsell, Katherine
AU - Rahman, Muhammad S
AU - Taylor, Julian
AU - Davey, Rowena
AU - Gogel, Beverley
AU - Wallwork, Hugh
AU - Forrest, Kerrie
AU - Hayden, Matthew
AU - Taylor, Sharyn P.
AU - Oldach, Klaus
N1 - Funding Information:
This research was supported by the Grains Research and Development Corporation (GRDC) through a Grains Industry Research Scholarship to K.J. Linsell and for funding the original screening of the mapping population and field evaluations. The project was also supported by the South Australian Research and Development Institute (SARDI) and Molecular Plant Breeding Cooperative Research Centre (MPBCRC). The authors would like to thank K. Willsmore for MapManger QTX training, to the SARDI Nematology Group, especially D. Pounsett for assistance with culturing of the nematodes, to A. McKay (SARDI) and A. Cook (Minnipa Agricultural Centre) for coordinating the field trials, and to I. Riley at SARDI and K. Davies at the University of Adelaide for manuscript editing. Further thanks are to H. Eagles, University of Adelaide, for the information on pedigrees and COP value.
PY - 2014/6
Y1 - 2014/6
N2 - Key message: A whole genome average interval mapping approach identified eight QTL associated with P. thornei resistance in a DH population from a cross between the synthetic-derived wheat Sokoll and cultivar Krichauff. Pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. Genotypic selection using molecular markers closely linked to Pratylenchus resistance genes will accelerate the development of new resistant cultivars by reducing the need for laborious and expensive resistance phenotyping. A doubled haploid wheat population (150 lines) from a cross between the synthetic-derived cultivar Sokoll (P. thornei resistant) and cultivar Krichauff (P. thornei moderately susceptible) was used to identify quantitative trait loci (QTL) associated with P. thornei resistance. The resistance identified in the glasshouse was validated in a field trial. A genetic map was constructed using Diversity Array Technology and the QTL regions identified were further targeted with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. Six significant and two suggestive P. thornei resistance QTL were detected using a whole genome average interval mapping approach. Three QTL were identified on chromosome 2B, two on chromosome 6D, and a single QTL on each of chromosomes 2A, 2D and 5D. The QTL on chromosomes 2BS and 6DS mapped to locations previously identified to be associated with Pratylenchus resistance. Together, the QTL on 2B (QRlnt.sk-2B.1-2B.3) and 6D (QRlnt.sk-6D.1 and 6D.2) explained 30 and 48 % of the genotypic variation, respectively. Flanking PCR-based markers based on SSRs and SNPs were developed for the major QTL on 2B and 6D and provide a cost-effective high-throughput tool for marker-assisted breeding of wheat with improved P. thornei resistance.
AB - Key message: A whole genome average interval mapping approach identified eight QTL associated with P. thornei resistance in a DH population from a cross between the synthetic-derived wheat Sokoll and cultivar Krichauff. Pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. Genotypic selection using molecular markers closely linked to Pratylenchus resistance genes will accelerate the development of new resistant cultivars by reducing the need for laborious and expensive resistance phenotyping. A doubled haploid wheat population (150 lines) from a cross between the synthetic-derived cultivar Sokoll (P. thornei resistant) and cultivar Krichauff (P. thornei moderately susceptible) was used to identify quantitative trait loci (QTL) associated with P. thornei resistance. The resistance identified in the glasshouse was validated in a field trial. A genetic map was constructed using Diversity Array Technology and the QTL regions identified were further targeted with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. Six significant and two suggestive P. thornei resistance QTL were detected using a whole genome average interval mapping approach. Three QTL were identified on chromosome 2B, two on chromosome 6D, and a single QTL on each of chromosomes 2A, 2D and 5D. The QTL on chromosomes 2BS and 6DS mapped to locations previously identified to be associated with Pratylenchus resistance. Together, the QTL on 2B (QRlnt.sk-2B.1-2B.3) and 6D (QRlnt.sk-6D.1 and 6D.2) explained 30 and 48 % of the genotypic variation, respectively. Flanking PCR-based markers based on SSRs and SNPs were developed for the major QTL on 2B and 6D and provide a cost-effective high-throughput tool for marker-assisted breeding of wheat with improved P. thornei resistance.
UR - http://www.scopus.com/inward/record.url?scp=84901614631&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/qtl-resistance-root-lesion-nematode-pratylenchus-thornei-synthetic-hexaploid-wheat-source
U2 - 10.1007/s00122-014-2308-9
DO - 10.1007/s00122-014-2308-9
M3 - Article
SN - 0040-5752
VL - 127
SP - 1409
EP - 1421
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 6
ER -