QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source

Katherine Linsell, Muhammad S Rahman, Julian Taylor, Rowena Davey, Beverley Gogel, Hugh Wallwork, Kerrie Forrest, Matthew Hayden, Sharyn P. Taylor, Klaus Oldach

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Key message: A whole genome average interval mapping approach identified eight QTL associated with P. thornei resistance in a DH population from a cross between the synthetic-derived wheat Sokoll and cultivar Krichauff. Pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. Genotypic selection using molecular markers closely linked to Pratylenchus resistance genes will accelerate the development of new resistant cultivars by reducing the need for laborious and expensive resistance phenotyping. A doubled haploid wheat population (150 lines) from a cross between the synthetic-derived cultivar Sokoll (P. thornei resistant) and cultivar Krichauff (P. thornei moderately susceptible) was used to identify quantitative trait loci (QTL) associated with P. thornei resistance. The resistance identified in the glasshouse was validated in a field trial. A genetic map was constructed using Diversity Array Technology and the QTL regions identified were further targeted with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. Six significant and two suggestive P. thornei resistance QTL were detected using a whole genome average interval mapping approach. Three QTL were identified on chromosome 2B, two on chromosome 6D, and a single QTL on each of chromosomes 2A, 2D and 5D. The QTL on chromosomes 2BS and 6DS mapped to locations previously identified to be associated with Pratylenchus resistance. Together, the QTL on 2B (QRlnt.sk-2B.1-2B.3) and 6D (QRlnt.sk-6D.1 and 6D.2) explained 30 and 48 % of the genotypic variation, respectively. Flanking PCR-based markers based on SSRs and SNPs were developed for the major QTL on 2B and 6D and provide a cost-effective high-throughput tool for marker-assisted breeding of wheat with improved P. thornei resistance.
Original languageEnglish
Pages (from-to)1409-1421
Number of pages13
JournalTheoretical And Applied Genetics
Volume127
Issue number6
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

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Pratylenchus thornei
root lesion nematodes
Quantitative Trait Loci
hexaploidy
Triticum
quantitative trait loci
wheat
Chromosomes
chromosomes
Pratylenchus
cultivars
Single Nucleotide Polymorphism
Genome
genome
Haploidy
doubled haploids
lesions (animal)
Microsatellite Repeats
single nucleotide polymorphism
Population

Cite this

Linsell, K., Rahman, M. S., Taylor, J., Davey, R., Gogel, B., Wallwork, H., ... Oldach, K. (2014). QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source. Theoretical And Applied Genetics, 127(6), 1409-1421. https://doi.org/10.1007/s00122-014-2308-9
Linsell, Katherine ; Rahman, Muhammad S ; Taylor, Julian ; Davey, Rowena ; Gogel, Beverley ; Wallwork, Hugh ; Forrest, Kerrie ; Hayden, Matthew ; Taylor, Sharyn P. ; Oldach, Klaus. / QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source. In: Theoretical And Applied Genetics. 2014 ; Vol. 127, No. 6. pp. 1409-1421.
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Linsell, K, Rahman, MS, Taylor, J, Davey, R, Gogel, B, Wallwork, H, Forrest, K, Hayden, M, Taylor, SP & Oldach, K 2014, 'QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source', Theoretical And Applied Genetics, vol. 127, no. 6, pp. 1409-1421. https://doi.org/10.1007/s00122-014-2308-9

QTL for resistance to root lesion nematode (Pratylenchus thornei) from a synthetic hexaploid wheat source. / Linsell, Katherine; Rahman, Muhammad S; Taylor, Julian; Davey, Rowena; Gogel, Beverley; Wallwork, Hugh; Forrest, Kerrie; Hayden, Matthew; Taylor, Sharyn P. ; Oldach, Klaus.

In: Theoretical And Applied Genetics, Vol. 127, No. 6, 06.2014, p. 1409-1421.

Research output: Contribution to journalArticle

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AU - Linsell, Katherine

AU - Rahman, Muhammad S

AU - Taylor, Julian

AU - Davey, Rowena

AU - Gogel, Beverley

AU - Wallwork, Hugh

AU - Forrest, Kerrie

AU - Hayden, Matthew

AU - Taylor, Sharyn P.

AU - Oldach, Klaus

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AB - Key message: A whole genome average interval mapping approach identified eight QTL associated with P. thornei resistance in a DH population from a cross between the synthetic-derived wheat Sokoll and cultivar Krichauff. Pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. Genotypic selection using molecular markers closely linked to Pratylenchus resistance genes will accelerate the development of new resistant cultivars by reducing the need for laborious and expensive resistance phenotyping. A doubled haploid wheat population (150 lines) from a cross between the synthetic-derived cultivar Sokoll (P. thornei resistant) and cultivar Krichauff (P. thornei moderately susceptible) was used to identify quantitative trait loci (QTL) associated with P. thornei resistance. The resistance identified in the glasshouse was validated in a field trial. A genetic map was constructed using Diversity Array Technology and the QTL regions identified were further targeted with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. Six significant and two suggestive P. thornei resistance QTL were detected using a whole genome average interval mapping approach. Three QTL were identified on chromosome 2B, two on chromosome 6D, and a single QTL on each of chromosomes 2A, 2D and 5D. The QTL on chromosomes 2BS and 6DS mapped to locations previously identified to be associated with Pratylenchus resistance. Together, the QTL on 2B (QRlnt.sk-2B.1-2B.3) and 6D (QRlnt.sk-6D.1 and 6D.2) explained 30 and 48 % of the genotypic variation, respectively. Flanking PCR-based markers based on SSRs and SNPs were developed for the major QTL on 2B and 6D and provide a cost-effective high-throughput tool for marker-assisted breeding of wheat with improved P. thornei resistance.

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