Quality control technique to reduce the variability of longitudinal measurement of hemoglobin mass

Clare Gough, Ken Sharpe, Michael Ashenden, Judith Anson, Philo SAUNDERS, Laura Garvican, Darrell L. Bonetti, Christopher Gore, Nicole Prommer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The sensitivity of the athlete blood passport to detect blood doping may be improved by the inclusion of total hemoglobin mass (Hbmass), but the comparability of Hbmass from different laboratories is unknown. To optimize detection sensitivity, the analytical variability associated with Hbmass measurement must be minimized. The aim of this study was to investigate the efficacy of using quality controls to minimize the variation in Hbmass between laboratories. Three simulated laboratories were set up in one location. Nine participants completed three carbon monoxide (CO) re-breathing tests in each laboratory. One participant completed two CO re-breathing tests in each laboratory. Simultaneously, quality controls containing Low (1–3%) and High (8–11%) concentrations of percent carboxyhemoglobin (%HbCO) were measured to compare hemoximeters in each laboratory. Linear mixed modeling was used to estimate the within-subject variation in Hbmass, expressed as the coefficient of variation, and to estimate the effect of different laboratories. The analytic variation of Hbmass was 2.4% when tests were conducted in different laboratories, which reduced to 1.6% when the model accounted for between-laboratory differences. Adjustment of Hbmass values using quality controls achieved a comparable analytic variation of 1.7%. The majority of between-laboratory variation in Hbmass originated from the difference between hemoximeters, which could be eliminated using appropriate quality controls.
Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalScandinavian Journal of Medicine and Science in Sports
Volume21
Issue number6
DOIs
Publication statusPublished - 2011

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Quality Control
Hemoglobins
Carbon Monoxide
Respiration
Doping in Sports
Carboxyhemoglobin
Athletes

Cite this

Gough, Clare ; Sharpe, Ken ; Ashenden, Michael ; Anson, Judith ; SAUNDERS, Philo ; Garvican, Laura ; Bonetti, Darrell L. ; Gore, Christopher ; Prommer, Nicole. / Quality control technique to reduce the variability of longitudinal measurement of hemoglobin mass. In: Scandinavian Journal of Medicine and Science in Sports. 2011 ; Vol. 21, No. 6. pp. 365-371.
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Gough, C, Sharpe, K, Ashenden, M, Anson, J, SAUNDERS, P, Garvican, L, Bonetti, DL, Gore, C & Prommer, N 2011, 'Quality control technique to reduce the variability of longitudinal measurement of hemoglobin mass', Scandinavian Journal of Medicine and Science in Sports, vol. 21, no. 6, pp. 365-371. https://doi.org/10.1111/J.1600-0838.2011.01316.X

Quality control technique to reduce the variability of longitudinal measurement of hemoglobin mass. / Gough, Clare; Sharpe, Ken; Ashenden, Michael; Anson, Judith; SAUNDERS, Philo; Garvican, Laura; Bonetti, Darrell L.; Gore, Christopher; Prommer, Nicole.

In: Scandinavian Journal of Medicine and Science in Sports, Vol. 21, No. 6, 2011, p. 365-371.

Research output: Contribution to journalArticle

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AU - Gough, Clare

AU - Sharpe, Ken

AU - Ashenden, Michael

AU - Anson, Judith

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AU - Garvican, Laura

AU - Bonetti, Darrell L.

AU - Gore, Christopher

AU - Prommer, Nicole

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