Rapid generation of dityrosine cross-linked Aβ oligomers via Cu-redox cycling

Adam P Gunn, Blaine R Roberts, Ashley I Bush

Research output: A Conference proceeding or a Chapter in BookOther chapter contribution

7 Citations (Scopus)

Abstract

There is a great interest in the role of free radicals and oxidative stress in Alzheimer's disease and for the role of transition metals in the generation of oligomers of Aβ peptides. In the literature, there are a multitude of varying methods that can be used to create soluble oligomers of Aβ, however, the processes that create these oligomers are often stochastic by nature and thus reproducibility is an issue. Here we report a simple and reproducible method for the production of radically derived dityrosine cross-linked oligomers of Aβ, through reaction with copper and ascorbic acid.

Original languageEnglish
Title of host publicationAmyloid Proteins
Subtitle of host publicationMethods and Protocols
EditorsEinar M. Sigurdsson, Miguel Calero, María Gasset
Place of PublicationUnited States
PublisherSpringer
Chapter1
Pages3-10
Number of pages8
Volume849
ISBN (Electronic)9781617795510
ISBN (Print)9781617795503
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

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  • Cite this

    Gunn, A. P., Roberts, B. R., & Bush, A. I. (2012). Rapid generation of dityrosine cross-linked Aβ oligomers via Cu-redox cycling. In E. M. Sigurdsson, M. Calero, & M. Gasset (Eds.), Amyloid Proteins: Methods and Protocols (Vol. 849, pp. 3-10). (Methods in molecular biology (Clifton, N.J.)). Springer. https://doi.org/10.1007/978-1-61779-551-0_1