Reassignment of chicken W chromosome sequences to the Z chromosome by fluorescence in situ hybridization (FISH)

Rami Stiglec, Tariq Ezaz, Jenny Graves

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

There is much interest in the gene content of the small heterochromatic W chromosome of the chicken, on the supposition that it may contain sex-determining genes. A considerable region in the chicken genome has been assigned to the W chromosome on the basis of its repetitive sequences. Using fluorescent in situ hybridization (FISH) we localized five Bacterial Artificial Chromosomes (BACs) onto female chicken metaphase spreads. We physically mapped these BACs to the Z chromosome. The chicken genome database, however, assigned all five BACs to the W chromosome. Our results demonstrate that the 17 genes on these BACs are Z-specific, and points to the inadequacy of assigning regions of the genome based exclusively on repetitive sequences
Original languageEnglish
Pages (from-to)132-134
Number of pages3
JournalCytogenetics and Cell Genetics
Volume116
Issue number1-2
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Bacterial Artificial Chromosomes
Fluorescence In Situ Hybridization
Chickens
Chromosomes
Nucleic Acid Repetitive Sequences
Genome
Genes
Metaphase
Databases

Cite this

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abstract = "There is much interest in the gene content of the small heterochromatic W chromosome of the chicken, on the supposition that it may contain sex-determining genes. A considerable region in the chicken genome has been assigned to the W chromosome on the basis of its repetitive sequences. Using fluorescent in situ hybridization (FISH) we localized five Bacterial Artificial Chromosomes (BACs) onto female chicken metaphase spreads. We physically mapped these BACs to the Z chromosome. The chicken genome database, however, assigned all five BACs to the W chromosome. Our results demonstrate that the 17 genes on these BACs are Z-specific, and points to the inadequacy of assigning regions of the genome based exclusively on repetitive sequences",
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Reassignment of chicken W chromosome sequences to the Z chromosome by fluorescence in situ hybridization (FISH). / Stiglec, Rami; Ezaz, Tariq; Graves, Jenny.

In: Cytogenetics and Cell Genetics, Vol. 116, No. 1-2, 2007, p. 132-134.

Research output: Contribution to journalArticle

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