Real-Time Measurements of Corticosteroids In Conscious Animals Using An Antibody-Based Electrode

Christian J. Cook

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

An electrochemical immunosensor for real-time determination of corticosteroids, a group of steroidal hormones, is reported. The sensor measures competitive binding of endogenous corticosteroid and a corticosteroid-peroxidase conjugate with antibodies, immobilized on a platinum electrode, by monitoring of peroxidase activity. The electrode is encased within a dialysate membrane, which separates the electrode environment from the sampled fluid, allowing corticosteroid to equilibrate across the membrane. This permits measurements to be made in vivo. The small size of the probe (350 μm D) allows implantation into tissue or circulatory systems. The electrode was viable for 200 to 400 sequential measurements and at least 48 h in vivo. Detection sensitivity is 0.2 to 0.6 μg/100 ml of cortisol or corticosterone. The results suggest the possibility of monitoring hormones in real-time within the blood or organ systems of conscious animals.

Original languageEnglish
Pages (from-to)467-471
Number of pages5
JournalNature Biotechnology
Volume15
Issue number5
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Time measurement
Antibodies
Adrenal Cortex Hormones
Electrodes
Animals
Hormones
Peroxidase
Cortisol
Immobilized Antibodies
Membranes
Immunosensors
Competitive Binding
Monitoring
Dialysis Solutions
Corticosterone
Cardiovascular System
Platinum
Hydrocortisone
Blood
Tissue

Cite this

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Real-Time Measurements of Corticosteroids In Conscious Animals Using An Antibody-Based Electrode. / Cook, Christian J.

In: Nature Biotechnology, Vol. 15, No. 5, 1997, p. 467-471.

Research output: Contribution to journalArticle

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