Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review

Ahmed Abdulrahman, Ashraf Ghanem

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

The wide spread of infectious diseases have provoked the scientists to develop new types of vaccines. Among the different types of vaccines, the recently discovered plasmid DNA vaccines, have gained tremendous attentions in the last few decades as a modern approach of vaccination. The scientific interest in plasmid DNA vaccines is attributed to their prominent efficacy as they trigger not only the cellular immune response but also the humoral immune responses. Moreover, pDNA vaccines are easily to be stored, shipped and produced. However, the purification of the pDNA vaccines is a crucial step in their production and administration, which is usually conducted by different chromatographic techniques. This review summarizes the most recent chromatographic purification methods provided in the literature during the last five years following our last review in 2013, including affinity chromatography, hydrophobic interaction chromatography, ion exchange chromatography, multimodal chromatography, sample displacement chromatography and miscellaneous chromatographic methods.

Original languageEnglish
Pages (from-to)41-57
Number of pages17
JournalAnalytica Chimica Acta
Volume1025
DOIs
Publication statusPublished - 26 Sep 2018

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Gene therapy
DNA Vaccines
vaccine
plasmid
Genetic Therapy
Purification
purification
Plasmids
Vaccines
Chromatography
chromatography
DNA
gene
immune response
Affinity chromatography
Ion Exchange Chromatography
Humoral Immunity
Hydrophobic and Hydrophilic Interactions
Affinity Chromatography
Cellular Immunity

Cite this

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abstract = "The wide spread of infectious diseases have provoked the scientists to develop new types of vaccines. Among the different types of vaccines, the recently discovered plasmid DNA vaccines, have gained tremendous attentions in the last few decades as a modern approach of vaccination. The scientific interest in plasmid DNA vaccines is attributed to their prominent efficacy as they trigger not only the cellular immune response but also the humoral immune responses. Moreover, pDNA vaccines are easily to be stored, shipped and produced. However, the purification of the pDNA vaccines is a crucial step in their production and administration, which is usually conducted by different chromatographic techniques. This review summarizes the most recent chromatographic purification methods provided in the literature during the last five years following our last review in 2013, including affinity chromatography, hydrophobic interaction chromatography, ion exchange chromatography, multimodal chromatography, sample displacement chromatography and miscellaneous chromatographic methods.",
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Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review. / Abdulrahman, Ahmed; Ghanem, Ashraf.

In: Analytica Chimica Acta, Vol. 1025, 26.09.2018, p. 41-57.

Research output: Contribution to journalReview article

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AU - Ghanem, Ashraf

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