Relative abundance of Mycobacterium in ovine Johne's disease

Andy O Leu; Paul Pavli; David Gordon; Jeff Cave; Jacek M Gowzdz; Nick Linden; Grant Rawlin; Gwen E. Allison; Claire O'Brien

doi:10.1071/MA15010

Relative abundance of Mycobacterium in ovine Johne's disease

Andy O Leu, Paul Pavli, David Gordon, Jeff Cave, Jacek M Gowzdz, Nick Linden, Grant Rawlin, Gwen E. Allison, Claire O'Brien

Research output: Contribution to journal › Article › peer-review

Abstract

No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johne’s disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Microbiology Australia
Volume	36
Issue number	1
DOIs	https://doi.org/10.1071/MA15010
Publication status	Published - 6 Mar 2015
Externally published	Yes

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abstract = "No study has determined what proportion of the total microbiota comprises the genus Mycobacterium in ovine Johne{\textquoteright}s disease (OJD) tissues. We aimed to assess the relative abundance of Mycobacterium in the ileocaecal lymph node, involved and uninvolved ileal mucosa from sheep with and without OJD, using three extraction methods. Eight sheep, four with and four without OJD, were recruited. Pyrosequencing of the 16Sr RNA gene amplicons for all samples revealed that Mycobacterium represented between 0-92% (average 38%) of the total microbiota of samples from sheep with OJD, and 0-85% (average 13%) of sheep without OJD. Only sheep with OJD had samples that were positive for the IS900 (MAP) element. Mycobacterial strains other than MAP may provide competitive exclusion of MAP and should be further investigated.",

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AU - Gowzdz, Jacek M

AU - Linden, Nick

AU - Rawlin, Grant

AU - Allison, Gwen E.

AU - O'Brien, Claire

PY - 2015/3/6

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Relative abundance of Mycobacterium in ovine Johne's disease

Abstract

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