Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells

R Ghildyal, J Mills, M Murray, N Vardaxis, J Meanger

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Little is known about the functions of the matrix (M) protein of respiratory syncytial virus (RSV). By analogy with other negative-strand RNA viruses, the M protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. In this study, localization of the RSV M protein in infected cells and its association with the RSV nucleocapsid complex was investigated. RSV-infected cells were shown to contain characteristic cytoplasmic inclusions. Further analysis showed that these inclusions were localization sites of the M protein as well as the N, P, L and M2-1 proteins described previously. The M protein co-purified with viral ribonucleoproteins (RNPs) from RSV-infected cells. The transcriptase activity of purified RNPs was enhanced by treatment with antibodies to the M protein in a dose-dependent manner. These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription.

Original languageEnglish
Pages (from-to)753-757
Number of pages5
JournalJournal of General Virology
Volume83
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Nucleocapsid
Respiratory Syncytial Viruses
Proteins
Ribonucleoproteins
RNA Viruses
Inclusion Bodies
Product Packaging
DNA-Directed RNA Polymerases
Virion
Viruses
Antibodies

Cite this

Ghildyal, R ; Mills, J ; Murray, M ; Vardaxis, N ; Meanger, J. / Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells. In: Journal of General Virology. 2002 ; Vol. 83, No. 4. pp. 753-757.
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abstract = "Little is known about the functions of the matrix (M) protein of respiratory syncytial virus (RSV). By analogy with other negative-strand RNA viruses, the M protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. In this study, localization of the RSV M protein in infected cells and its association with the RSV nucleocapsid complex was investigated. RSV-infected cells were shown to contain characteristic cytoplasmic inclusions. Further analysis showed that these inclusions were localization sites of the M protein as well as the N, P, L and M2-1 proteins described previously. The M protein co-purified with viral ribonucleoproteins (RNPs) from RSV-infected cells. The transcriptase activity of purified RNPs was enhanced by treatment with antibodies to the M protein in a dose-dependent manner. These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription.",
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Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells. / Ghildyal, R; Mills, J; Murray, M; Vardaxis, N; Meanger, J.

In: Journal of General Virology, Vol. 83, No. 4, 2002, p. 753-757.

Research output: Contribution to journalArticle

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T1 - Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells

AU - Ghildyal, R

AU - Mills, J

AU - Murray, M

AU - Vardaxis, N

AU - Meanger, J

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AB - Little is known about the functions of the matrix (M) protein of respiratory syncytial virus (RSV). By analogy with other negative-strand RNA viruses, the M protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. In this study, localization of the RSV M protein in infected cells and its association with the RSV nucleocapsid complex was investigated. RSV-infected cells were shown to contain characteristic cytoplasmic inclusions. Further analysis showed that these inclusions were localization sites of the M protein as well as the N, P, L and M2-1 proteins described previously. The M protein co-purified with viral ribonucleoproteins (RNPs) from RSV-infected cells. The transcriptase activity of purified RNPs was enhanced by treatment with antibodies to the M protein in a dose-dependent manner. These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription.

KW - Cell Line

KW - Humans

KW - Nucleocapsid

KW - Respiratory Syncytial Virus, Human

KW - Transcription, Genetic

KW - Viral Matrix Proteins

KW - Viral Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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