Restriction site-associated DNA sequencing (RAD-seq) reveals an extraordinary number of transitions among gecko sex-determining systems

Tony Gamble, Jessi Coryell, Tariq EZAZ, Joshua Lynch, Daniel Scantlebury, David Zarkower

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    Sex chromosomes have evolved many times in animals and studying these replicate evolutionary "experiments" can help broaden our understanding of the general forces driving the origin and evolution of sex chromosomes. However this plan of study has been hindered by the inability to identify the sex chromosome systems in the large number of species with cryptic, homomorphic sex chromosomes. Restriction site-associated DNA sequencing (RAD-seq) is a critical enabling technology that can identify the sex chromosome systems in many species where traditional cytogenetic methods have failed. Using newly generated RAD-seq data from 12 gecko species, along with data from the literature, we reinterpret the evolution of sex-determining systems in lizards and snakes and test the hypothesis that sex chromosomes can routinely act as evolutionary traps. We uncovered between 17 and 25 transitions among gecko sex-determining systems. This is approximately one-half to two-thirds of the total number of transitions observed among all lizards and snakes. We find support for the hypothesis that sex chromosome systems can readily become trap-like and show that adding even a small number of species from understudied clades can greatly enhance hypothesis testing in a model-based phylogenetic framework. RAD-seq will undoubtedly prove useful in evaluating other species for male or female heterogamety, particularly the majority of fish, amphibian, and reptile species that lack visibly heteromorphic sex chromosomes, and will significantly accelerate the pace of biological discovery.
    Original languageEnglish
    Pages (from-to)1296-1309
    Number of pages14
    JournalMolecular Biology and Evolution
    Volume32
    Issue number5
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Sex Chromosomes
    Lizards
    Gekkonidae
    sex chromosomes
    DNA Sequence Analysis
    chromosome
    sequence analysis
    DNA
    gender
    Snakes
    snake
    lizard
    snakes
    lizards
    traps
    Reptiles
    hypothesis testing
    cytogenetics
    Amphibians
    reptile

    Cite this

    Gamble, Tony ; Coryell, Jessi ; EZAZ, Tariq ; Lynch, Joshua ; Scantlebury, Daniel ; Zarkower, David. / Restriction site-associated DNA sequencing (RAD-seq) reveals an extraordinary number of transitions among gecko sex-determining systems. In: Molecular Biology and Evolution. 2015 ; Vol. 32, No. 5. pp. 1296-1309.
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    abstract = "Sex chromosomes have evolved many times in animals and studying these replicate evolutionary {"}experiments{"} can help broaden our understanding of the general forces driving the origin and evolution of sex chromosomes. However this plan of study has been hindered by the inability to identify the sex chromosome systems in the large number of species with cryptic, homomorphic sex chromosomes. Restriction site-associated DNA sequencing (RAD-seq) is a critical enabling technology that can identify the sex chromosome systems in many species where traditional cytogenetic methods have failed. Using newly generated RAD-seq data from 12 gecko species, along with data from the literature, we reinterpret the evolution of sex-determining systems in lizards and snakes and test the hypothesis that sex chromosomes can routinely act as evolutionary traps. We uncovered between 17 and 25 transitions among gecko sex-determining systems. This is approximately one-half to two-thirds of the total number of transitions observed among all lizards and snakes. We find support for the hypothesis that sex chromosome systems can readily become trap-like and show that adding even a small number of species from understudied clades can greatly enhance hypothesis testing in a model-based phylogenetic framework. RAD-seq will undoubtedly prove useful in evaluating other species for male or female heterogamety, particularly the majority of fish, amphibian, and reptile species that lack visibly heteromorphic sex chromosomes, and will significantly accelerate the pace of biological discovery.",
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    Restriction site-associated DNA sequencing (RAD-seq) reveals an extraordinary number of transitions among gecko sex-determining systems. / Gamble, Tony; Coryell, Jessi; EZAZ, Tariq; Lynch, Joshua; Scantlebury, Daniel; Zarkower, David.

    In: Molecular Biology and Evolution, Vol. 32, No. 5, 2015, p. 1296-1309.

    Research output: Contribution to journalArticle

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