Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium

Howard Donninger, Richard Glashoff, Hans-Michael Haitchi, James A Syce, Reena Ghildyal, Estrelita van Rensburg, Philip G Bardin

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.

Original languageEnglish
Pages (from-to)1809-17
Number of pages9
JournalJournal of Infectious Diseases
Volume187
Issue number11
DOIs
Publication statusPublished - 1 Jun 2003
Externally publishedYes

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CXC Chemokines
Rhinovirus
Neutrophils
Epithelium
Infection
Epithelial Cells
peptide 78
Common Cold
Messenger RNA
Virus Diseases
Nose
Chemokines
Reverse Transcription
Proteins
Asthma
Enzyme-Linked Immunosorbent Assay
Pathology
Viruses
Inflammation

Cite this

Donninger, Howard ; Glashoff, Richard ; Haitchi, Hans-Michael ; Syce, James A ; Ghildyal, Reena ; van Rensburg, Estrelita ; Bardin, Philip G. / Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium. In: Journal of Infectious Diseases. 2003 ; Vol. 187, No. 11. pp. 1809-17.
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abstract = "Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.",
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Donninger, H, Glashoff, R, Haitchi, H-M, Syce, JA, Ghildyal, R, van Rensburg, E & Bardin, PG 2003, 'Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium', Journal of Infectious Diseases, vol. 187, no. 11, pp. 1809-17. https://doi.org/10.1086/375246

Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium. / Donninger, Howard; Glashoff, Richard; Haitchi, Hans-Michael; Syce, James A; Ghildyal, Reena; van Rensburg, Estrelita; Bardin, Philip G.

In: Journal of Infectious Diseases, Vol. 187, No. 11, 01.06.2003, p. 1809-17.

Research output: Contribution to journalArticle

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T1 - Rhinovirus induction of the CXC chemokine epithelial-neutrophil activating peptide-78 in bronchial epithelium

AU - Donninger, Howard

AU - Glashoff, Richard

AU - Haitchi, Hans-Michael

AU - Syce, James A

AU - Ghildyal, Reena

AU - van Rensburg, Estrelita

AU - Bardin, Philip G

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N2 - Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.

AB - Epithelial-neutrophil activating peptide-78 (ENA-78) induces neutrophil migration, an early response to viral infection. Rhinovirus serotype 16 (RV16) was used to infect primary bronchial epithelial cells and a cell line (BEAS-2B). Release of ENA-78 protein was measured by enzyme-linked immunosorbent assay, ENA-78 mRNA production was quantified by reverse-transcription polymerase chain reaction, and ENA-78 promoter activity was assessed by use of a promoter construct. After infection with RV16, ENA-78 protein and mRNA increased significantly, and RV16 induced 3-fold increases in ENA-78 gene transcription. Nasal ENA-78 measured in patients with asthma with and without RV infection was more elevated in patients with RV infection present. Our study demonstrates that ENA-78 is produced in bronchial epithelial cells in response to RV16 infection. With other chemokines, it may be an important initiator of neutrophil airway inflammation during RV common colds and thus may play a role in the development of virus-associated airway pathologies.

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KW - Chemokine CXCL5

KW - Chemokines, CXC

KW - Epithelium

KW - Female

KW - Gene Expression Regulation

KW - Humans

KW - Interleukin-8

KW - Male

KW - Picornaviridae Infections

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Rhinovirus

KW - Time Factors

KW - Transcription, Genetic

KW - Virus Replication

KW - Journal Article

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