RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis

Jessie M. Sutherland, Alexander P. Sobinoff, Barbara A. Fraser, Kate A. Redgrove, Nicole A. Siddall, Peter Koopman, Gary R. Hime, Eileen A. McLaughlin

Research output: Contribution to journalArticle

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Abstract

RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalization, identified differential expression and localization of important regulators of transcription, translation, RNA processing, and spermatogenesis. Specifically, the testis-expressed transcription factor, Tbx1, and the piRNA regulator of gamete development, Piwil1, were both found to be targeted for translational repression by MSI2. This study provides key evidence to support a fundamental role for MSI2 in post-transcriptional regulation during male gamete development.

Original languageEnglish
Pages (from-to)3262-3273
Number of pages12
JournalJournal of Cellular Physiology
Volume233
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Spermatogenesis
Gene expression
Germ Cells
Cells
Cell death
Transcription
Microarrays
Small Interfering RNA
Nuclear RNA
Transcription Factors
Genes
RNA
Microarray Analysis
Fertility
Spermatozoa
Testis
Cell Cycle
Cell Death
Up-Regulation

Cite this

Sutherland, J. M., Sobinoff, A. P., Fraser, B. A., Redgrove, K. A., Siddall, N. A., Koopman, P., ... McLaughlin, E. A. (2018). RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. Journal of Cellular Physiology, 233(4), 3262-3273. https://doi.org/10.1002/jcp.26168
Sutherland, Jessie M. ; Sobinoff, Alexander P. ; Fraser, Barbara A. ; Redgrove, Kate A. ; Siddall, Nicole A. ; Koopman, Peter ; Hime, Gary R. ; McLaughlin, Eileen A. / RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. In: Journal of Cellular Physiology. 2018 ; Vol. 233, No. 4. pp. 3262-3273.
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Sutherland, JM, Sobinoff, AP, Fraser, BA, Redgrove, KA, Siddall, NA, Koopman, P, Hime, GR & McLaughlin, EA 2018, 'RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis', Journal of Cellular Physiology, vol. 233, no. 4, pp. 3262-3273. https://doi.org/10.1002/jcp.26168

RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. / Sutherland, Jessie M.; Sobinoff, Alexander P.; Fraser, Barbara A.; Redgrove, Kate A.; Siddall, Nicole A.; Koopman, Peter; Hime, Gary R.; McLaughlin, Eileen A.

In: Journal of Cellular Physiology, Vol. 233, No. 4, 01.04.2018, p. 3262-3273.

Research output: Contribution to journalArticle

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Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Siddall NA, Koopman P et al. RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. Journal of Cellular Physiology. 2018 Apr 1;233(4):3262-3273. https://doi.org/10.1002/jcp.26168