RNA-dependent RNA polymerases of both virulent and benign Rabbit caliciviruses induce striking rearrangement of Golgi membranes

Nadezda Urakova, Tanja Strive, Michael FRESE

Research output: Contribution to journalArticle

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Abstract

The extremely pathogenic Rabbit haemorrhagic disease virus (RHDV) and the completely benign Rabbit calicivirus (RCV) are closely related members of the genus Lagovirus (family Caliciviridae). The molecular mechanisms that determine the dramatic difference in virulence are unknown, but indirect evidence suggests that different properties of their RNAdependent RNA polymerases (RdRps) may at least partially be responsible for the contrasting phenotypes. Here we report that the unusual ability of the RHDV RdRp to induce a striking rearrangement of the Golgi network is not specific to RHDV, but a common feature of virulent and benign rabbit caliciviruses alike. Expression of rabbit calicivirus RdRps induced a redistribution of both cis/medial and medial/trans Golgi membrane markers, but not that of an endoplasmic reticulum membrane marker. Inactivating mutations in the conserved GDD motif did not abolish the ability of RHDV RdRp to rearrange the Golgi network, suggesting that polymerase activity and metal co-factors are not required for this function. Finally, we discuss possible implications of RdRp-induced membrane rearrangements on virus replication and host immune responses.
Original languageEnglish
Article numbere0169913
Pages (from-to)1-15
Number of pages15
JournalPLoS One
Volume12
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

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Rabbit Haemorrhagic Disease Virus
RNA-directed RNA polymerase
Rabbit hemorrhagic disease virus
RNA Replicase
Viruses
Rabbits
Membranes
DNA-Directed RNA Polymerases
Lagovirus
Caliciviridae
DNA-directed RNA polymerase
Virus Replication
Endoplasmic Reticulum
Virulence
Metals
Phenotype
Mutation
virus replication
endoplasmic reticulum
virulence

Cite this

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abstract = "The extremely pathogenic Rabbit haemorrhagic disease virus (RHDV) and the completely benign Rabbit calicivirus (RCV) are closely related members of the genus Lagovirus (family Caliciviridae). The molecular mechanisms that determine the dramatic difference in virulence are unknown, but indirect evidence suggests that different properties of their RNAdependent RNA polymerases (RdRps) may at least partially be responsible for the contrasting phenotypes. Here we report that the unusual ability of the RHDV RdRp to induce a striking rearrangement of the Golgi network is not specific to RHDV, but a common feature of virulent and benign rabbit caliciviruses alike. Expression of rabbit calicivirus RdRps induced a redistribution of both cis/medial and medial/trans Golgi membrane markers, but not that of an endoplasmic reticulum membrane marker. Inactivating mutations in the conserved GDD motif did not abolish the ability of RHDV RdRp to rearrange the Golgi network, suggesting that polymerase activity and metal co-factors are not required for this function. Finally, we discuss possible implications of RdRp-induced membrane rearrangements on virus replication and host immune responses.",
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RNA-dependent RNA polymerases of both virulent and benign Rabbit caliciviruses induce striking rearrangement of Golgi membranes. / Urakova, Nadezda; Strive, Tanja; FRESE, Michael.

In: PLoS One, Vol. 12, No. 1, e0169913, 01.01.2017, p. 1-15.

Research output: Contribution to journalArticle

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AB - The extremely pathogenic Rabbit haemorrhagic disease virus (RHDV) and the completely benign Rabbit calicivirus (RCV) are closely related members of the genus Lagovirus (family Caliciviridae). The molecular mechanisms that determine the dramatic difference in virulence are unknown, but indirect evidence suggests that different properties of their RNAdependent RNA polymerases (RdRps) may at least partially be responsible for the contrasting phenotypes. Here we report that the unusual ability of the RHDV RdRp to induce a striking rearrangement of the Golgi network is not specific to RHDV, but a common feature of virulent and benign rabbit caliciviruses alike. Expression of rabbit calicivirus RdRps induced a redistribution of both cis/medial and medial/trans Golgi membrane markers, but not that of an endoplasmic reticulum membrane marker. Inactivating mutations in the conserved GDD motif did not abolish the ability of RHDV RdRp to rearrange the Golgi network, suggesting that polymerase activity and metal co-factors are not required for this function. Finally, we discuss possible implications of RdRp-induced membrane rearrangements on virus replication and host immune responses.

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KW - Virulence/genetics

KW - Amino Acid Motifs

KW - Viral Proteins/chemistry

KW - Animals

KW - Hemorrhagic Disease Virus, Rabbit/pathogenicity

KW - Virus Replication

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