Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells

Anne M. Johnson-Flanagan, Zhong Huiwen, Mohan R. Thiagarajah, Hargurdeep S. Saini

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Brassica napus suspension-cultured cells could be hardened in 6 days at 25°C by the addition of mefluidide or ABA to the culture medium. Cells treated with mefluidide (10 milligrams per liter) or ABA (50 micromolar) attained an LT50 of -17.5°C or -18°C, respectively, while the LT50 for the comparable nonhardened control (sucrose) was -10°C. The increased freezing tolerance of mefluidide-treated cells was paralleled by a 4- to 23-fold increase in ABA, as measured by gas-liquid chromatography using electron capture detection. Application of 1 milligram per liter of fluridone, an inhibitor of abscisic acid biosynthesis, prevented the mefluidide-induced increase in freezing tolerance and the accumulation of ABA. Both these inhibitory effects of fluridone were overridden by 50 micromolar ABA in the culture medium. On the basis of these results, we concluded that increased ABA levels are important for the induction of freezing tolerance in suspension-cultured cells.

Original languageEnglish
Pages (from-to)1044-1048
Number of pages5
JournalPlant Physiology
Volume95
Issue number4
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Fingerprint

Brassica napus
Abscisic Acid
cold tolerance
cultured cells
Freezing
mefluidide
abscisic acid
Cultured Cells
Suspensions
Culture Media
fluridone
Gas Chromatography
Sucrose
culture media
Electrons
Mefluidide
gas chromatography
electrons
biosynthesis
sucrose

Cite this

Johnson-Flanagan, Anne M. ; Huiwen, Zhong ; Thiagarajah, Mohan R. ; Saini, Hargurdeep S. / Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells. In: Plant Physiology. 1991 ; Vol. 95, No. 4. pp. 1044-1048.
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Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells. / Johnson-Flanagan, Anne M.; Huiwen, Zhong; Thiagarajah, Mohan R.; Saini, Hargurdeep S.

In: Plant Physiology, Vol. 95, No. 4, 01.01.1991, p. 1044-1048.

Research output: Contribution to journalArticle

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