Role of envelope N-linked glycosylation in Ross River virus virulence and transmission

Michelle Nelson, Lara Herrero, Jason Jeffery, Marion Hoehn, Penny Rudd, Aroon Supramaniam, Brian Kay, Peter Ryan, Suresh MAHALINGAM

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6 Citations (Scopus)

Abstract

With an expanding geographical range and no specific treatments, human arthritogenic alphaviral disease poses a significant problem worldwide. Previous in vitro work with Ross River virus (RRV) demonstrated that alphaviral N-linked glycosylation contributes to type I IFN (IFN-aß) induction in myeloid dendritic cells. This study further evaluated the role of alphaviral N-linked glycans in vivo, assessing the effect of glycosylation on pathogenesis in a mouse model of RRV-induced disease and on viral infection and dissemination in a common mosquito vector, Aedes vigilax. A viral mutant lacking the E1-141 glycosylation site was attenuated for virus-induced disease, with reduced myositis and higher levels of IFN-¿ induction at peak disease contributing to improved viral clearance, suggesting that glycosylation of the E1 glycoprotein plays a major role in the pathogenesis of RRV. Interestingly, RRV lacking E2-200 glycan had significantly reduced replication in the mosquito vector A. vigilax, whereas loss of either of the E1 or E2-262 glycans had little effect on the competence of the mosquito vector. Overall, these results indicate that glycosylation of the E1 and E2 glycoproteins of RRV provides important determinants of viral virulence and immunopathology in the mammalian host and replication in the mosquito vector.
Original languageEnglish
Article number000412
Pages (from-to)1094-1106
Number of pages13
JournalJournal of General Virology
Volume97
Issue number5
DOIs
Publication statusPublished - 2016

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    Nelson, M., Herrero, L., Jeffery, J., Hoehn, M., Rudd, P., Supramaniam, A., Kay, B., Ryan, P., & MAHALINGAM, S. (2016). Role of envelope N-linked glycosylation in Ross River virus virulence and transmission. Journal of General Virology, 97(5), 1094-1106. [000412]. https://doi.org/10.1099/jgv.0.000412