Rotator cuff tear degeneration and cell apoptosis in smokers versus nonsmokers

Kirsten Lundgreen, Oystein Lian, Alexander Scott, Paulina Nassab, Angie FEARON, Lars Engebretsen

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

PURPOSE: The purpose of this study was to assess the effect of smoking on supraspinatus tendon degeneration, including cellular alterations, proliferation, and apoptosis of tendon cells.

METHODS: Supraspinatus tendon samples of 10 smokers and 15 nonsmokers with full-thickness tears were compared, focusing on the severity of tendon histopathology including apoptosis (programmed cell death), cellularity, and proliferation. Immunohistochemistry was used to assess the density of apoptotic cells and proliferation. The extent of tendon degeneration was classified according to a revised version of the Bonar tendon histopathology score.

RESULTS: The smokers were younger (P = .01). The symptom duration among smokers was longer (P < .05). The supraspinatus tendons from the smokers presented significantly more advanced degenerative changes (Bonar score, 13.5 [interquartile range, 1.4] v 9 [interquartile range, 3]; P < .001). The smokers' tendons showed increased density of apoptotic cells (0.108 [SE, 0.038] v 0.0107 [SE, 0.007]; P = .024) accompanied by reduced tenocyte density (P = .019) and upregulation of proliferative activity (P < .0001).

CONCLUSIONS: Smoking is associated with worsened supraspinatus tendon histopathology and increased apoptosis.

CLINICAL RELEVANCE: Pronounced degenerative changes, reduced tendon cellularity, and increased apoptosis may indicate reduced tendon healing capacity in smokers.

Original languageEnglish
Pages (from-to)936-941
Number of pages6
JournalArthroscopy: The Journal of Arthroscopy and Related Surgery
Volume30
Issue number8
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Tendons
Apoptosis
Rotator Cuff
Cell Proliferation
Rotator Cuff Injuries
Smoking
Tears
Cell Death
Up-Regulation
Cell Count
Immunohistochemistry

Cite this

Lundgreen, Kirsten ; Lian, Oystein ; Scott, Alexander ; Nassab, Paulina ; FEARON, Angie ; Engebretsen, Lars. / Rotator cuff tear degeneration and cell apoptosis in smokers versus nonsmokers. In: Arthroscopy: The Journal of Arthroscopy and Related Surgery. 2014 ; Vol. 30, No. 8. pp. 936-941.
@article{80d57a167d0546489e9a9c9bb93f5e24,
title = "Rotator cuff tear degeneration and cell apoptosis in smokers versus nonsmokers",
abstract = "PURPOSE: The purpose of this study was to assess the effect of smoking on supraspinatus tendon degeneration, including cellular alterations, proliferation, and apoptosis of tendon cells.METHODS: Supraspinatus tendon samples of 10 smokers and 15 nonsmokers with full-thickness tears were compared, focusing on the severity of tendon histopathology including apoptosis (programmed cell death), cellularity, and proliferation. Immunohistochemistry was used to assess the density of apoptotic cells and proliferation. The extent of tendon degeneration was classified according to a revised version of the Bonar tendon histopathology score.RESULTS: The smokers were younger (P = .01). The symptom duration among smokers was longer (P < .05). The supraspinatus tendons from the smokers presented significantly more advanced degenerative changes (Bonar score, 13.5 [interquartile range, 1.4] v 9 [interquartile range, 3]; P < .001). The smokers' tendons showed increased density of apoptotic cells (0.108 [SE, 0.038] v 0.0107 [SE, 0.007]; P = .024) accompanied by reduced tenocyte density (P = .019) and upregulation of proliferative activity (P < .0001).CONCLUSIONS: Smoking is associated with worsened supraspinatus tendon histopathology and increased apoptosis.CLINICAL RELEVANCE: Pronounced degenerative changes, reduced tendon cellularity, and increased apoptosis may indicate reduced tendon healing capacity in smokers.",
keywords = "Apoptosis, Cell Proliferation, Female, Humans, Male, Middle Aged, Rotator Cuff Injuries, Rotator Cuff/pathology, Smoking/adverse effects, Wound Healing",
author = "Kirsten Lundgreen and Oystein Lian and Alexander Scott and Paulina Nassab and Angie FEARON and Lars Engebretsen",
year = "2014",
doi = "10.1016/j.arthro.2014.03.027",
language = "English",
volume = "30",
pages = "936--941",
journal = "Arthroscopy - Journal of Arthroscopic and Related Surgery",
issn = "0749-8063",
publisher = "W.B. Saunders Ltd",
number = "8",

}

Rotator cuff tear degeneration and cell apoptosis in smokers versus nonsmokers. / Lundgreen, Kirsten; Lian, Oystein; Scott, Alexander; Nassab, Paulina; FEARON, Angie; Engebretsen, Lars.

In: Arthroscopy: The Journal of Arthroscopy and Related Surgery, Vol. 30, No. 8, 2014, p. 936-941.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rotator cuff tear degeneration and cell apoptosis in smokers versus nonsmokers

AU - Lundgreen, Kirsten

AU - Lian, Oystein

AU - Scott, Alexander

AU - Nassab, Paulina

AU - FEARON, Angie

AU - Engebretsen, Lars

PY - 2014

Y1 - 2014

N2 - PURPOSE: The purpose of this study was to assess the effect of smoking on supraspinatus tendon degeneration, including cellular alterations, proliferation, and apoptosis of tendon cells.METHODS: Supraspinatus tendon samples of 10 smokers and 15 nonsmokers with full-thickness tears were compared, focusing on the severity of tendon histopathology including apoptosis (programmed cell death), cellularity, and proliferation. Immunohistochemistry was used to assess the density of apoptotic cells and proliferation. The extent of tendon degeneration was classified according to a revised version of the Bonar tendon histopathology score.RESULTS: The smokers were younger (P = .01). The symptom duration among smokers was longer (P < .05). The supraspinatus tendons from the smokers presented significantly more advanced degenerative changes (Bonar score, 13.5 [interquartile range, 1.4] v 9 [interquartile range, 3]; P < .001). The smokers' tendons showed increased density of apoptotic cells (0.108 [SE, 0.038] v 0.0107 [SE, 0.007]; P = .024) accompanied by reduced tenocyte density (P = .019) and upregulation of proliferative activity (P < .0001).CONCLUSIONS: Smoking is associated with worsened supraspinatus tendon histopathology and increased apoptosis.CLINICAL RELEVANCE: Pronounced degenerative changes, reduced tendon cellularity, and increased apoptosis may indicate reduced tendon healing capacity in smokers.

AB - PURPOSE: The purpose of this study was to assess the effect of smoking on supraspinatus tendon degeneration, including cellular alterations, proliferation, and apoptosis of tendon cells.METHODS: Supraspinatus tendon samples of 10 smokers and 15 nonsmokers with full-thickness tears were compared, focusing on the severity of tendon histopathology including apoptosis (programmed cell death), cellularity, and proliferation. Immunohistochemistry was used to assess the density of apoptotic cells and proliferation. The extent of tendon degeneration was classified according to a revised version of the Bonar tendon histopathology score.RESULTS: The smokers were younger (P = .01). The symptom duration among smokers was longer (P < .05). The supraspinatus tendons from the smokers presented significantly more advanced degenerative changes (Bonar score, 13.5 [interquartile range, 1.4] v 9 [interquartile range, 3]; P < .001). The smokers' tendons showed increased density of apoptotic cells (0.108 [SE, 0.038] v 0.0107 [SE, 0.007]; P = .024) accompanied by reduced tenocyte density (P = .019) and upregulation of proliferative activity (P < .0001).CONCLUSIONS: Smoking is associated with worsened supraspinatus tendon histopathology and increased apoptosis.CLINICAL RELEVANCE: Pronounced degenerative changes, reduced tendon cellularity, and increased apoptosis may indicate reduced tendon healing capacity in smokers.

KW - Apoptosis

KW - Cell Proliferation

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Rotator Cuff Injuries

KW - Rotator Cuff/pathology

KW - Smoking/adverse effects

KW - Wound Healing

UR - http://www.scopus.com/inward/record.url?scp=84905578055&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/rotator-cuff-tear-degeneration-cell-apoptosis-smokers-versus-nonsmokers

U2 - 10.1016/j.arthro.2014.03.027

DO - 10.1016/j.arthro.2014.03.027

M3 - Article

VL - 30

SP - 936

EP - 941

JO - Arthroscopy - Journal of Arthroscopic and Related Surgery

JF - Arthroscopy - Journal of Arthroscopic and Related Surgery

SN - 0749-8063

IS - 8

ER -