TY - JOUR
T1 - Satellitome analysis illuminates the evolution of ZW sex chromosomes of Triportheidae fishes (Teleostei Characiformes)
AU - Kretschmer, Rafael
AU - Goes, Caio Augusto Gomes
AU - Bertollo, Luiz Antônio Carlos
AU - Ezaz, Tariq
AU - Porto-Foresti, Fábio
AU - Toma, Gustavo Akira
AU - Utsunomia, Ricardo
AU - de Bello Cioffi, Marcelo
N1 - Funding Information:
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (Proc. no 302449/2018–3 for MBC and 405334/2017–4 for RU), Fundação de Amparo à Pesquisa do Estado de São Paulo- FAPESP (RK, Proc. No. 2020/11778–2; MBC, Proc. No. 2020/11669–2; GAT, Proc. No. 2018/14677–6), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro- FAPERJ (Proc. Nos. 201.289/2021 and 211.475/2019), and TE was partially supported by an Australian Research Council Discovery Grant DP200101406 led by Erik Wapstra, Tariq Ezaz, Cristopher Burridge, and Oleg Simakov.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Funding Information:
We would like to thank all the staff of the ?Laborat?rio de Citogen?tica de Peixes? from the Universidade Federal de S?o Carlos for their technical support.
Funding Information:
This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (Proc. no 302449/2018–3 for MBC and 405334/2017–4 for RU), Fundação de Amparo à Pesquisa do Estado de São Paulo- FAPESP (RK, Proc. No. 2020/11778–2; MBC, Proc. No. 2020/11669–2; GAT, Proc. No. 2018/14677–6), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro- FAPERJ (Proc. Nos. 201.289/2021 and 211.475/2019), and TE was partially supported by an Australian Research Council Discovery Grant DP200101406 led by Erik Wapstra, Tariq Ezaz, Cristopher Burridge, and Oleg Simakov.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2022/6
Y1 - 2022/6
N2 - Satellites are an abundant source of repetitive DNAs that play an essential role in the chromosomal organization and are tightly linked with the evolution of sex chromosomes. Among fishes, Triportheidae stands out as the only family where almost all species have a homeologous ZZ/ZW sex chromosomes system. While the Z chromosome is typically conserved, the W is always smaller, with variations in size and morphology between species. Here, we report an analysis of the satellitome of Triportheus auritus (TauSat) by integrating genomic and chromosomal data, with a special focus on the highly abundant and female-biased satDNAs. In addition, we investigated the evolutionary trajectories of the ZW sex chromosomes in the Triportheidae family by mapping satDNAs in selected representative species of this family. The satellitome of T. auritus comprised 53 satDNA families of which 24 were also hybridized by FISH. Most satDNAs differed significantly between sexes, with 19 out of 24 being enriched on the W chromosome of T. auritus. The number of satDNAs hybridized into the W chromosomes of T. signatus and T. albus decreased to six and four, respectively, in accordance with the size of their W chromosomes. No TauSat probes produced FISH signals on the chromosomes of Agoniates halecinus. Despite its apparent conservation, our results indicate that each species differs in the satDNA accumulation on the Z chromosome. Minimum spanning trees (MSTs), generated for three satDNA families with different patterns of FISH mapping data, revealed different homogenization rates between the Z and W chromosomes. These results were linked to different levels of recombination between them. The most abundant satDNA family (TauSat01) was exclusively hybridized in the centromeres of all 52 chromosomes of T. auritus, and its putative role in the centromere evolution was also highlighted. Our results identified a high differentiation of both ZW chromosomes regarding satellites composition, highlighting their dynamic role in the sex chromosomes evolution.
AB - Satellites are an abundant source of repetitive DNAs that play an essential role in the chromosomal organization and are tightly linked with the evolution of sex chromosomes. Among fishes, Triportheidae stands out as the only family where almost all species have a homeologous ZZ/ZW sex chromosomes system. While the Z chromosome is typically conserved, the W is always smaller, with variations in size and morphology between species. Here, we report an analysis of the satellitome of Triportheus auritus (TauSat) by integrating genomic and chromosomal data, with a special focus on the highly abundant and female-biased satDNAs. In addition, we investigated the evolutionary trajectories of the ZW sex chromosomes in the Triportheidae family by mapping satDNAs in selected representative species of this family. The satellitome of T. auritus comprised 53 satDNA families of which 24 were also hybridized by FISH. Most satDNAs differed significantly between sexes, with 19 out of 24 being enriched on the W chromosome of T. auritus. The number of satDNAs hybridized into the W chromosomes of T. signatus and T. albus decreased to six and four, respectively, in accordance with the size of their W chromosomes. No TauSat probes produced FISH signals on the chromosomes of Agoniates halecinus. Despite its apparent conservation, our results indicate that each species differs in the satDNA accumulation on the Z chromosome. Minimum spanning trees (MSTs), generated for three satDNA families with different patterns of FISH mapping data, revealed different homogenization rates between the Z and W chromosomes. These results were linked to different levels of recombination between them. The most abundant satDNA family (TauSat01) was exclusively hybridized in the centromeres of all 52 chromosomes of T. auritus, and its putative role in the centromere evolution was also highlighted. Our results identified a high differentiation of both ZW chromosomes regarding satellites composition, highlighting their dynamic role in the sex chromosomes evolution.
KW - FISH
KW - Fishes
KW - High-throughput sequencing
KW - satDNA evolution
KW - Satellitome
KW - Sex chromosome evolution
UR - http://www.scopus.com/inward/record.url?scp=85123948616&partnerID=8YFLogxK
U2 - 10.1007/s00412-022-00768-1
DO - 10.1007/s00412-022-00768-1
M3 - Article
C2 - 35099570
AN - SCOPUS:85123948616
SN - 0009-5915
VL - 131
SP - 29
EP - 45
JO - Chromosoma
JF - Chromosoma
IS - 1-2
ER -