Scrambled and fried

Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress

A. P. Sobinoff, E. L. Beckett, A. G. Jarnicki, J. M. Sutherland, A. McCluskey, P. M. Hansbro, E. A. McLaughlin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cigarette smoke is a reproductive hazard associated with pre-mature reproductive senescence and reduced clinical pregnancy rates in female smokers. Despite an increased awareness of the adverse effects of cigarette smoke exposure on systemic health, many women remain unaware of the adverse effects of cigarette smoke on female fertility. This issue is compounded by our limited understanding of the molecular mechanisms behind cigarette smoke induced infertility. In this study we used a direct nasal exposure mouse model of cigarette smoke-induced chronic obstructive pulmonary disease to characterise mechanisms of cigarette-smoke induced ovotoxicity. Cigarette smoke exposure caused increased levels of primordial follicle depletion, antral follicle oocyte apoptosis and oxidative stress in exposed ovaries, resulting in fewer follicles available for ovulation. Evidence of oxidative stress also persisted in ovulated oocytes which escaped destruction, with increased levels of mitochondrial ROS and lipid peroxidation resulting in reduced fertilisation potential. Microarray analysis of ovarian tissue correlated these insults with a complex mechanism of ovotoxicity involving genes associated with detoxification, inflammation, follicular activation, immune cell mediated apoptosis and membrane organisation. In particular, the phase I detoxifying enzyme cyp2e1 was found to be significantly up-regulated in developing oocytes; an enzyme known to cause molecular bioactivation resulting in oxidative stress. Our results provide a preliminary model of cigarette smoke induced sub-fertility through cyp2e1 bioactivation and oxidative stress, resulting in developing follicle depletion and oocyte dysfunction.

Original languageEnglish
Pages (from-to)156-167
Number of pages12
JournalToxicology and Applied Pharmacology
Volume271
Issue number2
DOIs
Publication statusPublished - 1 Sep 2013
Externally publishedYes

Fingerprint

Oxidative stress
Smoke
Tobacco Products
Oocytes
Oxidative Stress
Infertility
Tissue Array Analysis
Apoptosis
Pulmonary diseases
Detoxification
Antral
Women's Health
Enzymes
Pregnancy Rate
Microarrays
Ovulation
Nose
Fertilization
Chronic Obstructive Pulmonary Disease
Lipid Peroxidation

Cite this

Sobinoff, A. P., Beckett, E. L., Jarnicki, A. G., Sutherland, J. M., McCluskey, A., Hansbro, P. M., & McLaughlin, E. A. (2013). Scrambled and fried: Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress. Toxicology and Applied Pharmacology, 271(2), 156-167. https://doi.org/10.1016/j.taap.2013.05.009
Sobinoff, A. P. ; Beckett, E. L. ; Jarnicki, A. G. ; Sutherland, J. M. ; McCluskey, A. ; Hansbro, P. M. ; McLaughlin, E. A. / Scrambled and fried : Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress. In: Toxicology and Applied Pharmacology. 2013 ; Vol. 271, No. 2. pp. 156-167.
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Sobinoff, AP, Beckett, EL, Jarnicki, AG, Sutherland, JM, McCluskey, A, Hansbro, PM & McLaughlin, EA 2013, 'Scrambled and fried: Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress', Toxicology and Applied Pharmacology, vol. 271, no. 2, pp. 156-167. https://doi.org/10.1016/j.taap.2013.05.009

Scrambled and fried : Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress. / Sobinoff, A. P.; Beckett, E. L.; Jarnicki, A. G.; Sutherland, J. M.; McCluskey, A.; Hansbro, P. M.; McLaughlin, E. A.

In: Toxicology and Applied Pharmacology, Vol. 271, No. 2, 01.09.2013, p. 156-167.

Research output: Contribution to journalArticle

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T2 - Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress

AU - Sobinoff, A. P.

AU - Beckett, E. L.

AU - Jarnicki, A. G.

AU - Sutherland, J. M.

AU - McCluskey, A.

AU - Hansbro, P. M.

AU - McLaughlin, E. A.

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N2 - Cigarette smoke is a reproductive hazard associated with pre-mature reproductive senescence and reduced clinical pregnancy rates in female smokers. Despite an increased awareness of the adverse effects of cigarette smoke exposure on systemic health, many women remain unaware of the adverse effects of cigarette smoke on female fertility. This issue is compounded by our limited understanding of the molecular mechanisms behind cigarette smoke induced infertility. In this study we used a direct nasal exposure mouse model of cigarette smoke-induced chronic obstructive pulmonary disease to characterise mechanisms of cigarette-smoke induced ovotoxicity. Cigarette smoke exposure caused increased levels of primordial follicle depletion, antral follicle oocyte apoptosis and oxidative stress in exposed ovaries, resulting in fewer follicles available for ovulation. Evidence of oxidative stress also persisted in ovulated oocytes which escaped destruction, with increased levels of mitochondrial ROS and lipid peroxidation resulting in reduced fertilisation potential. Microarray analysis of ovarian tissue correlated these insults with a complex mechanism of ovotoxicity involving genes associated with detoxification, inflammation, follicular activation, immune cell mediated apoptosis and membrane organisation. In particular, the phase I detoxifying enzyme cyp2e1 was found to be significantly up-regulated in developing oocytes; an enzyme known to cause molecular bioactivation resulting in oxidative stress. Our results provide a preliminary model of cigarette smoke induced sub-fertility through cyp2e1 bioactivation and oxidative stress, resulting in developing follicle depletion and oocyte dysfunction.

AB - Cigarette smoke is a reproductive hazard associated with pre-mature reproductive senescence and reduced clinical pregnancy rates in female smokers. Despite an increased awareness of the adverse effects of cigarette smoke exposure on systemic health, many women remain unaware of the adverse effects of cigarette smoke on female fertility. This issue is compounded by our limited understanding of the molecular mechanisms behind cigarette smoke induced infertility. In this study we used a direct nasal exposure mouse model of cigarette smoke-induced chronic obstructive pulmonary disease to characterise mechanisms of cigarette-smoke induced ovotoxicity. Cigarette smoke exposure caused increased levels of primordial follicle depletion, antral follicle oocyte apoptosis and oxidative stress in exposed ovaries, resulting in fewer follicles available for ovulation. Evidence of oxidative stress also persisted in ovulated oocytes which escaped destruction, with increased levels of mitochondrial ROS and lipid peroxidation resulting in reduced fertilisation potential. Microarray analysis of ovarian tissue correlated these insults with a complex mechanism of ovotoxicity involving genes associated with detoxification, inflammation, follicular activation, immune cell mediated apoptosis and membrane organisation. In particular, the phase I detoxifying enzyme cyp2e1 was found to be significantly up-regulated in developing oocytes; an enzyme known to cause molecular bioactivation resulting in oxidative stress. Our results provide a preliminary model of cigarette smoke induced sub-fertility through cyp2e1 bioactivation and oxidative stress, resulting in developing follicle depletion and oocyte dysfunction.

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KW - Cigarette

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KW - Oxidative stress

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