Selection on MHC class II supertypes in the New Zealand endemic Hochstetter's frog Phylogenetics and phylogeography

Mette Lillie, Catherine E. Grueber, Jolene Sutton, Robyn Howitt, Phillip Bishop, Dianne GLEESON, Katherine Belov

    Research output: Contribution to journalArticle

    16 Citations (Scopus)
    2 Downloads (Pure)

    Abstract

    Background: The New Zealand native frogs, family Leiopelmatidae, are among the most archaic in the world. Leiopelma hochstetteri (Hochstetter's frog) is a small, semi-aquatic frog with numerous, fragmented populations scattered across New Zealand's North Island. We characterized a major histocompatibility complex (MHC) class II B gene (DAB) in L. hochstetteri from a spleen transcriptome, and then compared its diversity to neutral microsatellite markers to assess the adaptive genetic diversity of five populations ("evolutionarily significant units", ESUs). Results: L. hochstetteri possessed very high MHC diversity, with 74 DAB alleles characterized. Extremely high differentiation was observed at the DAB locus, with only two alleles shared between populations, a pattern that was not reflected in the microsatellites. Clustering analysis on putative peptide binding residues of the DAB alleles indicated four functional supertypes, all of which were represented in 4 of 5 populations, albeit at different frequencies. Otawa was an exception to these observations, with only two DAB alleles present. Conclusions: This study of MHC diversity highlights extreme population differentiation at this functional locus. Supertype differentiation was high among populations, suggesting spatial and/or temporal variation in selection pressures. Low DAB diversity in Otawa may limit this population's adaptive potential to future pathogenic challenges.
    Original languageEnglish
    Pages (from-to)1-11
    Number of pages11
    JournalBMC Evolutionary Biology
    Volume15
    Issue number1
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    major histocompatibility complex
    phylogeography
    frog
    frogs
    allele
    phylogenetics
    phylogeny
    alleles
    peptide
    microsatellite repeats
    temporal variation
    loci
    gene
    transcriptome
    spleen
    peptides
    genetic variation

    Cite this

    Lillie, Mette ; Grueber, Catherine E. ; Sutton, Jolene ; Howitt, Robyn ; Bishop, Phillip ; GLEESON, Dianne ; Belov, Katherine. / Selection on MHC class II supertypes in the New Zealand endemic Hochstetter's frog Phylogenetics and phylogeography. In: BMC Evolutionary Biology. 2015 ; Vol. 15, No. 1. pp. 1-11.
    @article{8569e07d4bb6472293eb228e43ea453f,
    title = "Selection on MHC class II supertypes in the New Zealand endemic Hochstetter's frog Phylogenetics and phylogeography",
    abstract = "Background: The New Zealand native frogs, family Leiopelmatidae, are among the most archaic in the world. Leiopelma hochstetteri (Hochstetter's frog) is a small, semi-aquatic frog with numerous, fragmented populations scattered across New Zealand's North Island. We characterized a major histocompatibility complex (MHC) class II B gene (DAB) in L. hochstetteri from a spleen transcriptome, and then compared its diversity to neutral microsatellite markers to assess the adaptive genetic diversity of five populations ({"}evolutionarily significant units{"}, ESUs). Results: L. hochstetteri possessed very high MHC diversity, with 74 DAB alleles characterized. Extremely high differentiation was observed at the DAB locus, with only two alleles shared between populations, a pattern that was not reflected in the microsatellites. Clustering analysis on putative peptide binding residues of the DAB alleles indicated four functional supertypes, all of which were represented in 4 of 5 populations, albeit at different frequencies. Otawa was an exception to these observations, with only two DAB alleles present. Conclusions: This study of MHC diversity highlights extreme population differentiation at this functional locus. Supertype differentiation was high among populations, suggesting spatial and/or temporal variation in selection pressures. Low DAB diversity in Otawa may limit this population's adaptive potential to future pathogenic challenges.",
    author = "Mette Lillie and Grueber, {Catherine E.} and Jolene Sutton and Robyn Howitt and Phillip Bishop and Dianne GLEESON and Katherine Belov",
    year = "2015",
    doi = "10.1186/s12862-015-0342-0",
    language = "English",
    volume = "15",
    pages = "1--11",
    journal = "BMC Evolutionary Biology",
    issn = "1471-2148",
    publisher = "BioMed Central",
    number = "1",

    }

    Selection on MHC class II supertypes in the New Zealand endemic Hochstetter's frog Phylogenetics and phylogeography. / Lillie, Mette; Grueber, Catherine E.; Sutton, Jolene; Howitt, Robyn; Bishop, Phillip; GLEESON, Dianne; Belov, Katherine.

    In: BMC Evolutionary Biology, Vol. 15, No. 1, 2015, p. 1-11.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Selection on MHC class II supertypes in the New Zealand endemic Hochstetter's frog Phylogenetics and phylogeography

    AU - Lillie, Mette

    AU - Grueber, Catherine E.

    AU - Sutton, Jolene

    AU - Howitt, Robyn

    AU - Bishop, Phillip

    AU - GLEESON, Dianne

    AU - Belov, Katherine

    PY - 2015

    Y1 - 2015

    N2 - Background: The New Zealand native frogs, family Leiopelmatidae, are among the most archaic in the world. Leiopelma hochstetteri (Hochstetter's frog) is a small, semi-aquatic frog with numerous, fragmented populations scattered across New Zealand's North Island. We characterized a major histocompatibility complex (MHC) class II B gene (DAB) in L. hochstetteri from a spleen transcriptome, and then compared its diversity to neutral microsatellite markers to assess the adaptive genetic diversity of five populations ("evolutionarily significant units", ESUs). Results: L. hochstetteri possessed very high MHC diversity, with 74 DAB alleles characterized. Extremely high differentiation was observed at the DAB locus, with only two alleles shared between populations, a pattern that was not reflected in the microsatellites. Clustering analysis on putative peptide binding residues of the DAB alleles indicated four functional supertypes, all of which were represented in 4 of 5 populations, albeit at different frequencies. Otawa was an exception to these observations, with only two DAB alleles present. Conclusions: This study of MHC diversity highlights extreme population differentiation at this functional locus. Supertype differentiation was high among populations, suggesting spatial and/or temporal variation in selection pressures. Low DAB diversity in Otawa may limit this population's adaptive potential to future pathogenic challenges.

    AB - Background: The New Zealand native frogs, family Leiopelmatidae, are among the most archaic in the world. Leiopelma hochstetteri (Hochstetter's frog) is a small, semi-aquatic frog with numerous, fragmented populations scattered across New Zealand's North Island. We characterized a major histocompatibility complex (MHC) class II B gene (DAB) in L. hochstetteri from a spleen transcriptome, and then compared its diversity to neutral microsatellite markers to assess the adaptive genetic diversity of five populations ("evolutionarily significant units", ESUs). Results: L. hochstetteri possessed very high MHC diversity, with 74 DAB alleles characterized. Extremely high differentiation was observed at the DAB locus, with only two alleles shared between populations, a pattern that was not reflected in the microsatellites. Clustering analysis on putative peptide binding residues of the DAB alleles indicated four functional supertypes, all of which were represented in 4 of 5 populations, albeit at different frequencies. Otawa was an exception to these observations, with only two DAB alleles present. Conclusions: This study of MHC diversity highlights extreme population differentiation at this functional locus. Supertype differentiation was high among populations, suggesting spatial and/or temporal variation in selection pressures. Low DAB diversity in Otawa may limit this population's adaptive potential to future pathogenic challenges.

    U2 - 10.1186/s12862-015-0342-0

    DO - 10.1186/s12862-015-0342-0

    M3 - Article

    VL - 15

    SP - 1

    EP - 11

    JO - BMC Evolutionary Biology

    JF - BMC Evolutionary Biology

    SN - 1471-2148

    IS - 1

    ER -