Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers

Bill MAHER, Rajani Jagtap, Margaret Roper, Cathryn O'Sullivan

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    Abstract

    This study investigated whether selenium species in wheat grains could be altered by exposure to different combinations of nitrogen (N) and sulphur (S) fertilisers in an agronomic biofortification experiment. Four Australian wheat cultivars (Mace, Janz, Emu Rock and Magenta) were grown in a glasshouse experiment and exposed to 3 mg Se kg−1 soil as selenate (SeVI). Plants were also exposed to 60 mg N kg−1 soil as urea and 20 mg S kg−1 soil as gypsum in a factorial design (N + S + Se; N + Se; S + Se; Se only). Plants were grown to maturity with grain analysed for total Se concentrations via ICP-MS and Se species determined via HPLC-ICP-MS. Grain Se concentrations ranged from 22 to 70 µg Se g−1 grain (dry mass). Selenomethionine (SeMet), Se-methylselenocystine (MeSeCys), selenohomolanthionine (SeHLan), plus a large concentration of uncharacterised Se species were found in the extracts from grains. SeMet was the major Se species identified accounting for between 9 and 24 µg Se g−1 grain. Exposure to different N and S fertiliser combinations altered the SeMet content of Mace, Janz and Emu Rock grain, but not that of Magenta. MeSeCys and SeHLan were found in far lower concentrations (<4 µg Se g−1 grain). A large component of the total grain Se was uncharacterisable (>30 % of total grain Se) in all samples. When N fertiliser was applied (with or without S), the proportion of uncharacterisable Se increased between 60 and 70 % of the total grain Se. The data presented here indicate that it is possible to alter the content of individual Se species in wheat grains via biofortification combined with manipulation of N and S fertiliser regimes. This has potential significance in alleviating or combating both Se deficiency and Se toxicity effects in humans
    Original languageEnglish
    Pages (from-to)1-12
    Number of pages12
    JournalEnvironmental Geochemistry and Health
    VolumeOnline
    DOIs
    Publication statusPublished - 2017

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    Fertilizers
    Selenium
    Selenomethionine
    selenium
    Sulfur
    Triticum
    omega-Chloroacetophenone
    Nitrogen
    Dromaiidae
    wheat
    sulfur
    fertilizer
    Soil
    nitrogen
    Selenic Acid
    Calcium Sulfate
    selenate
    soil
    Urea
    Soils

    Cite this

    MAHER, Bill ; Jagtap, Rajani ; Roper, Margaret ; O'Sullivan, Cathryn. / Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers. In: Environmental Geochemistry and Health. 2017 ; Vol. Online. pp. 1-12.
    @article{1857b05477874a929768238fa6b5c4df,
    title = "Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers",
    abstract = "This study investigated whether selenium species in wheat grains could be altered by exposure to different combinations of nitrogen (N) and sulphur (S) fertilisers in an agronomic biofortification experiment. Four Australian wheat cultivars (Mace, Janz, Emu Rock and Magenta) were grown in a glasshouse experiment and exposed to 3 mg Se kg−1 soil as selenate (SeVI). Plants were also exposed to 60 mg N kg−1 soil as urea and 20 mg S kg−1 soil as gypsum in a factorial design (N + S + Se; N + Se; S + Se; Se only). Plants were grown to maturity with grain analysed for total Se concentrations via ICP-MS and Se species determined via HPLC-ICP-MS. Grain Se concentrations ranged from 22 to 70 µg Se g−1 grain (dry mass). Selenomethionine (SeMet), Se-methylselenocystine (MeSeCys), selenohomolanthionine (SeHLan), plus a large concentration of uncharacterised Se species were found in the extracts from grains. SeMet was the major Se species identified accounting for between 9 and 24 µg Se g−1 grain. Exposure to different N and S fertiliser combinations altered the SeMet content of Mace, Janz and Emu Rock grain, but not that of Magenta. MeSeCys and SeHLan were found in far lower concentrations (<4 µg Se g−1 grain). A large component of the total grain Se was uncharacterisable (>30 {\%} of total grain Se) in all samples. When N fertiliser was applied (with or without S), the proportion of uncharacterisable Se increased between 60 and 70 {\%} of the total grain Se. The data presented here indicate that it is possible to alter the content of individual Se species in wheat grains via biofortification combined with manipulation of N and S fertiliser regimes. This has potential significance in alleviating or combating both Se deficiency and Se toxicity effects in humans",
    author = "Bill MAHER and Rajani Jagtap and Margaret Roper and Cathryn O'Sullivan",
    year = "2017",
    doi = "10.1007/s10653-016-9857-6",
    language = "English",
    volume = "Online",
    pages = "1--12",
    journal = "Environmental Geochemistry and Health",
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    Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers. / MAHER, Bill; Jagtap, Rajani; Roper, Margaret; O'Sullivan, Cathryn.

    In: Environmental Geochemistry and Health, Vol. Online, 2017, p. 1-12.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers

    AU - MAHER, Bill

    AU - Jagtap, Rajani

    AU - Roper, Margaret

    AU - O'Sullivan, Cathryn

    PY - 2017

    Y1 - 2017

    N2 - This study investigated whether selenium species in wheat grains could be altered by exposure to different combinations of nitrogen (N) and sulphur (S) fertilisers in an agronomic biofortification experiment. Four Australian wheat cultivars (Mace, Janz, Emu Rock and Magenta) were grown in a glasshouse experiment and exposed to 3 mg Se kg−1 soil as selenate (SeVI). Plants were also exposed to 60 mg N kg−1 soil as urea and 20 mg S kg−1 soil as gypsum in a factorial design (N + S + Se; N + Se; S + Se; Se only). Plants were grown to maturity with grain analysed for total Se concentrations via ICP-MS and Se species determined via HPLC-ICP-MS. Grain Se concentrations ranged from 22 to 70 µg Se g−1 grain (dry mass). Selenomethionine (SeMet), Se-methylselenocystine (MeSeCys), selenohomolanthionine (SeHLan), plus a large concentration of uncharacterised Se species were found in the extracts from grains. SeMet was the major Se species identified accounting for between 9 and 24 µg Se g−1 grain. Exposure to different N and S fertiliser combinations altered the SeMet content of Mace, Janz and Emu Rock grain, but not that of Magenta. MeSeCys and SeHLan were found in far lower concentrations (<4 µg Se g−1 grain). A large component of the total grain Se was uncharacterisable (>30 % of total grain Se) in all samples. When N fertiliser was applied (with or without S), the proportion of uncharacterisable Se increased between 60 and 70 % of the total grain Se. The data presented here indicate that it is possible to alter the content of individual Se species in wheat grains via biofortification combined with manipulation of N and S fertiliser regimes. This has potential significance in alleviating or combating both Se deficiency and Se toxicity effects in humans

    AB - This study investigated whether selenium species in wheat grains could be altered by exposure to different combinations of nitrogen (N) and sulphur (S) fertilisers in an agronomic biofortification experiment. Four Australian wheat cultivars (Mace, Janz, Emu Rock and Magenta) were grown in a glasshouse experiment and exposed to 3 mg Se kg−1 soil as selenate (SeVI). Plants were also exposed to 60 mg N kg−1 soil as urea and 20 mg S kg−1 soil as gypsum in a factorial design (N + S + Se; N + Se; S + Se; Se only). Plants were grown to maturity with grain analysed for total Se concentrations via ICP-MS and Se species determined via HPLC-ICP-MS. Grain Se concentrations ranged from 22 to 70 µg Se g−1 grain (dry mass). Selenomethionine (SeMet), Se-methylselenocystine (MeSeCys), selenohomolanthionine (SeHLan), plus a large concentration of uncharacterised Se species were found in the extracts from grains. SeMet was the major Se species identified accounting for between 9 and 24 µg Se g−1 grain. Exposure to different N and S fertiliser combinations altered the SeMet content of Mace, Janz and Emu Rock grain, but not that of Magenta. MeSeCys and SeHLan were found in far lower concentrations (<4 µg Se g−1 grain). A large component of the total grain Se was uncharacterisable (>30 % of total grain Se) in all samples. When N fertiliser was applied (with or without S), the proportion of uncharacterisable Se increased between 60 and 70 % of the total grain Se. The data presented here indicate that it is possible to alter the content of individual Se species in wheat grains via biofortification combined with manipulation of N and S fertiliser regimes. This has potential significance in alleviating or combating both Se deficiency and Se toxicity effects in humans

    U2 - 10.1007/s10653-016-9857-6

    DO - 10.1007/s10653-016-9857-6

    M3 - Article

    VL - Online

    SP - 1

    EP - 12

    JO - Environmental Geochemistry and Health

    JF - Environmental Geochemistry and Health

    SN - 0269-4042

    ER -