Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium

F Aborobe, A Raab, Simon FOSTER, E. Lombi, Bill MAHER, E Krupp, J Feldmann

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Three month old Thunbergia alata were exposed for 13 days to 10 µM selenite to determine the biotransformation of selenite in their roots. Selenium in formic acid extracts (80 ± 3%) was present as selenopeptides with Se-S bonds and selenium-PC complexes (selenocysteinyl-2-3-dihydroxypropionyl-glutathione, seleno-phytochelatin2, seleno-di-glutathione). An analytical method using HPLC-ICPMS to detect and quantify elemental selenium in roots of T. alata plants using sodium sulfite to quantitatively transform elemental selenium to selenosulfate was also developed. Elemental selenium was determined as 18 ± 4% of the total selenium in the roots which was equivalent to the selenium not extracted using formic acid extraction. The results are in an agreement with the XAS measurements of the exposed roots which showed no occurrence of selenite or selenate but a mixture of selenocysteine and elemental selenium.
    Original languageEnglish
    Pages (from-to)1056-1066
    Number of pages11
    JournalMetallomics
    Volume7
    Issue number7
    DOIs
    Publication statusPublished - 2015

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    Acanthaceae
    Selenious Acid
    Selenium
    formic acid
    Formic acid
    Selenic Acid
    Selenocysteine
    Biotransformation
    Glutathione
    High Pressure Liquid Chromatography
    Sodium

    Cite this

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    title = "Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium",
    abstract = "Three month old Thunbergia alata were exposed for 13 days to 10 µM selenite to determine the biotransformation of selenite in their roots. Selenium in formic acid extracts (80 ± 3{\%}) was present as selenopeptides with Se-S bonds and selenium-PC complexes (selenocysteinyl-2-3-dihydroxypropionyl-glutathione, seleno-phytochelatin2, seleno-di-glutathione). An analytical method using HPLC-ICPMS to detect and quantify elemental selenium in roots of T. alata plants using sodium sulfite to quantitatively transform elemental selenium to selenosulfate was also developed. Elemental selenium was determined as 18 ± 4{\%} of the total selenium in the roots which was equivalent to the selenium not extracted using formic acid extraction. The results are in an agreement with the XAS measurements of the exposed roots which showed no occurrence of selenite or selenate but a mixture of selenocysteine and elemental selenium.",
    author = "F Aborobe and A Raab and Simon FOSTER and E. Lombi and Bill MAHER and E Krupp and J Feldmann",
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    Selenopeptides and elemental selenium in Thunbergia alata after exposure to selenite: quantification method for elemental selenium. / Aborobe, F; Raab, A; FOSTER, Simon; Lombi, E.; MAHER, Bill; Krupp, E; Feldmann, J.

    In: Metallomics, Vol. 7, No. 7, 2015, p. 1056-1066.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Aborobe, F

    AU - Raab, A

    AU - FOSTER, Simon

    AU - Lombi, E.

    AU - MAHER, Bill

    AU - Krupp, E

    AU - Feldmann, J

    PY - 2015

    Y1 - 2015

    N2 - Three month old Thunbergia alata were exposed for 13 days to 10 µM selenite to determine the biotransformation of selenite in their roots. Selenium in formic acid extracts (80 ± 3%) was present as selenopeptides with Se-S bonds and selenium-PC complexes (selenocysteinyl-2-3-dihydroxypropionyl-glutathione, seleno-phytochelatin2, seleno-di-glutathione). An analytical method using HPLC-ICPMS to detect and quantify elemental selenium in roots of T. alata plants using sodium sulfite to quantitatively transform elemental selenium to selenosulfate was also developed. Elemental selenium was determined as 18 ± 4% of the total selenium in the roots which was equivalent to the selenium not extracted using formic acid extraction. The results are in an agreement with the XAS measurements of the exposed roots which showed no occurrence of selenite or selenate but a mixture of selenocysteine and elemental selenium.

    AB - Three month old Thunbergia alata were exposed for 13 days to 10 µM selenite to determine the biotransformation of selenite in their roots. Selenium in formic acid extracts (80 ± 3%) was present as selenopeptides with Se-S bonds and selenium-PC complexes (selenocysteinyl-2-3-dihydroxypropionyl-glutathione, seleno-phytochelatin2, seleno-di-glutathione). An analytical method using HPLC-ICPMS to detect and quantify elemental selenium in roots of T. alata plants using sodium sulfite to quantitatively transform elemental selenium to selenosulfate was also developed. Elemental selenium was determined as 18 ± 4% of the total selenium in the roots which was equivalent to the selenium not extracted using formic acid extraction. The results are in an agreement with the XAS measurements of the exposed roots which showed no occurrence of selenite or selenate but a mixture of selenocysteine and elemental selenium.

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