Short-term reliability of inflammatory mediators and response to exercise in the heat

Joshua H. Guy, Andrew M. Edwards, Catherine M. Miller, Glen B. Deakin, David B. Pyne

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Prospective application of serum cytokines, lipopolysaccharide (LPS), and heat shock proteins (eHSPs) requires reliable measurement of these biomarkers that can signify exercise-induced heat stress in hot conditions. To accomplish this, both short-term (7 day) reliability (at rest, n = 12) and the acute responsiveness of each biomarker to exercise in the heat (pre and post 60-min cycling, 34.5°C and 70% RH, n = 20) were evaluated. Serum was analysed for the concentration of C-reactive protein (CRP), interleukin-6 (IL-6), heat shock protein 72 (eHSP72), immunoglobulin M (IgM) and LPS. Test–retest reliability was determined as the coefficient of variation (CV). Biomarkers with the least short-term within-participant variation were IL-6 (19%, ±20%; CV, ±95% confidence limits (CL)) and LPS (23%, ±13%). Greater variability was observed for IgM, eHSP72 and CRP (CV range 28–38%). IL-6 exhibited the largest increase in response to acute exercise (95%, ±11%, P = < 0.001) and although CRP had a modest CV (12%, ±7%), it increased substantially post-exercise (P = 0.02, ES; 0.78). In contrast, eHSP72 and LPS exhibited trivial changes post-exercise. It appears variation of common inflammatory markers after exercise in the heat is not always discernible from short-term (weekly) variation.

Original languageEnglish
Pages (from-to)1622-1628
Number of pages7
JournalJournal of Sports Sciences
Volume35
Issue number16
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Interleukin-6
Hot Temperature
HSP72 Heat-Shock Proteins
Lipopolysaccharides
C-Reactive Protein
Biomarkers
Immunoglobulin M
Heat-Shock Proteins
Protein C
Serum
Exercise
Cytokines

Cite this

Guy, Joshua H. ; Edwards, Andrew M. ; Miller, Catherine M. ; Deakin, Glen B. ; Pyne, David B. / Short-term reliability of inflammatory mediators and response to exercise in the heat. In: Journal of Sports Sciences. 2017 ; Vol. 35, No. 16. pp. 1622-1628.
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Short-term reliability of inflammatory mediators and response to exercise in the heat. / Guy, Joshua H.; Edwards, Andrew M.; Miller, Catherine M.; Deakin, Glen B.; Pyne, David B.

In: Journal of Sports Sciences, Vol. 35, No. 16, 2017, p. 1622-1628.

Research output: Contribution to journalArticle

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AU - Pyne, David B.

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