Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa

Adam J. Koppers, Geoffry N. De Iuliis, Jane M. Finnie, Eileen A. McLaughlin, R. John Aitken

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

Context: Male infertility has been linked with the excessive generation of reactive oxygen species (ROS) by defective spermatozoa. However, the subcellular origins of this activity are unclear. Objective: The objective of this study was to determine the importance of sperm mitochondria in creating the oxidative stress associated with defective sperm function. Method: Intracellular measurement of mitochondrial ROS generation and lipid peroxidation was performed using the fluorescent probes MitoSOX red and BODIPY C11 in conjunction with flow cytometry. Effects on sperm movement were measured by computer-assisted sperm analysis. Results: Disruption of mitochondrial electron transport flow in human spermatozoa resulted in generation of ROS from complex I (rotenone sensitive) or III (myxothiazol, antimycin A sensitive) via mechanisms that were independent of mitochondrial membrane potential. Activation of ROS generation at complex III led to the rapid release of hydrogen peroxide into the extracellular space, but no detectable peroxidative damage. Conversely, the induction of ROS on the matrix side of the inner mitochondrial membrane at complex I resulted in peroxidative damage to the midpiece and a loss of sperm movement that could be prevented by the concomitant presence of α-tocopherol. Defective human spermatozoa spontaneously generated mitochondrial ROS in a manner that was negatively correlated with motility. Simultaneous measurement of general cellular ROS generation with dihydroethidium indicated that 68% of the variability in such measurements could be explained by differences in mitochondrial ROS production. Conclusion: We conclude that the sperm mitochondria make a significant contribution to the oxidative stress experienced by defective human spermatozoa.

Original languageEnglish
Pages (from-to)3199-3207
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume93
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

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Oxidative stress
Spermatozoa
Reactive Oxygen Species
Oxidative Stress
Mitochondria
Antimycin A
Membranes
Rotenone
Tocopherols
Flow cytometry
Electron Transport Complex III
Fluorescent Dyes
Hydrogen Peroxide
Mitochondrial Membrane Potential
Male Infertility
Extracellular Space
Mitochondrial Membranes
Electron Transport
Chemical activation
Lipid Peroxidation

Cite this

Koppers, Adam J. ; De Iuliis, Geoffry N. ; Finnie, Jane M. ; McLaughlin, Eileen A. ; Aitken, R. John. / Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. In: Journal of Clinical Endocrinology and Metabolism. 2008 ; Vol. 93, No. 8. pp. 3199-3207.
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abstract = "Context: Male infertility has been linked with the excessive generation of reactive oxygen species (ROS) by defective spermatozoa. However, the subcellular origins of this activity are unclear. Objective: The objective of this study was to determine the importance of sperm mitochondria in creating the oxidative stress associated with defective sperm function. Method: Intracellular measurement of mitochondrial ROS generation and lipid peroxidation was performed using the fluorescent probes MitoSOX red and BODIPY C11 in conjunction with flow cytometry. Effects on sperm movement were measured by computer-assisted sperm analysis. Results: Disruption of mitochondrial electron transport flow in human spermatozoa resulted in generation of ROS from complex I (rotenone sensitive) or III (myxothiazol, antimycin A sensitive) via mechanisms that were independent of mitochondrial membrane potential. Activation of ROS generation at complex III led to the rapid release of hydrogen peroxide into the extracellular space, but no detectable peroxidative damage. Conversely, the induction of ROS on the matrix side of the inner mitochondrial membrane at complex I resulted in peroxidative damage to the midpiece and a loss of sperm movement that could be prevented by the concomitant presence of α-tocopherol. Defective human spermatozoa spontaneously generated mitochondrial ROS in a manner that was negatively correlated with motility. Simultaneous measurement of general cellular ROS generation with dihydroethidium indicated that 68{\%} of the variability in such measurements could be explained by differences in mitochondrial ROS production. Conclusion: We conclude that the sperm mitochondria make a significant contribution to the oxidative stress experienced by defective human spermatozoa.",
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Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. / Koppers, Adam J.; De Iuliis, Geoffry N.; Finnie, Jane M.; McLaughlin, Eileen A.; Aitken, R. John.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 93, No. 8, 08.2008, p. 3199-3207.

Research output: Contribution to journalArticle

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