Spectral Variations for Reaction Products Formed Between Different Amino Acids and Latent Fingermark Detection Reagents on a Range of Cellulose-Based Substrates

Xanthe Spindler, Milutin Stoilovic, Chris Lennard, Aimee Lennard

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ninhydrin, 1,2-indanedione, 1,2-indanedione-zinc, and l,8-diazafluoren-9- one (DFO) are reagents used worldwide for latent fingermark detection on paper substrates. Although research groups have concentrated on optimization studies and improvements in reagent formulations, mechanistic studies and comparisons against the different amino acid constituents in eccrine secretions are rare in the literature. It is known from studies undertaken in different geographic areas that these reagents produce varied results on different paper substrates under different environmental conditions; however, such observations have not been quantified. In this study, ninhydrin, indanedione, indanedione-zinc, and DFO reagents have been used to enhance deposits of nine major amino acids on three types of cellulose-based media: ashless filter paper, 10% recycled copy paper, and cellulose-coated TLC plates. Absorption and luminescence spectra were recorded for the resulting reaction products. The results provide some insight into the activity of these fingermark detection reagents with respect to the different amino acids present in eccrine deposits.
Original languageEnglish
Pages (from-to)308-324
Number of pages17
JournalJournal of Forensic Identification
Volume59
Issue number3
Publication statusPublished - 2009

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Cellulose
Amino Acids
Ninhydrin
Zinc
Luminescence
1,2-indanedione
Research

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title = "Spectral Variations for Reaction Products Formed Between Different Amino Acids and Latent Fingermark Detection Reagents on a Range of Cellulose-Based Substrates",
abstract = "Ninhydrin, 1,2-indanedione, 1,2-indanedione-zinc, and l,8-diazafluoren-9- one (DFO) are reagents used worldwide for latent fingermark detection on paper substrates. Although research groups have concentrated on optimization studies and improvements in reagent formulations, mechanistic studies and comparisons against the different amino acid constituents in eccrine secretions are rare in the literature. It is known from studies undertaken in different geographic areas that these reagents produce varied results on different paper substrates under different environmental conditions; however, such observations have not been quantified. In this study, ninhydrin, indanedione, indanedione-zinc, and DFO reagents have been used to enhance deposits of nine major amino acids on three types of cellulose-based media: ashless filter paper, 10{\%} recycled copy paper, and cellulose-coated TLC plates. Absorption and luminescence spectra were recorded for the resulting reaction products. The results provide some insight into the activity of these fingermark detection reagents with respect to the different amino acids present in eccrine deposits.",
author = "Xanthe Spindler and Milutin Stoilovic and Chris Lennard and Aimee Lennard",
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language = "English",
volume = "59",
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journal = "Journal of Forensic Identification",
issn = "0895-173X",
publisher = "International Association for Identification",
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Spectral Variations for Reaction Products Formed Between Different Amino Acids and Latent Fingermark Detection Reagents on a Range of Cellulose-Based Substrates. / Spindler, Xanthe; Stoilovic, Milutin; Lennard, Chris; Lennard, Aimee.

In: Journal of Forensic Identification, Vol. 59, No. 3, 2009, p. 308-324.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spectral Variations for Reaction Products Formed Between Different Amino Acids and Latent Fingermark Detection Reagents on a Range of Cellulose-Based Substrates

AU - Spindler, Xanthe

AU - Stoilovic, Milutin

AU - Lennard, Chris

AU - Lennard, Aimee

PY - 2009

Y1 - 2009

N2 - Ninhydrin, 1,2-indanedione, 1,2-indanedione-zinc, and l,8-diazafluoren-9- one (DFO) are reagents used worldwide for latent fingermark detection on paper substrates. Although research groups have concentrated on optimization studies and improvements in reagent formulations, mechanistic studies and comparisons against the different amino acid constituents in eccrine secretions are rare in the literature. It is known from studies undertaken in different geographic areas that these reagents produce varied results on different paper substrates under different environmental conditions; however, such observations have not been quantified. In this study, ninhydrin, indanedione, indanedione-zinc, and DFO reagents have been used to enhance deposits of nine major amino acids on three types of cellulose-based media: ashless filter paper, 10% recycled copy paper, and cellulose-coated TLC plates. Absorption and luminescence spectra were recorded for the resulting reaction products. The results provide some insight into the activity of these fingermark detection reagents with respect to the different amino acids present in eccrine deposits.

AB - Ninhydrin, 1,2-indanedione, 1,2-indanedione-zinc, and l,8-diazafluoren-9- one (DFO) are reagents used worldwide for latent fingermark detection on paper substrates. Although research groups have concentrated on optimization studies and improvements in reagent formulations, mechanistic studies and comparisons against the different amino acid constituents in eccrine secretions are rare in the literature. It is known from studies undertaken in different geographic areas that these reagents produce varied results on different paper substrates under different environmental conditions; however, such observations have not been quantified. In this study, ninhydrin, indanedione, indanedione-zinc, and DFO reagents have been used to enhance deposits of nine major amino acids on three types of cellulose-based media: ashless filter paper, 10% recycled copy paper, and cellulose-coated TLC plates. Absorption and luminescence spectra were recorded for the resulting reaction products. The results provide some insight into the activity of these fingermark detection reagents with respect to the different amino acids present in eccrine deposits.

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