Stable isotope analysis to detect copper (Cu) accumulation in species with high endogenous Cu concentrations: Linking Cu accumulation with toxic effects in the gastropod Bembicium nanum

    Research output: Contribution to journalArticle

    Abstract

    Tissue metal concentrations are widely used as indicators of environmental contamination. High endogenous concentrations of essential metals such as copper (Cu) can mask changes in tissue metal concentrations. Experiments using enriched isotopes can provide greater insight into endogenous Cu exchange and more broadly the exposure-bioaccumulation-response relationship. The aim of the present study was to investigate the effect of Cu exposure through the dietary uptake route in the intertidal gastropod Bembicium nanum. The marine alga Tetraselmis chuii was cultured at different Cu concentrations and fed to B. nanum. The Cu concentrations in T. chuii and B. nanum were analysed. Effects were measured using the biomarkers - total antioxidant capacity, lipid peroxidation and lysosomal membrane destabilisation in B. nanum. Dietary uptake of Cu in B. nanum was investigated with a feeding experiment using a 65Cu isotopically enriched solution. Although there were no significant differences in the Cu concentrations in B. nanum, lysosomal membrane destabilisation increased with the dietary Cu concentration. The 65Cu experiment demonstrated that B. nanum accumulated Cu under the experimental conditions. The study showed that tissue Cu concentrations are not always indicative of effects in organisms and that enriched isotope analysis assists in understanding the exposure-bioaccumulation-response relationship. © CSIRO 2017.
    Original languageEnglish
    Pages (from-to)2087-2094
    Number of pages8
    JournalMarine and Freshwater Research
    Volume68
    Issue number11
    DOIs
    Publication statusPublished - 2017

    Fingerprint

    gastropod
    stable isotopes
    Gastropoda
    stable isotope
    copper
    metals
    bioaccumulation
    algae
    metal
    isotopes
    isotope
    membrane
    uptake mechanisms
    dietary exposure
    experiment
    antioxidant
    biomarker
    biomarkers
    lipid peroxidation
    pollution

    Cite this

    @article{7bbd3bd7b2ca4a5fac7a70ec28414606,
    title = "Stable isotope analysis to detect copper (Cu) accumulation in species with high endogenous Cu concentrations: Linking Cu accumulation with toxic effects in the gastropod Bembicium nanum",
    abstract = "Tissue metal concentrations are widely used as indicators of environmental contamination. High endogenous concentrations of essential metals such as copper (Cu) can mask changes in tissue metal concentrations. Experiments using enriched isotopes can provide greater insight into endogenous Cu exchange and more broadly the exposure-bioaccumulation-response relationship. The aim of the present study was to investigate the effect of Cu exposure through the dietary uptake route in the intertidal gastropod Bembicium nanum. The marine alga Tetraselmis chuii was cultured at different Cu concentrations and fed to B. nanum. The Cu concentrations in T. chuii and B. nanum were analysed. Effects were measured using the biomarkers - total antioxidant capacity, lipid peroxidation and lysosomal membrane destabilisation in B. nanum. Dietary uptake of Cu in B. nanum was investigated with a feeding experiment using a 65Cu isotopically enriched solution. Although there were no significant differences in the Cu concentrations in B. nanum, lysosomal membrane destabilisation increased with the dietary Cu concentration. The 65Cu experiment demonstrated that B. nanum accumulated Cu under the experimental conditions. The study showed that tissue Cu concentrations are not always indicative of effects in organisms and that enriched isotope analysis assists in understanding the exposure-bioaccumulation-response relationship. {\circledC} CSIRO 2017.",
    author = "R.P. Ubrihien and A.M. Taylor and F. Krikowa and W.A. Maher",
    note = "cited By 0",
    year = "2017",
    doi = "10.1071/MF16405",
    language = "English",
    volume = "68",
    pages = "2087--2094",
    journal = "Marine Freshwater Research",
    issn = "0067-1940",
    publisher = "CSIRO",
    number = "11",

    }

    TY - JOUR

    T1 - Stable isotope analysis to detect copper (Cu) accumulation in species with high endogenous Cu concentrations: Linking Cu accumulation with toxic effects in the gastropod Bembicium nanum

    AU - Ubrihien, R.P.

    AU - Taylor, A.M.

    AU - Krikowa, F.

    AU - Maher, W.A.

    N1 - cited By 0

    PY - 2017

    Y1 - 2017

    N2 - Tissue metal concentrations are widely used as indicators of environmental contamination. High endogenous concentrations of essential metals such as copper (Cu) can mask changes in tissue metal concentrations. Experiments using enriched isotopes can provide greater insight into endogenous Cu exchange and more broadly the exposure-bioaccumulation-response relationship. The aim of the present study was to investigate the effect of Cu exposure through the dietary uptake route in the intertidal gastropod Bembicium nanum. The marine alga Tetraselmis chuii was cultured at different Cu concentrations and fed to B. nanum. The Cu concentrations in T. chuii and B. nanum were analysed. Effects were measured using the biomarkers - total antioxidant capacity, lipid peroxidation and lysosomal membrane destabilisation in B. nanum. Dietary uptake of Cu in B. nanum was investigated with a feeding experiment using a 65Cu isotopically enriched solution. Although there were no significant differences in the Cu concentrations in B. nanum, lysosomal membrane destabilisation increased with the dietary Cu concentration. The 65Cu experiment demonstrated that B. nanum accumulated Cu under the experimental conditions. The study showed that tissue Cu concentrations are not always indicative of effects in organisms and that enriched isotope analysis assists in understanding the exposure-bioaccumulation-response relationship. © CSIRO 2017.

    AB - Tissue metal concentrations are widely used as indicators of environmental contamination. High endogenous concentrations of essential metals such as copper (Cu) can mask changes in tissue metal concentrations. Experiments using enriched isotopes can provide greater insight into endogenous Cu exchange and more broadly the exposure-bioaccumulation-response relationship. The aim of the present study was to investigate the effect of Cu exposure through the dietary uptake route in the intertidal gastropod Bembicium nanum. The marine alga Tetraselmis chuii was cultured at different Cu concentrations and fed to B. nanum. The Cu concentrations in T. chuii and B. nanum were analysed. Effects were measured using the biomarkers - total antioxidant capacity, lipid peroxidation and lysosomal membrane destabilisation in B. nanum. Dietary uptake of Cu in B. nanum was investigated with a feeding experiment using a 65Cu isotopically enriched solution. Although there were no significant differences in the Cu concentrations in B. nanum, lysosomal membrane destabilisation increased with the dietary Cu concentration. The 65Cu experiment demonstrated that B. nanum accumulated Cu under the experimental conditions. The study showed that tissue Cu concentrations are not always indicative of effects in organisms and that enriched isotope analysis assists in understanding the exposure-bioaccumulation-response relationship. © CSIRO 2017.

    U2 - 10.1071/MF16405

    DO - 10.1071/MF16405

    M3 - Article

    VL - 68

    SP - 2087

    EP - 2094

    JO - Marine Freshwater Research

    JF - Marine Freshwater Research

    SN - 0067-1940

    IS - 11

    ER -