Standard methods for molecular research in Apis mellifera

Jay D. Evans, Ryan S. Schwarz, Yan Ping Chen, Giles Budge, Robert S. Cornman, Pilar De la Rua, Joachim R. de Miranda, Sylvain Foret, Leonard Foster, Laurent Gauthier, Elke Genersch, Sebastian Gisder, Antje Jarosch, Robert Kucharski, Dawn Lopez, Cheng Man Lun, Robin F. A. Moritz, Ryszard Maleszka, Irene Munoz, M. Alice Pinto

Research output: Contribution to journalReview article

68 Citations (Scopus)

Abstract

From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.
Original languageEnglish
Pages (from-to)1-54
Number of pages54
JournalJournal of Apicultural Research
Volume52
Issue number4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this

Evans, J. D., Schwarz, R. S., Chen, Y. P., Budge, G., Cornman, R. S., De la Rua, P., ... Alice Pinto, M. (2013). Standard methods for molecular research in Apis mellifera. Journal of Apicultural Research, 52(4), 1-54. https://doi.org/10.3896/IBRA.1.52.4.11
Evans, Jay D. ; Schwarz, Ryan S. ; Chen, Yan Ping ; Budge, Giles ; Cornman, Robert S. ; De la Rua, Pilar ; de Miranda, Joachim R. ; Foret, Sylvain ; Foster, Leonard ; Gauthier, Laurent ; Genersch, Elke ; Gisder, Sebastian ; Jarosch, Antje ; Kucharski, Robert ; Lopez, Dawn ; Lun, Cheng Man ; Moritz, Robin F. A. ; Maleszka, Ryszard ; Munoz, Irene ; Alice Pinto, M. / Standard methods for molecular research in Apis mellifera. In: Journal of Apicultural Research. 2013 ; Vol. 52, No. 4. pp. 1-54.
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abstract = "From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.",
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Evans, JD, Schwarz, RS, Chen, YP, Budge, G, Cornman, RS, De la Rua, P, de Miranda, JR, Foret, S, Foster, L, Gauthier, L, Genersch, E, Gisder, S, Jarosch, A, Kucharski, R, Lopez, D, Lun, CM, Moritz, RFA, Maleszka, R, Munoz, I & Alice Pinto, M 2013, 'Standard methods for molecular research in Apis mellifera', Journal of Apicultural Research, vol. 52, no. 4, pp. 1-54. https://doi.org/10.3896/IBRA.1.52.4.11

Standard methods for molecular research in Apis mellifera. / Evans, Jay D.; Schwarz, Ryan S.; Chen, Yan Ping; Budge, Giles; Cornman, Robert S.; De la Rua, Pilar; de Miranda, Joachim R.; Foret, Sylvain; Foster, Leonard; Gauthier, Laurent; Genersch, Elke; Gisder, Sebastian; Jarosch, Antje; Kucharski, Robert; Lopez, Dawn; Lun, Cheng Man; Moritz, Robin F. A.; Maleszka, Ryszard; Munoz, Irene; Alice Pinto, M.

In: Journal of Apicultural Research, Vol. 52, No. 4, 2013, p. 1-54.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Standard methods for molecular research in Apis mellifera

AU - Evans, Jay D.

AU - Schwarz, Ryan S.

AU - Chen, Yan Ping

AU - Budge, Giles

AU - Cornman, Robert S.

AU - De la Rua, Pilar

AU - de Miranda, Joachim R.

AU - Foret, Sylvain

AU - Foster, Leonard

AU - Gauthier, Laurent

AU - Genersch, Elke

AU - Gisder, Sebastian

AU - Jarosch, Antje

AU - Kucharski, Robert

AU - Lopez, Dawn

AU - Lun, Cheng Man

AU - Moritz, Robin F. A.

AU - Maleszka, Ryszard

AU - Munoz, Irene

AU - Alice Pinto, M.

PY - 2013

Y1 - 2013

N2 - From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.

AB - From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.

KW - Apis mellifera

KW - BEEBOOK

KW - Colony Collapse Disorder

KW - COLOSS

KW - Development

KW - Disease

KW - DNA extraction

KW - Genomic

KW - Honey bee

KW - In situ Hybridization

KW - Methylation

KW - Microsatellite

KW - Mitochondrial DNA

KW - Next-generation sequencing

KW - Northern Blot

KW - Pollination

KW - Population genetics

KW - Quantitative PCR

KW - RNA interference

KW - RNAi

KW - Southern Blot

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U2 - 10.3896/IBRA.1.52.4.11

DO - 10.3896/IBRA.1.52.4.11

M3 - Review article

VL - 52

SP - 1

EP - 54

JO - Journal of Apicultural Research

JF - Journal of Apicultural Research

SN - 0005-772X

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Evans JD, Schwarz RS, Chen YP, Budge G, Cornman RS, De la Rua P et al. Standard methods for molecular research in Apis mellifera. Journal of Apicultural Research. 2013;52(4):1-54. https://doi.org/10.3896/IBRA.1.52.4.11