STR genotyping of exogenous hair shat DNA

Dennis McNevin

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Most hairs found at crime scenes yield low quality and/or low quantities of nuclear DNA. This DNA is further depleted when stringent hair cleaning procedures are applied in the laboratory, suggesting that detectable DNA exists exogenously. The phenomenon of exogenous hair DNA is the subject of this study. DNA was extracted from washed and unwashed hairs and the resulting Profiler™ Plus STR genotypes were compared with those of reference (buccal) swabs from the hair donors. The DNA extraction procedure involved no prior cleaning of the hair sample and no dissolution of the hair during digestion, in contrast to standard procedures. The STR genotyping success was measured by recording the two dominant alleles at each locus and comparing them with the reference DNA profile. The effect of hair cleanliness was examined by leaving donors’ hair unwashed for periods of 1, 3 and 7 days before sampling. It was found that the genotyping success for unwashed hair was significantly higher than that for freshly washed hair, with the majority of clean hair samples producing little or no DNA. Genotyping success was also lower for donors with cosmetically treated hair compared with those having untreated hair. Although the quality of STR profiles (i.e. allele dropout, differential amplification) from hair shafts or telogen hair clubs is reduced compared with those from other biological sources, the genotypes obtained in this study may be usable and are certainly discriminating if alternative interpretational methods are applied
    Original languageEnglish
    Pages (from-to)107-122
    Number of pages16
    JournalAustralian Journal of Forensic Sciences
    Volume39
    Issue number2
    DOIs
    Publication statusPublished - 2007

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    Hair
    DNA
    Alleles
    Genotype
    Cheek
    Crime
    Digestion

    Cite this

    McNevin, Dennis. / STR genotyping of exogenous hair shat DNA. In: Australian Journal of Forensic Sciences. 2007 ; Vol. 39, No. 2. pp. 107-122.
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    title = "STR genotyping of exogenous hair shat DNA",
    abstract = "Most hairs found at crime scenes yield low quality and/or low quantities of nuclear DNA. This DNA is further depleted when stringent hair cleaning procedures are applied in the laboratory, suggesting that detectable DNA exists exogenously. The phenomenon of exogenous hair DNA is the subject of this study. DNA was extracted from washed and unwashed hairs and the resulting Profiler™ Plus STR genotypes were compared with those of reference (buccal) swabs from the hair donors. The DNA extraction procedure involved no prior cleaning of the hair sample and no dissolution of the hair during digestion, in contrast to standard procedures. The STR genotyping success was measured by recording the two dominant alleles at each locus and comparing them with the reference DNA profile. The effect of hair cleanliness was examined by leaving donors’ hair unwashed for periods of 1, 3 and 7 days before sampling. It was found that the genotyping success for unwashed hair was significantly higher than that for freshly washed hair, with the majority of clean hair samples producing little or no DNA. Genotyping success was also lower for donors with cosmetically treated hair compared with those having untreated hair. Although the quality of STR profiles (i.e. allele dropout, differential amplification) from hair shafts or telogen hair clubs is reduced compared with those from other biological sources, the genotypes obtained in this study may be usable and are certainly discriminating if alternative interpretational methods are applied",
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    journal = "Australian Journal of Forensic Sciences",
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    STR genotyping of exogenous hair shat DNA. / McNevin, Dennis.

    In: Australian Journal of Forensic Sciences, Vol. 39, No. 2, 2007, p. 107-122.

    Research output: Contribution to journalArticle

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    N2 - Most hairs found at crime scenes yield low quality and/or low quantities of nuclear DNA. This DNA is further depleted when stringent hair cleaning procedures are applied in the laboratory, suggesting that detectable DNA exists exogenously. The phenomenon of exogenous hair DNA is the subject of this study. DNA was extracted from washed and unwashed hairs and the resulting Profiler™ Plus STR genotypes were compared with those of reference (buccal) swabs from the hair donors. The DNA extraction procedure involved no prior cleaning of the hair sample and no dissolution of the hair during digestion, in contrast to standard procedures. The STR genotyping success was measured by recording the two dominant alleles at each locus and comparing them with the reference DNA profile. The effect of hair cleanliness was examined by leaving donors’ hair unwashed for periods of 1, 3 and 7 days before sampling. It was found that the genotyping success for unwashed hair was significantly higher than that for freshly washed hair, with the majority of clean hair samples producing little or no DNA. Genotyping success was also lower for donors with cosmetically treated hair compared with those having untreated hair. Although the quality of STR profiles (i.e. allele dropout, differential amplification) from hair shafts or telogen hair clubs is reduced compared with those from other biological sources, the genotypes obtained in this study may be usable and are certainly discriminating if alternative interpretational methods are applied

    AB - Most hairs found at crime scenes yield low quality and/or low quantities of nuclear DNA. This DNA is further depleted when stringent hair cleaning procedures are applied in the laboratory, suggesting that detectable DNA exists exogenously. The phenomenon of exogenous hair DNA is the subject of this study. DNA was extracted from washed and unwashed hairs and the resulting Profiler™ Plus STR genotypes were compared with those of reference (buccal) swabs from the hair donors. The DNA extraction procedure involved no prior cleaning of the hair sample and no dissolution of the hair during digestion, in contrast to standard procedures. The STR genotyping success was measured by recording the two dominant alleles at each locus and comparing them with the reference DNA profile. The effect of hair cleanliness was examined by leaving donors’ hair unwashed for periods of 1, 3 and 7 days before sampling. It was found that the genotyping success for unwashed hair was significantly higher than that for freshly washed hair, with the majority of clean hair samples producing little or no DNA. Genotyping success was also lower for donors with cosmetically treated hair compared with those having untreated hair. Although the quality of STR profiles (i.e. allele dropout, differential amplification) from hair shafts or telogen hair clubs is reduced compared with those from other biological sources, the genotypes obtained in this study may be usable and are certainly discriminating if alternative interpretational methods are applied

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