Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

Aisling Smyth; Martina Gogarty; Daniel Crean; Evelyn P Murphy

doi:10.1007/978-1-4939-9195-2_3

Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

Aisling Smyth, Martina Gogarty, Daniel Crean, Evelyn P Murphy

Research output: A Conference proceeding or a Chapter in Book › Chapter › peer-review

Abstract

NR4A1-3 receptors are required in inflammatory disease initiation and progression, where they function as early response regulators, controlling the extent of the inflammatory response and promoting inflammatory resolution. NR4A receptor activity controls inflammatory processes in several diseases characterized by chronic inflammation including rheumatoid arthritis (RA) and atherosclerosis. Studies indicate that cell-type and cellular microenvironment can alter NR4A1-3 receptor activity and influence their biological roles. Thus, the study of appropriate in vivo models of inflammatory disease is important to ascertain their cell- and tissue-specific functional roles. Here we describe immunohistochemical approaches optimized to study the expression patterns of NR4A nuclear receptors in inflamed synovium tissues obtained from patients diagnosed with RA and mouse models of inflammatory joint disease.

Original language	English
Title of host publication	Methods in Molecular Biology
Subtitle of host publication	Methods and Experimental Protocols
Editors	Mostafa Z.Badr
Place of Publication	United States
Publisher	Humana New York
Chapter	3
Pages	17-26
Number of pages	10
Edition	1
ISBN (Electronic)	9781493991952
ISBN (Print)	9781493991945
DOIs	https://doi.org/10.1007/978-1-4939-9195-2_3
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1966
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-4939-9195-2_3

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Smyth, A., Gogarty, M., Crean, D., & Murphy, E. P. (2019). Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue. In M. Z.Badr (Ed.), Methods in Molecular Biology: Methods and Experimental Protocols (1 ed., pp. 17-26). (Methods in Molecular Biology; Vol. 1966). Humana New York. https://doi.org/10.1007/978-1-4939-9195-2_3

@inbook{87a02c9572c84ecf811d4e138334fc49,

title = "Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue",

abstract = "NR4A1-3 receptors are required in inflammatory disease initiation and progression, where they function as early response regulators, controlling the extent of the inflammatory response and promoting inflammatory resolution. NR4A receptor activity controls inflammatory processes in several diseases characterized by chronic inflammation including rheumatoid arthritis (RA) and atherosclerosis. Studies indicate that cell-type and cellular microenvironment can alter NR4A1-3 receptor activity and influence their biological roles. Thus, the study of appropriate in vivo models of inflammatory disease is important to ascertain their cell- and tissue-specific functional roles. Here we describe immunohistochemical approaches optimized to study the expression patterns of NR4A nuclear receptors in inflamed synovium tissues obtained from patients diagnosed with RA and mouse models of inflammatory joint disease.",

keywords = "Animals, Arthritis, Rheumatoid/metabolism, Disease Models, Animal, Humans, Immunohistochemistry/methods, Mice, Nuclear Receptor Subfamily 4, Group A, Member 2/analysis, Protein Transport, Synovial Membrane/metabolism, NR4A nuclear receptors, Immunohistochemical analysis, Rheumatoid arthritis, Synovial joint tissue, Immunofluorescence, TNF mouse model",

author = "Aisling Smyth and Martina Gogarty and Daniel Crean and Murphy, {Evelyn P}",

note = "Funding Information: This work was supported by Science Foundation Ireland funding (IN.1B2613) to E.P.M. Whole ankle joints from TNF-ΔARE mice and na{\"i}ve mice were kindly provided by Dr. Carol Aherne, School of Medicine, University of Colorado, Denver, USA. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",

year = "2019",

doi = "10.1007/978-1-4939-9195-2_3",

language = "English",

isbn = "9781493991945",

series = "Methods in Molecular Biology",

publisher = "Humana New York",

pages = "17--26",

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booktitle = "Methods in Molecular Biology",

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}

Smyth, A, Gogarty, M, Crean, D & Murphy, EP 2019, Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue. in M Z.Badr (ed.), Methods in Molecular Biology: Methods and Experimental Protocols. 1 edn, Methods in Molecular Biology, vol. 1966, Humana New York, United States, pp. 17-26. https://doi.org/10.1007/978-1-4939-9195-2_3

Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue. / Smyth, Aisling; Gogarty, Martina; Crean, Daniel et al.
Methods in Molecular Biology: Methods and Experimental Protocols. ed. / Mostafa Z.Badr. 1. ed. United States: Humana New York, 2019. p. 17-26 (Methods in Molecular Biology; Vol. 1966).

Research output: A Conference proceeding or a Chapter in Book › Chapter › peer-review

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T1 - Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

AU - Smyth, Aisling

AU - Gogarty, Martina

AU - Crean, Daniel

AU - Murphy, Evelyn P

N1 - Funding Information: This work was supported by Science Foundation Ireland funding (IN.1B2613) to E.P.M. Whole ankle joints from TNF-ΔARE mice and naïve mice were kindly provided by Dr. Carol Aherne, School of Medicine, University of Colorado, Denver, USA. Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - NR4A1-3 receptors are required in inflammatory disease initiation and progression, where they function as early response regulators, controlling the extent of the inflammatory response and promoting inflammatory resolution. NR4A receptor activity controls inflammatory processes in several diseases characterized by chronic inflammation including rheumatoid arthritis (RA) and atherosclerosis. Studies indicate that cell-type and cellular microenvironment can alter NR4A1-3 receptor activity and influence their biological roles. Thus, the study of appropriate in vivo models of inflammatory disease is important to ascertain their cell- and tissue-specific functional roles. Here we describe immunohistochemical approaches optimized to study the expression patterns of NR4A nuclear receptors in inflamed synovium tissues obtained from patients diagnosed with RA and mouse models of inflammatory joint disease.

AB - NR4A1-3 receptors are required in inflammatory disease initiation and progression, where they function as early response regulators, controlling the extent of the inflammatory response and promoting inflammatory resolution. NR4A receptor activity controls inflammatory processes in several diseases characterized by chronic inflammation including rheumatoid arthritis (RA) and atherosclerosis. Studies indicate that cell-type and cellular microenvironment can alter NR4A1-3 receptor activity and influence their biological roles. Thus, the study of appropriate in vivo models of inflammatory disease is important to ascertain their cell- and tissue-specific functional roles. Here we describe immunohistochemical approaches optimized to study the expression patterns of NR4A nuclear receptors in inflamed synovium tissues obtained from patients diagnosed with RA and mouse models of inflammatory joint disease.

KW - Animals

KW - Arthritis, Rheumatoid/metabolism

KW - Disease Models, Animal

KW - Humans

KW - Immunohistochemistry/methods

KW - Mice

KW - Nuclear Receptor Subfamily 4, Group A, Member 2/analysis

KW - Protein Transport

KW - Synovial Membrane/metabolism

KW - NR4A nuclear receptors

KW - Immunohistochemical analysis

KW - Rheumatoid arthritis

KW - Synovial joint tissue

KW - Immunofluorescence

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DO - 10.1007/978-1-4939-9195-2_3

M3 - Chapter

C2 - 31041736

SN - 9781493991945

T3 - Methods in Molecular Biology

SP - 17

EP - 26

BT - Methods in Molecular Biology

A2 - Z.Badr, Mostafa

PB - Humana New York

CY - United States

ER -

Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

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