Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness

Nicholas P. West, Peggy L. Horn, Susan Barrett, Hilary S. Warren, Markus J. Lehtinen, Gus Koerbin, Mary Brun, David B. Pyne, Sampo J. Lahtinen, Peter A. Fricker, Allan W. Cripps

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and aims: The immune mechanisms by which probiotics reduce susceptibility to upper respiratory tract illness is uncertain. The aim of this study was to examine purported cell-mediated immune mechanisms that might explain the reduction in respiratory illness observed following daily supplementation with Bifidobacterium animalis subsp. lactis Bl-04 (Bl-04) and a combined Lactobacillus acidophilus NCFM & B. animalis subsp. lactis BI-07 (NCFM & Bi-07). Methods: A cohort of 144 healthy physically active individuals were allocated to daily supplementation consumed as a beverage with Bl-04 (n=46) supplemented at a dosage of 2.0×109 colony forming units (cfu) per day, NCFM & Bi-07 (n=47) at a dosage of 5.0×109CFU per day each, or a placebo (n=51) over 150d. Markers included plasma cytokines, metalloproteinases and neurotrophins, peripheral blood leucocyte numbers, antibody-dependent and antibody-independent NK cell activity (NKCA), and peripheral blood mononuclear cell (PBMC) phagocytosis. Results: A total of 125 subjects were included in the final analysis. No significant effects were observed on cytokines, on white cell differentials, NKCA or PBMC phagocytosis from pre- to post-supplementation. The biomarkers that increased significantly from pre- to post-supplementation were the concentration of plasma macrophage inflammatory protein (MIP)-1δ which was higher in the Bl-04 than placebo group (Bl-04 25%±11%, placebo-3.3%±9.4%; mean±SD, P=0.003) while the concentration of plasma matrix metallo-proteinase (MMP)-1 decreased by 11%±16% in the NCFM & Bi-07 group and increased by 21%±17% in the placebo group, which was a significant 26% difference (8-41%; P=0.02). Conclusion: Probiotic supplementation had little effect on parameters of the innate immune system. Mechanisms explaining the beneficial effect of Bl-04 or NCFM & Bi-07 supplementation on respiratory illness remain unclear.

Original languageEnglish
Pages (from-to)e178-e184
Journale-SPEN Journal
Volume9
Issue number5
DOIs
Publication statusPublished - 2014
Externally publishedYes

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Probiotics
Immune System
Placebos
Cytophagocytosis
Natural Killer Cells
Blood Cells
Cytokines
Lactobacillus acidophilus
Macrophage Inflammatory Proteins
Antibodies
Beverages
Nerve Growth Factors
Metalloproteases
Leukocyte Count
Respiratory System
Blood Proteins
Peptide Hydrolases
Stem Cells
Biomarkers
Bifidobacterium animalis

Cite this

West, N. P., Horn, P. L., Barrett, S., Warren, H. S., Lehtinen, M. J., Koerbin, G., ... Cripps, A. W. (2014). Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness. e-SPEN Journal, 9(5), e178-e184. https://doi.org/10.1016/j.clnme.2014.06.003
West, Nicholas P. ; Horn, Peggy L. ; Barrett, Susan ; Warren, Hilary S. ; Lehtinen, Markus J. ; Koerbin, Gus ; Brun, Mary ; Pyne, David B. ; Lahtinen, Sampo J. ; Fricker, Peter A. ; Cripps, Allan W. / Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness. In: e-SPEN Journal. 2014 ; Vol. 9, No. 5. pp. e178-e184.
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title = "Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness",
abstract = "Background and aims: The immune mechanisms by which probiotics reduce susceptibility to upper respiratory tract illness is uncertain. The aim of this study was to examine purported cell-mediated immune mechanisms that might explain the reduction in respiratory illness observed following daily supplementation with Bifidobacterium animalis subsp. lactis Bl-04 (Bl-04) and a combined Lactobacillus acidophilus NCFM & B. animalis subsp. lactis BI-07 (NCFM & Bi-07). Methods: A cohort of 144 healthy physically active individuals were allocated to daily supplementation consumed as a beverage with Bl-04 (n=46) supplemented at a dosage of 2.0×109 colony forming units (cfu) per day, NCFM & Bi-07 (n=47) at a dosage of 5.0×109CFU per day each, or a placebo (n=51) over 150d. Markers included plasma cytokines, metalloproteinases and neurotrophins, peripheral blood leucocyte numbers, antibody-dependent and antibody-independent NK cell activity (NKCA), and peripheral blood mononuclear cell (PBMC) phagocytosis. Results: A total of 125 subjects were included in the final analysis. No significant effects were observed on cytokines, on white cell differentials, NKCA or PBMC phagocytosis from pre- to post-supplementation. The biomarkers that increased significantly from pre- to post-supplementation were the concentration of plasma macrophage inflammatory protein (MIP)-1δ which was higher in the Bl-04 than placebo group (Bl-04 25{\%}±11{\%}, placebo-3.3{\%}±9.4{\%}; mean±SD, P=0.003) while the concentration of plasma matrix metallo-proteinase (MMP)-1 decreased by 11{\%}±16{\%} in the NCFM & Bi-07 group and increased by 21{\%}±17{\%} in the placebo group, which was a significant 26{\%} difference (8-41{\%}; P=0.02). Conclusion: Probiotic supplementation had little effect on parameters of the innate immune system. Mechanisms explaining the beneficial effect of Bl-04 or NCFM & Bi-07 supplementation on respiratory illness remain unclear.",
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author = "West, {Nicholas P.} and Horn, {Peggy L.} and Susan Barrett and Warren, {Hilary S.} and Lehtinen, {Markus J.} and Gus Koerbin and Mary Brun and Pyne, {David B.} and Lahtinen, {Sampo J.} and Fricker, {Peter A.} and Cripps, {Allan W.}",
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West, NP, Horn, PL, Barrett, S, Warren, HS, Lehtinen, MJ, Koerbin, G, Brun, M, Pyne, DB, Lahtinen, SJ, Fricker, PA & Cripps, AW 2014, 'Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness', e-SPEN Journal, vol. 9, no. 5, pp. e178-e184. https://doi.org/10.1016/j.clnme.2014.06.003

Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness. / West, Nicholas P.; Horn, Peggy L.; Barrett, Susan; Warren, Hilary S.; Lehtinen, Markus J.; Koerbin, Gus; Brun, Mary; Pyne, David B.; Lahtinen, Sampo J.; Fricker, Peter A.; Cripps, Allan W.

In: e-SPEN Journal, Vol. 9, No. 5, 2014, p. e178-e184.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Supplementation with a single and double strain probiotic on the innate immune system for respiratory illness

AU - West, Nicholas P.

AU - Horn, Peggy L.

AU - Barrett, Susan

AU - Warren, Hilary S.

AU - Lehtinen, Markus J.

AU - Koerbin, Gus

AU - Brun, Mary

AU - Pyne, David B.

AU - Lahtinen, Sampo J.

AU - Fricker, Peter A.

AU - Cripps, Allan W.

PY - 2014

Y1 - 2014

N2 - Background and aims: The immune mechanisms by which probiotics reduce susceptibility to upper respiratory tract illness is uncertain. The aim of this study was to examine purported cell-mediated immune mechanisms that might explain the reduction in respiratory illness observed following daily supplementation with Bifidobacterium animalis subsp. lactis Bl-04 (Bl-04) and a combined Lactobacillus acidophilus NCFM & B. animalis subsp. lactis BI-07 (NCFM & Bi-07). Methods: A cohort of 144 healthy physically active individuals were allocated to daily supplementation consumed as a beverage with Bl-04 (n=46) supplemented at a dosage of 2.0×109 colony forming units (cfu) per day, NCFM & Bi-07 (n=47) at a dosage of 5.0×109CFU per day each, or a placebo (n=51) over 150d. Markers included plasma cytokines, metalloproteinases and neurotrophins, peripheral blood leucocyte numbers, antibody-dependent and antibody-independent NK cell activity (NKCA), and peripheral blood mononuclear cell (PBMC) phagocytosis. Results: A total of 125 subjects were included in the final analysis. No significant effects were observed on cytokines, on white cell differentials, NKCA or PBMC phagocytosis from pre- to post-supplementation. The biomarkers that increased significantly from pre- to post-supplementation were the concentration of plasma macrophage inflammatory protein (MIP)-1δ which was higher in the Bl-04 than placebo group (Bl-04 25%±11%, placebo-3.3%±9.4%; mean±SD, P=0.003) while the concentration of plasma matrix metallo-proteinase (MMP)-1 decreased by 11%±16% in the NCFM & Bi-07 group and increased by 21%±17% in the placebo group, which was a significant 26% difference (8-41%; P=0.02). Conclusion: Probiotic supplementation had little effect on parameters of the innate immune system. Mechanisms explaining the beneficial effect of Bl-04 or NCFM & Bi-07 supplementation on respiratory illness remain unclear.

AB - Background and aims: The immune mechanisms by which probiotics reduce susceptibility to upper respiratory tract illness is uncertain. The aim of this study was to examine purported cell-mediated immune mechanisms that might explain the reduction in respiratory illness observed following daily supplementation with Bifidobacterium animalis subsp. lactis Bl-04 (Bl-04) and a combined Lactobacillus acidophilus NCFM & B. animalis subsp. lactis BI-07 (NCFM & Bi-07). Methods: A cohort of 144 healthy physically active individuals were allocated to daily supplementation consumed as a beverage with Bl-04 (n=46) supplemented at a dosage of 2.0×109 colony forming units (cfu) per day, NCFM & Bi-07 (n=47) at a dosage of 5.0×109CFU per day each, or a placebo (n=51) over 150d. Markers included plasma cytokines, metalloproteinases and neurotrophins, peripheral blood leucocyte numbers, antibody-dependent and antibody-independent NK cell activity (NKCA), and peripheral blood mononuclear cell (PBMC) phagocytosis. Results: A total of 125 subjects were included in the final analysis. No significant effects were observed on cytokines, on white cell differentials, NKCA or PBMC phagocytosis from pre- to post-supplementation. The biomarkers that increased significantly from pre- to post-supplementation were the concentration of plasma macrophage inflammatory protein (MIP)-1δ which was higher in the Bl-04 than placebo group (Bl-04 25%±11%, placebo-3.3%±9.4%; mean±SD, P=0.003) while the concentration of plasma matrix metallo-proteinase (MMP)-1 decreased by 11%±16% in the NCFM & Bi-07 group and increased by 21%±17% in the placebo group, which was a significant 26% difference (8-41%; P=0.02). Conclusion: Probiotic supplementation had little effect on parameters of the innate immune system. Mechanisms explaining the beneficial effect of Bl-04 or NCFM & Bi-07 supplementation on respiratory illness remain unclear.

KW - B. animalis subsp. lactis Bi-07

KW - Bifidobacterium animalis subsp. lactis Bl-04

KW - Healthy adults

KW - Innate immune

KW - Lactobacillus acidophilus NCFM

KW - Probiotics

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U2 - 10.1016/j.clnme.2014.06.003

DO - 10.1016/j.clnme.2014.06.003

M3 - Article

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SP - e178-e184

JO - e-SPEN

JF - e-SPEN

SN - 1751-4991

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ER -