The Amazonian Red Side-Necked Turtle Rhinemys rufipes (Spix, 1824) (Testudines, Chelidae) Has a GSD Sex-Determining Mechanism with an Ancient XY Sex Microchromosome System

Patrik F. Viana; Eliana Feldberg; Marcelo B. Cioffi; Vinicius Tadeu de Carvalho; Sabrina Menezes; Richard C. Vogt; Thomas Liehr; Tariq Ezaz

doi:10.3390/cells9092088

The Amazonian Red Side-Necked Turtle Rhinemys rufipes (Spix, 1824) (Testudines, Chelidae) Has a GSD Sex-Determining Mechanism with an Ancient XY Sex Microchromosome System

Patrik F. Viana, Eliana Feldberg, Marcelo B. Cioffi, Vinicius Tadeu de Carvalho, Sabrina Menezes, Richard C. Vogt, Thomas Liehr, Tariq Ezaz

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Abstract

The Amazonian red side-necked turtle Rhynemis rufipes is an endemic Amazonian Chelidae species that occurs in small streams throughout Colombia and Brazil river basins. Little is known about various biological aspects of this species, including its sex determination strategies. Among chelids, the greatest karyotype diversity is found in the Neotropical species, with several 2n configurations, including cases of triploidy. Here, we investigate the karyotype of Rhinemys rufipes by applying combined conventional and molecular cytogenetic procedures. This allowed us to discover a genetic sex-determining mechanism that shares an ancestral micro XY sex chromosome system. This ancient micro XY system recruited distinct repeat motifs before it diverged from several South America and Australasian species. We propose that such a system dates back to the earliest lineages of the chelid species before the split of South America and Australasian lineages.

Original language	English
Article number	2071
Pages (from-to)	1-15
Number of pages	15
Journal	Cells
Volume	9
Issue number	9
DOIs	https://doi.org/10.3390/cells9092088
Publication status	Published - 12 Sept 2020

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10.3390/cells9092088Licence: CC BY

ArticleFinal published version, 2.38 MBLicence: CC BY

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title = "The Amazonian Red Side-Necked Turtle Rhinemys rufipes (Spix, 1824) (Testudines, Chelidae) Has a GSD Sex-Determining Mechanism with an Ancient XY Sex Microchromosome System",

abstract = "The Amazonian red side-necked turtle Rhynemis rufipes is an endemic Amazonian Chelidae species that occurs in small streams throughout Colombia and Brazil river basins. Little is known about various biological aspects of this species, including its sex determination strategies. Among chelids, the greatest karyotype diversity is found in the Neotropical species, with several 2n configurations, including cases of triploidy. Here, we investigate the karyotype of Rhinemys rufipes by applying combined conventional and molecular cytogenetic procedures. This allowed us to discover a genetic sex-determining mechanism that shares an ancestral micro XY sex chromosome system. This ancient micro XY system recruited distinct repeat motifs before it diverged from several South America and Australasian species. We propose that such a system dates back to the earliest lineages of the chelid species before the split of South America and Australasian lineages.",

keywords = "Chelidae, comparative genome hybridization, genetic sex determination, karyotype, Neotropical region",

author = "Viana, {Patrik F.} and Eliana Feldberg and Cioffi, {Marcelo B.} and {de Carvalho}, {Vinicius Tadeu} and Sabrina Menezes and Vogt, {Richard C.} and Thomas Liehr and Tariq Ezaz",

year = "2020",

month = sep,

day = "12",

doi = "10.3390/cells9092088",

language = "English",

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journal = "Cells",

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T1 - The Amazonian Red Side-Necked Turtle Rhinemys rufipes (Spix, 1824) (Testudines, Chelidae) Has a GSD Sex-Determining Mechanism with an Ancient XY Sex Microchromosome System

AU - Viana, Patrik F.

AU - Feldberg, Eliana

AU - Cioffi, Marcelo B.

AU - de Carvalho, Vinicius Tadeu

AU - Menezes, Sabrina

AU - Vogt, Richard C.

AU - Liehr, Thomas

AU - Ezaz, Tariq

PY - 2020/9/12

Y1 - 2020/9/12

N2 - The Amazonian red side-necked turtle Rhynemis rufipes is an endemic Amazonian Chelidae species that occurs in small streams throughout Colombia and Brazil river basins. Little is known about various biological aspects of this species, including its sex determination strategies. Among chelids, the greatest karyotype diversity is found in the Neotropical species, with several 2n configurations, including cases of triploidy. Here, we investigate the karyotype of Rhinemys rufipes by applying combined conventional and molecular cytogenetic procedures. This allowed us to discover a genetic sex-determining mechanism that shares an ancestral micro XY sex chromosome system. This ancient micro XY system recruited distinct repeat motifs before it diverged from several South America and Australasian species. We propose that such a system dates back to the earliest lineages of the chelid species before the split of South America and Australasian lineages.

AB - The Amazonian red side-necked turtle Rhynemis rufipes is an endemic Amazonian Chelidae species that occurs in small streams throughout Colombia and Brazil river basins. Little is known about various biological aspects of this species, including its sex determination strategies. Among chelids, the greatest karyotype diversity is found in the Neotropical species, with several 2n configurations, including cases of triploidy. Here, we investigate the karyotype of Rhinemys rufipes by applying combined conventional and molecular cytogenetic procedures. This allowed us to discover a genetic sex-determining mechanism that shares an ancestral micro XY sex chromosome system. This ancient micro XY system recruited distinct repeat motifs before it diverged from several South America and Australasian species. We propose that such a system dates back to the earliest lineages of the chelid species before the split of South America and Australasian lineages.

KW - Chelidae

KW - comparative genome hybridization

KW - genetic sex determination

KW - karyotype

KW - Neotropical region

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AN - SCOPUS:85091053915

SN - 2073-4409

VL - 9

SP - 1

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JO - Cells

JF - Cells

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M1 - 2071

ER -

The Amazonian Red Side-Necked Turtle Rhinemys rufipes (Spix, 1824) (Testudines, Chelidae) Has a GSD Sex-Determining Mechanism with an Ancient XY Sex Microchromosome System

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