Eco-industrial parks (EIPs) provide a basis for applying the concept of industrial ecology. In Australia, eco-industrial parks are new and are being considered by state governments as a way of achieving more sustainable industrial development. However, for many firms, local government and communities, industrial ecology is a concept that is misunderstood and treated with suspicion. This paper uses research undertaken to investigate the application of industrial ecology to ensure more sustainable industry development in the State of Queensland, Australia, to develop a set of principles and planning guidelines for use by governments to facilitate the development of eco-industrial parks. Synergy Park, 22 km west of Brisbane, is Australia’s first planned eco-industrial park to be built. The planning and development of this eco-industrial park was a significant challenge to the developer and public agencies concerned with this project. The case study presented in this document provides useful lessons and advice for planners and developers involved with the planning and development of eco-industrial parks.