The Candida Albicans CaACE2 Gene Affects Morphogenesis, Adherence and Virulence

Mary T. Kelly, Donna MacCallum, Susanne Clancy, Frank C. Odds, Alistar Brown, Geraldine Butler

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Morphogenesis between yeast and hyphal growth is a characteristic associated with virulence in Candida albicans and involves changes in the cell wall. In Saccharomyces cerevisiae, the transcription factor pair Ace2p and Swi5p are key regulators of cell wall metabolism. Here, we have characterized the CaACE2 gene, which encodes the only C. albicans homologue of S. cerevisiae ACE2 and SWI5. Deleting CaACE2 results in a defect in cell separation, increased invasion of solid agar medium and inappropriate pseudohyphal growth, even in the absence of external inducers. The mutant cells have reduced adherence to plastic surfaces and generate biofilms with distinctly different morphology from wild-type cells. They are also avirulent in a mouse model. Deleting CaACE2 has no effect on expression of the chitinase gene CHT2, but expression of CHT3 and the putative cell wall genes CaDSE1 and CaSCW11 is reduced in both yeast and hyphal forms. The CaAce2 protein is localized to the daughter nucleus of large budded cells at the end of mitosis. C. albicans Ace2p therefore plays a major role in morphogenesis and adherence and resembles S. cerevisiae Ace2p in function
Original languageEnglish
Pages (from-to)969-983
Number of pages15
JournalMolecular Microbiology
Volume53
Issue number3
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Candida albicans
Morphogenesis
Cell Wall
Virulence
Saccharomyces cerevisiae
Yeasts
Genes
Chitinases
Cell Separation
Biofilms
Growth
Mitosis
Plastics
Agar
Transcription Factors
Gene Expression
Proteins

Cite this

Kelly, M. T., MacCallum, D., Clancy, S., Odds, F. C., Brown, A., & Butler, G. (2004). The Candida Albicans CaACE2 Gene Affects Morphogenesis, Adherence and Virulence. Molecular Microbiology, 53(3), 969-983. https://doi.org/10.1111/j.1365-2958.2004.04185.x
Kelly, Mary T. ; MacCallum, Donna ; Clancy, Susanne ; Odds, Frank C. ; Brown, Alistar ; Butler, Geraldine. / The Candida Albicans CaACE2 Gene Affects Morphogenesis, Adherence and Virulence. In: Molecular Microbiology. 2004 ; Vol. 53, No. 3. pp. 969-983.
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abstract = "Morphogenesis between yeast and hyphal growth is a characteristic associated with virulence in Candida albicans and involves changes in the cell wall. In Saccharomyces cerevisiae, the transcription factor pair Ace2p and Swi5p are key regulators of cell wall metabolism. Here, we have characterized the CaACE2 gene, which encodes the only C. albicans homologue of S. cerevisiae ACE2 and SWI5. Deleting CaACE2 results in a defect in cell separation, increased invasion of solid agar medium and inappropriate pseudohyphal growth, even in the absence of external inducers. The mutant cells have reduced adherence to plastic surfaces and generate biofilms with distinctly different morphology from wild-type cells. They are also avirulent in a mouse model. Deleting CaACE2 has no effect on expression of the chitinase gene CHT2, but expression of CHT3 and the putative cell wall genes CaDSE1 and CaSCW11 is reduced in both yeast and hyphal forms. The CaAce2 protein is localized to the daughter nucleus of large budded cells at the end of mitosis. C. albicans Ace2p therefore plays a major role in morphogenesis and adherence and resembles S. cerevisiae Ace2p in function",
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Kelly, MT, MacCallum, D, Clancy, S, Odds, FC, Brown, A & Butler, G 2004, 'The Candida Albicans CaACE2 Gene Affects Morphogenesis, Adherence and Virulence', Molecular Microbiology, vol. 53, no. 3, pp. 969-983. https://doi.org/10.1111/j.1365-2958.2004.04185.x

The Candida Albicans CaACE2 Gene Affects Morphogenesis, Adherence and Virulence. / Kelly, Mary T.; MacCallum, Donna; Clancy, Susanne; Odds, Frank C.; Brown, Alistar; Butler, Geraldine.

In: Molecular Microbiology, Vol. 53, No. 3, 2004, p. 969-983.

Research output: Contribution to journalArticle

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AB - Morphogenesis between yeast and hyphal growth is a characteristic associated with virulence in Candida albicans and involves changes in the cell wall. In Saccharomyces cerevisiae, the transcription factor pair Ace2p and Swi5p are key regulators of cell wall metabolism. Here, we have characterized the CaACE2 gene, which encodes the only C. albicans homologue of S. cerevisiae ACE2 and SWI5. Deleting CaACE2 results in a defect in cell separation, increased invasion of solid agar medium and inappropriate pseudohyphal growth, even in the absence of external inducers. The mutant cells have reduced adherence to plastic surfaces and generate biofilms with distinctly different morphology from wild-type cells. They are also avirulent in a mouse model. Deleting CaACE2 has no effect on expression of the chitinase gene CHT2, but expression of CHT3 and the putative cell wall genes CaDSE1 and CaSCW11 is reduced in both yeast and hyphal forms. The CaAce2 protein is localized to the daughter nucleus of large budded cells at the end of mitosis. C. albicans Ace2p therefore plays a major role in morphogenesis and adherence and resembles S. cerevisiae Ace2p in function

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