The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation

E. A. McLaughlin, W. C.L. Ford, M. G.R. Hull

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44 Citations (Scopus)

Abstract

The contribution of the toxicity of glycerol egg yolk citrate (GEYC) cryopreservative medium to the loss of function of human spermatozoa during cryopreservation was determined by investigating the effect of mixing semen with the medium on sperm motility. The percentage of progressively motile spermatozoa, velocity (μm s-1) and lateral head displacement (μm) (mean ± SEM, n = 28) were 55 ± 4.1, 47 ± 2.7, 4.4 ± 0.2, and 32 ± 3.8, 40 ± 2.5, 3.6 ± 0.25 and 15 ± 2.5, 28 ± 1.1, 2.8 ± 0.15 in suspensions of washed spermatozoa prepared from fresh, GEYC-treated and frozen-thawed semen, respectively. The variables changed only slightly after incubation for 3h. The toxicity of GEYC did not vary significantly between samples which survived the complete freeze-thaw cycle well or very poorly. The toxicity of GEYC is responsible for about 50% of the loss of progressively motile spermatozoa during the complete cryopreservation process, but has little effect on the quality of motility. Susceptibility to GEYC does not explain observed differences in the ability of semen samples to survive freezing.

Original languageEnglish
Pages (from-to)749-754
Number of pages6
JournalJournal of Reproduction and Fertility
Volume95
Issue number3
DOIs
Publication statusPublished - 1992
Externally publishedYes

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Egg Yolk
Sperm Motility
Cryopreservation
Citric Acid
Glycerol
Spermatozoa
Semen
Semen Preservation
Freezing
Suspensions

Cite this

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title = "The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation",
abstract = "The contribution of the toxicity of glycerol egg yolk citrate (GEYC) cryopreservative medium to the loss of function of human spermatozoa during cryopreservation was determined by investigating the effect of mixing semen with the medium on sperm motility. The percentage of progressively motile spermatozoa, velocity (μm s-1) and lateral head displacement (μm) (mean ± SEM, n = 28) were 55 ± 4.1, 47 ± 2.7, 4.4 ± 0.2, and 32 ± 3.8, 40 ± 2.5, 3.6 ± 0.25 and 15 ± 2.5, 28 ± 1.1, 2.8 ± 0.15 in suspensions of washed spermatozoa prepared from fresh, GEYC-treated and frozen-thawed semen, respectively. The variables changed only slightly after incubation for 3h. The toxicity of GEYC did not vary significantly between samples which survived the complete freeze-thaw cycle well or very poorly. The toxicity of GEYC is responsible for about 50{\%} of the loss of progressively motile spermatozoa during the complete cryopreservation process, but has little effect on the quality of motility. Susceptibility to GEYC does not explain observed differences in the ability of semen samples to survive freezing.",
keywords = "Cryopreservation, Donor insemination, Glycerol, Human, Motility, Spermatozoa",
author = "McLaughlin, {E. A.} and Ford, {W. C.L.} and Hull, {M. G.R.}",
year = "1992",
doi = "10.1530/jrf.0.0950749",
language = "English",
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T1 - The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation

AU - McLaughlin, E. A.

AU - Ford, W. C.L.

AU - Hull, M. G.R.

PY - 1992

Y1 - 1992

N2 - The contribution of the toxicity of glycerol egg yolk citrate (GEYC) cryopreservative medium to the loss of function of human spermatozoa during cryopreservation was determined by investigating the effect of mixing semen with the medium on sperm motility. The percentage of progressively motile spermatozoa, velocity (μm s-1) and lateral head displacement (μm) (mean ± SEM, n = 28) were 55 ± 4.1, 47 ± 2.7, 4.4 ± 0.2, and 32 ± 3.8, 40 ± 2.5, 3.6 ± 0.25 and 15 ± 2.5, 28 ± 1.1, 2.8 ± 0.15 in suspensions of washed spermatozoa prepared from fresh, GEYC-treated and frozen-thawed semen, respectively. The variables changed only slightly after incubation for 3h. The toxicity of GEYC did not vary significantly between samples which survived the complete freeze-thaw cycle well or very poorly. The toxicity of GEYC is responsible for about 50% of the loss of progressively motile spermatozoa during the complete cryopreservation process, but has little effect on the quality of motility. Susceptibility to GEYC does not explain observed differences in the ability of semen samples to survive freezing.

AB - The contribution of the toxicity of glycerol egg yolk citrate (GEYC) cryopreservative medium to the loss of function of human spermatozoa during cryopreservation was determined by investigating the effect of mixing semen with the medium on sperm motility. The percentage of progressively motile spermatozoa, velocity (μm s-1) and lateral head displacement (μm) (mean ± SEM, n = 28) were 55 ± 4.1, 47 ± 2.7, 4.4 ± 0.2, and 32 ± 3.8, 40 ± 2.5, 3.6 ± 0.25 and 15 ± 2.5, 28 ± 1.1, 2.8 ± 0.15 in suspensions of washed spermatozoa prepared from fresh, GEYC-treated and frozen-thawed semen, respectively. The variables changed only slightly after incubation for 3h. The toxicity of GEYC did not vary significantly between samples which survived the complete freeze-thaw cycle well or very poorly. The toxicity of GEYC is responsible for about 50% of the loss of progressively motile spermatozoa during the complete cryopreservation process, but has little effect on the quality of motility. Susceptibility to GEYC does not explain observed differences in the ability of semen samples to survive freezing.

KW - Cryopreservation

KW - Donor insemination

KW - Glycerol

KW - Human

KW - Motility

KW - Spermatozoa

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