TY - JOUR
T1 - The diagnostic sensitivity of circulating cardio-enriched microRNAs is increased after normalization of high-density lipoprotein levels
AU - Ram, Thrishila Parshu
AU - Fomison-Nurse, Ingrid
AU - Gandhi, Sophie
AU - Coffey, Sean
AU - Saxena, Pankaj
AU - Galvin, Ivor
AU - Bunton, Richard
AU - Williams, Michael J.A.
AU - Lamberts, Regis R.
AU - Katare, Rajesh
N1 - Funding Information:
This study was supported by the research project grants from the Heart Foundation (1600), Otago School of Medical Sciences Dean's Bequest funding and Physiology Development Research Fund, RSNZ catalyst Seed Funding (CSG-U001601), Department of Physiology, University of Otago, New Zealand.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Background Emerging evidence suggests that microRNAs (miRs) could be a potential biomarker to identify early molecular alterations in the heart. HDL are the major carriers for miRs into the circulation. This study tested whether changes in the level of HDL could affect the diagnostic sensitivity of miRs. Methods and results Peripheral blood samples were collected from 20 diabetic and 22 age and gender matched non-diabetic patients undergoing coronary artery bypass graft (CABG) surgery for ischemic heart disease (IHD). Total RNA was extracted from the separated plasma and stored in − 80 °C. Reverse transcription and amplification using specific primers against cardio-enriched miR-1, -34a, -126, -133, and -499 showed significant correlation between HDL levels and miR-1, -133 and -499. Importantly, normalization of miR levels with HDL showed a significant downregulation of miR-1, -133 and -499 in diabetic plasma, which was not observed before normalization with HDL levels. Conclusion Normalization of circulating miR levels with HDL increases the diagnostic sensitivity of circulating miRs.
AB - Background Emerging evidence suggests that microRNAs (miRs) could be a potential biomarker to identify early molecular alterations in the heart. HDL are the major carriers for miRs into the circulation. This study tested whether changes in the level of HDL could affect the diagnostic sensitivity of miRs. Methods and results Peripheral blood samples were collected from 20 diabetic and 22 age and gender matched non-diabetic patients undergoing coronary artery bypass graft (CABG) surgery for ischemic heart disease (IHD). Total RNA was extracted from the separated plasma and stored in − 80 °C. Reverse transcription and amplification using specific primers against cardio-enriched miR-1, -34a, -126, -133, and -499 showed significant correlation between HDL levels and miR-1, -133 and -499. Importantly, normalization of miR levels with HDL showed a significant downregulation of miR-1, -133 and -499 in diabetic plasma, which was not observed before normalization with HDL levels. Conclusion Normalization of circulating miR levels with HDL increases the diagnostic sensitivity of circulating miRs.
KW - Biomarkers
KW - Circulating miRs
KW - Diabetes
KW - HDL
KW - Ischemic heart disease
UR - http://www.scopus.com/inward/record.url?scp=85011397616&partnerID=8YFLogxK
U2 - 10.1016/j.ijcard.2017.01.119
DO - 10.1016/j.ijcard.2017.01.119
M3 - Article
C2 - 28169059
AN - SCOPUS:85011397616
SN - 0167-5273
VL - 236
SP - 498
EP - 500
JO - International Journal of Cardiology
JF - International Journal of Cardiology
ER -
