TY - JOUR
T1 - The effect of casein protein prior to sleep on fat metabolism in obese men
AU - Kinsey, Amber W.
AU - Cappadona, Stacy R.
AU - Panton, Lynn B.
AU - Allman, Brittany R.
AU - Contreras, Robert J.
AU - Hickner, Robert C.
AU - Ormsbee, Michael J.
N1 - Funding Information:
The authors would like to thank Eduarda Machado and Ashley Wahl for their assistance with participant recruitment and data collection. We would also like to thank Marissa Abegg for assistance with analyzing sleep data. Finally, we would like to thank our participants for their time and involvement in our study. This research was supported by funds from the Florida State University College of Human Sciences, the Florida State University Graduate School and Dymatize Nutrition Sport Performance Institute. A.W.K. was funded by the NIH T32DK062710 grant during the journal preparation of this manuscript.
Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2016/8
Y1 - 2016/8
N2 - We have previously shown that ingesting protein at night before sleep is either beneficial or non-detrimental to metabolism, health, and body composition in obese women. However, the overnight protein-induced lipolytic actions and mechanism for improved metabolism and body composition have not been fully established. Therefore, in a crossover design, twelve obese men (age, 27.0 ± 2.2 years) were randomly assigned to ingest (within 30 min of sleep) casein protein (CAS, 120 kcal) or a non-nutritive placebo (PLA) before going to sleep. Markers of fat metabolism (lipolysis, substrate utilization, growth hormone), insulin, glucose, resting energy expenditure (REE), and appetite (questionnaire and ghrelin) were measured. During sleep and the next morning, interstitial glycerol from the subcutaneous abdominal adipose tissue (SCAAT) was measured using microdialysis. There were no differences in SCAAT glycerol (overnight: CAS, 177.4 ± 26.7; PLA, 183.8 ± 20.2 μmol/L; morning: CAS, 171.6 ± 19.1; PLA, 161.5 ± 18.6 μmol/L), substrate utilization, REE, or any blood markers between CAS and PLA. Desire to eat was greater for CAS compared to baseline (p = 0.03), but not different from PLA (baseline: 39 ± 6, CAS: 62 ± 8, PLA: 55 ± 5 mm). CAS consumption before sleep did not affect fat or glucose metabolism, REE, or suppress appetite in hyperinsulemic obese men. CAS may be consumed before sleep without impeding overnight or morning fat metabolism in young, obese men.
AB - We have previously shown that ingesting protein at night before sleep is either beneficial or non-detrimental to metabolism, health, and body composition in obese women. However, the overnight protein-induced lipolytic actions and mechanism for improved metabolism and body composition have not been fully established. Therefore, in a crossover design, twelve obese men (age, 27.0 ± 2.2 years) were randomly assigned to ingest (within 30 min of sleep) casein protein (CAS, 120 kcal) or a non-nutritive placebo (PLA) before going to sleep. Markers of fat metabolism (lipolysis, substrate utilization, growth hormone), insulin, glucose, resting energy expenditure (REE), and appetite (questionnaire and ghrelin) were measured. During sleep and the next morning, interstitial glycerol from the subcutaneous abdominal adipose tissue (SCAAT) was measured using microdialysis. There were no differences in SCAAT glycerol (overnight: CAS, 177.4 ± 26.7; PLA, 183.8 ± 20.2 μmol/L; morning: CAS, 171.6 ± 19.1; PLA, 161.5 ± 18.6 μmol/L), substrate utilization, REE, or any blood markers between CAS and PLA. Desire to eat was greater for CAS compared to baseline (p = 0.03), but not different from PLA (baseline: 39 ± 6, CAS: 62 ± 8, PLA: 55 ± 5 mm). CAS consumption before sleep did not affect fat or glucose metabolism, REE, or suppress appetite in hyperinsulemic obese men. CAS may be consumed before sleep without impeding overnight or morning fat metabolism in young, obese men.
KW - Appetite
KW - Casein
KW - Microdialysis
KW - Nighttime eating
KW - Obesity
KW - Sleep
UR - http://www.scopus.com/inward/record.url?scp=84980347534&partnerID=8YFLogxK
U2 - 10.3390/nu8080452
DO - 10.3390/nu8080452
M3 - Article
C2 - 27472361
AN - SCOPUS:84980347534
SN - 2072-6643
VL - 8
SP - 1
EP - 15
JO - Nutrients
JF - Nutrients
IS - 8
M1 - 452
ER -
